环介导等温扩增技术在致病微生物检测中的应用
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摘要
环介导等温扩增(LAMP)是一种新兴的核酸扩增方法,由于其具有高灵敏、高特异、操作简单、检测快速、价格低廉等诸多优势,现已成为分子生物学诊断技术的重要组成部分,并广泛应用于基层实验室及野外致病微生物的在快速检测致病微生物中的应用及新进展,希望能为其发展提供参考。
Loop-mediated isothermal amplification(LAMP) is the most popular and widely used isothermalbased detection method because of rapid and easy performation, and greater sensitivity and lower cost. At present,LAMP is widely employed in both laboratory and field conditions. In this review, we will give an overview of the cutting-edge progress of LAMP in the rapid detection of pathogenic microorganisms, providing a reference for its development in next few years.
Loop-mediated isothermal amplification(LAMP) is the most popular and widely used isothermalbased detection method because of rapid and easy performation, and greater sensitivity and lower cost. At present,LAMP is widely employed in both laboratory and field conditions. In this review, we will give an overview of the cutting-edge progress of LAMP in the rapid detection of pathogenic microorganisms, providing a reference for its development in next few years.
引文
[1] Notomi T,Okayama H,Masubuchi H, et al. Loop-mediated isothermal amplification of DNA[J]. Nucleic Acids Res, 2000,28(12):E63.
[2] Notomi T,Mori Y,Tomita N, et al. Loop-mediated isothermal amplification(LAMP):principle, features,and future prospects[J]. J Microbiol, 2015,53(1):1-5.
[3] Nagamine K,Hase T,Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers[J]. Mol Cell Probes, 2002,16(3):223-229.
[4] Sahoo P R, Sethy K, Mohapatra S, et al. Loop mediated isothermal amplification:an innovative gene amplification technique for animal diseases[J]. Vet World,2016,9(5):465-469.
[5] Karthik K, Rathore R, Thomas P, et al. New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination[J]. MethodsX, 2014,1:137-143.
[6] Tanner N A, Zhang Y,Evans T C,Jr. Visual detection of isothermal nucleic acid amplification using pHsensitive dyes[J]. Biotechniques, 2015,58(2):59-68.
[7] Lin Z, Zhang Y, Zhang H, et al. Comparison of loopmediated isothermal amplification(LAMP)and realtime PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis[J]. Vet Parasitol, 2012,185(2-4):296-300.
[8] Francois P,Tangomo M, Hibbs J,et al. Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications[J]. FEMS Immunol Med Microbiol, 2011,62(1):41-48.
[9] Pham N T, Trinh Q D, Khamrin P, et al. Loop-mediated isothermal amplification(LAMP)for detection of Campylobacter jejuni and C.coli in Thai children with diarrhea[J]. Jpn J Infect Dis, 2015,68(5):432-433.
[10] Fan F, Yan M, Du P, et al. Rapid and sensitive Salmonella typhi detection in blood and fecal samples using reverse transcription loop-mediated isothermal amplification[J]. Foodborne Pathog Dis, 2015,12(9):778-786.
[11] Lee D,Kim E J, Kilgore P E,et al. A novel loopmediated isothermal amplification assay for serogroup identification of Neisseria meningitidis in cerebrospinal fluid[J]. Front Microbiol, 2015,6:1548.
[12] Birmpa A, Kalogeropoulos K, Kokkinos P, et al. Evaluation of two loop-mediated isothermal amplification methods for the detection of Salmonella enteritidis and Listeria monocytogenes in artificially contaminated ready-to-eat fresh produce[J]. Ital J Food Saf,2015,4(3):5383.
[13] Bakhtiari S,Alvandi A, Pajavand H, et al. Development and diagnostic evaluation of loop-mediated isothermal amplification using a new gene target for rapid detection of Helicobacter pylori[J]. Jundishapur J Microbiol, 2016,9(5):e28831.
[14] Gong J,Zhuang L, Zhang D, et al. Establishment ofa multiplex loop-mediated isothermal amplification method for rapid detection of sulfonamide resistance genes(sull,sul2, sul3)in clinical enterobacteriaceae isolates from poultry[J]. Foodborne Pathog Dis, 2018,15(7):413-419.
[15] Lee M F, Chen Y J, Hsu H J, et al. One-tube loopmediated isothermal amplification combined with restriction endonuclease digestion and ELISA for colorimetric detection of resistance to isoniazid, ethambutol and streptomycin in Mycobacterium tuberculosis isolates[J]. J Microbiol Methods, 2010,83(1):53-58.
[16] Liu N, Zou D, Dong D, et al. Development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus[J]. Sci Rep, 2017,7:45601.
[17] Duarte C, Salm E, Dorvel B, et al. On-chip parallel detection of foodborne pathogens using loop-mediated isothermal amplification[J]. Biomed Microdevices, 2013,15(5):821-30.
[18] Yan G,Jun L,Kangchen Z, et al. Rapid and visual detection of human enterovirus coxsackievirus A16 by reverse transcription loop-mediated isothermal amplification combined with lateral flow device[J]. Lett Appl Microbiol, 2015,61(6):531-537.
[19] Zhao N, Liu J, Sun D. Detection of HCV genotypes1b and 2a by a reverse transcription loop-mediated isothermal amplification assay[J]. J Med Virol, 2017,89(6):1048-1054.
[20] Hu S F, Li M, Zhong L L, et al. Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1-4[J]. BMC Microbiol, 2015,15:265.
[21] Oloniniyi 0 K, Kurosaki Y,Miyamoto H, et al. Rapid detection of all known ebolavirus species by reverse transcription-loop-mediated isothermal amplification(RT-LAMP)[J]. J Virol Methods, 2017,246:8-14.
[22] Chotiwan N, Brewster C D, Magalhaes T, et al. Rapid and specific detection of Asian-and African-lineage Zika viruses[J]. Sci Translational Med, 2017,9(388):eaag05382017.
[23] Lee S H, Baek Y H, Kim Y H, et al. One-pot reverse transcriptional loop-mediated isothermal amplification(RT-LAMP)for detecting MERS-CoV[J]. Front Microbiol, 2016,7:2166.
[24] Kurosaki Y, Magassouba N, Oloniniyi 0 K, et al. Development and evaluation of reverse transcription loopmediated isothermal amplification(RT-LAMP)assay coupled with a portable device for rapid diagnosis of Ebola virus disease in Guinea[J]. PLoS Negl Trop Dis, 2016,10(2):e0004472.
[25] Ocwieja K E, Sherrill-Mix S,Liu C,et al. A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes[J].PLoS One, 2015,10(2):e0117852.
[26] Damhorst G L. Duarte-Guevara C. Chen W, et al.Smartphone-imaged HIV-1 reverse-transcription loopmediated isothermal amplification(RT-LAMP)on a chip from whole blood[J]. Engineering(Beijing), 2015,1(3):324-335.
[27] Liu H,Li X T,Hu B,et al. Development of reverse transcription loop-mediated isothermal amplification for rapid detection of Batai virus in cattle and mosquitoes[J]. Vector Borne Zoonotic Dis, 2016,16(6):415-422.
[28] Peng D, Xie J, Qiang W, et al. One-step reverse transcription loop-mediated isothermal amplification assay for detection of apple chlorotic leaf spot virus[J]. J Virol Methods, 2017,248:154-158.
[29] Lau T L, Lai M Y, Teoh B T, et al. Colorimetric detection of Dengue by single tube reverse-transcription loop-mediated isothermal amplification[J]. PLoS One,2015,10(9):e0138694.
[30] Nzelu C O,Caceres A G,Guerrero-Quincho S,et al.A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification(LAMP)combined with an FTA card as a direct sampling tool[J]. Acta Trop, 2016,153:116-119.
[31] Poole C B, Ettwiller L,Tanner N A, et al. Genome filtering for new DNA biomarkers of Loa loa infection suitable for loop-mediated isothermal amplification[J].PLoS One, 2015,10(9):e0139286.
[32] Nkouawa A, Sako Y,Okamoto M, et al. Simple identification of human taenia species by multiplex loop-mediated isothermal amplification in combination with dot enzyme-linked immunosorbent assay[J]. Am J Trop Med Hyg, 2016,94(6):1318-1323.
[33] Mwendwa F, Mbae C K, Kinyua J, et al. Stem loopmediated isothermal amplification test:comparative analysis with classical LAMP and PCR in detectionof Entamoeba histolytica in Kenya[J]. BMC Res Notes, 2017,10(1):142.
[34] Nakayama T,Yamazaki T, Yo A, et al. Detection of fungi from an indoor environment using loop-mediated isothermal amplification(LAMP)method[J]. Biocontrol Sci, 2017,22(2):97-104.
[35] Karakkat B B, Hockemeyer K, Franchett M, et al. Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification[J]. J Microbiol Methods, 2018,151:90-98.
[36] Luo J, Taniwaki M H,Iamanaka B T,et al. Application of loop-mediated isothermal amplification assays for direct identification of pure cultures of Aspergillus flavus, A.nomius, and A.caelatus and for their rapid detection in shelled Brazil nuts[J]. Int J Food Microbiol, 2014,172:5-12.
[37] Salinas N R, Little D P. Electric LAMP:virtual loopmediated isothermal amplification[J]. ISRN Bioinform,2012,2012:696758.
[38] Chander Y, Koelbl J,Puckett J,et al. A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification(LAMP)[J]. Front Microbiol,2014,5:395.
[39] Dukes J P, King D P, Alexandersen S. Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus[J].Arch Virol, 2006,151(6):1093-1106.
[40] Huang H L, Zhu P, Zhou C X, et al. The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Karlodinium veneficum[J]. Harmful Algae, 2017,62:20-29.
[41] Yin H Y, Fang T J, Wen H W. Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea and seb genes of enterotoxic Staphylococcus aureus[J]. Lett Appl Microbiol,2016,63(1):16-24.
[42] Xu C, Feng Y,Chen Y, et al. Rapid detection of measles virus using reverse transcription loop-mediated isothermal amplification coupled with a disposable lateral flow device[J]. Diagn Microbiol Infect Dis, 2016,85(2):168-173.
[43] Yang Y,Li Q,Wang S, et al. Rapid and sensitive de-tection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick[J]. Vet Parasitol, 2016,219:71-76.
[44] Kongkasuriyachai D, Yongkiettrakul S, Kiatpathomchai W, et al. Loop-mediated isothermal amplification and LFD combination for detection of Plasmodium falciparum and Plasmodium vivax[J]. Methods Mol Biol,2017,1572:431-443.
[45] Hayashida K, Kajino K, Hachaambwa L, et al. Direct blood dry LAMP:a rapid, stable, and easy diagnostic tool for human African trypanosomiasis[J]. PLoS Negl Trop Dis, 2015,9(3):e0003578.
[46] Chen H W, Weissenberger G, Ching W M. Development of lyophilized loop-mediated isothermal amplification reagents for the detection of leptospira[J]. Mil Med, 2016,181(5 Suppl):227-231.
[47] Carter C, Akrami K, Hall D,et al. Lyophilized visually readable loop-mediated isothermal reverse transcriptase nucleic acid amplification test for detection Ebola Zaire RNA[J]. J Virol Methods, 2017,244:32-38.
[48] Dhama K, Karthik K, Chakraborty S, et al. Loop-mediated isothermal amplification of DNA(LAMP):a new diagnostic tool lights the world of diagnosis of animal and human pathogens:a review[J]. Pak J Biol Sci,2014,17(2):151-166.
[49] Watts M R, James G, Sultana Y, et al. A loop-mediated isothermal amplification(LAMP)assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye[J]. Am J Trop Med Hyg,2014,90(2):306-311.
[50] Fischbach J, Xander N C, Frohme M, et al. Shining a light on LAMP assays-a comparison of LAMP visualization methods including the novel use of berberine[J]. Biotechniques, 2015,58(4):189-194.
[51] Bista B R, Isbwad C, Wadowsky R M, et al. Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus[J]. J Clin Microbiol, 2007,45(5):1581-1587.
[52] Wastling S L, Picozzi K, Kakembo A S, et al. LAMP for human African trypanosomiasis:a comparative study of detection formats[J]. PLoS Negl Trop Dis,2010,4(11):e865.
[2] Notomi T,Mori Y,Tomita N, et al. Loop-mediated isothermal amplification(LAMP):principle, features,and future prospects[J]. J Microbiol, 2015,53(1):1-5.
[3] Nagamine K,Hase T,Notomi T. Accelerated reaction by loop-mediated isothermal amplification using loop primers[J]. Mol Cell Probes, 2002,16(3):223-229.
[4] Sahoo P R, Sethy K, Mohapatra S, et al. Loop mediated isothermal amplification:an innovative gene amplification technique for animal diseases[J]. Vet World,2016,9(5):465-469.
[5] Karthik K, Rathore R, Thomas P, et al. New closed tube loop mediated isothermal amplification assay for prevention of product cross-contamination[J]. MethodsX, 2014,1:137-143.
[6] Tanner N A, Zhang Y,Evans T C,Jr. Visual detection of isothermal nucleic acid amplification using pHsensitive dyes[J]. Biotechniques, 2015,58(2):59-68.
[7] Lin Z, Zhang Y, Zhang H, et al. Comparison of loopmediated isothermal amplification(LAMP)and realtime PCR method targeting a 529-bp repeat element for diagnosis of toxoplasmosis[J]. Vet Parasitol, 2012,185(2-4):296-300.
[8] Francois P,Tangomo M, Hibbs J,et al. Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications[J]. FEMS Immunol Med Microbiol, 2011,62(1):41-48.
[9] Pham N T, Trinh Q D, Khamrin P, et al. Loop-mediated isothermal amplification(LAMP)for detection of Campylobacter jejuni and C.coli in Thai children with diarrhea[J]. Jpn J Infect Dis, 2015,68(5):432-433.
[10] Fan F, Yan M, Du P, et al. Rapid and sensitive Salmonella typhi detection in blood and fecal samples using reverse transcription loop-mediated isothermal amplification[J]. Foodborne Pathog Dis, 2015,12(9):778-786.
[11] Lee D,Kim E J, Kilgore P E,et al. A novel loopmediated isothermal amplification assay for serogroup identification of Neisseria meningitidis in cerebrospinal fluid[J]. Front Microbiol, 2015,6:1548.
[12] Birmpa A, Kalogeropoulos K, Kokkinos P, et al. Evaluation of two loop-mediated isothermal amplification methods for the detection of Salmonella enteritidis and Listeria monocytogenes in artificially contaminated ready-to-eat fresh produce[J]. Ital J Food Saf,2015,4(3):5383.
[13] Bakhtiari S,Alvandi A, Pajavand H, et al. Development and diagnostic evaluation of loop-mediated isothermal amplification using a new gene target for rapid detection of Helicobacter pylori[J]. Jundishapur J Microbiol, 2016,9(5):e28831.
[14] Gong J,Zhuang L, Zhang D, et al. Establishment ofa multiplex loop-mediated isothermal amplification method for rapid detection of sulfonamide resistance genes(sull,sul2, sul3)in clinical enterobacteriaceae isolates from poultry[J]. Foodborne Pathog Dis, 2018,15(7):413-419.
[15] Lee M F, Chen Y J, Hsu H J, et al. One-tube loopmediated isothermal amplification combined with restriction endonuclease digestion and ELISA for colorimetric detection of resistance to isoniazid, ethambutol and streptomycin in Mycobacterium tuberculosis isolates[J]. J Microbiol Methods, 2010,83(1):53-58.
[16] Liu N, Zou D, Dong D, et al. Development of a multiplex loop-mediated isothermal amplification method for the simultaneous detection of Salmonella spp. and Vibrio parahaemolyticus[J]. Sci Rep, 2017,7:45601.
[17] Duarte C, Salm E, Dorvel B, et al. On-chip parallel detection of foodborne pathogens using loop-mediated isothermal amplification[J]. Biomed Microdevices, 2013,15(5):821-30.
[18] Yan G,Jun L,Kangchen Z, et al. Rapid and visual detection of human enterovirus coxsackievirus A16 by reverse transcription loop-mediated isothermal amplification combined with lateral flow device[J]. Lett Appl Microbiol, 2015,61(6):531-537.
[19] Zhao N, Liu J, Sun D. Detection of HCV genotypes1b and 2a by a reverse transcription loop-mediated isothermal amplification assay[J]. J Med Virol, 2017,89(6):1048-1054.
[20] Hu S F, Li M, Zhong L L, et al. Development of reverse-transcription loop-mediated isothermal amplification assay for rapid detection and differentiation of dengue virus serotypes 1-4[J]. BMC Microbiol, 2015,15:265.
[21] Oloniniyi 0 K, Kurosaki Y,Miyamoto H, et al. Rapid detection of all known ebolavirus species by reverse transcription-loop-mediated isothermal amplification(RT-LAMP)[J]. J Virol Methods, 2017,246:8-14.
[22] Chotiwan N, Brewster C D, Magalhaes T, et al. Rapid and specific detection of Asian-and African-lineage Zika viruses[J]. Sci Translational Med, 2017,9(388):eaag05382017.
[23] Lee S H, Baek Y H, Kim Y H, et al. One-pot reverse transcriptional loop-mediated isothermal amplification(RT-LAMP)for detecting MERS-CoV[J]. Front Microbiol, 2016,7:2166.
[24] Kurosaki Y, Magassouba N, Oloniniyi 0 K, et al. Development and evaluation of reverse transcription loopmediated isothermal amplification(RT-LAMP)assay coupled with a portable device for rapid diagnosis of Ebola virus disease in Guinea[J]. PLoS Negl Trop Dis, 2016,10(2):e0004472.
[25] Ocwieja K E, Sherrill-Mix S,Liu C,et al. A reverse transcription loop-mediated isothermal amplification assay optimized to detect multiple HIV subtypes[J].PLoS One, 2015,10(2):e0117852.
[26] Damhorst G L. Duarte-Guevara C. Chen W, et al.Smartphone-imaged HIV-1 reverse-transcription loopmediated isothermal amplification(RT-LAMP)on a chip from whole blood[J]. Engineering(Beijing), 2015,1(3):324-335.
[27] Liu H,Li X T,Hu B,et al. Development of reverse transcription loop-mediated isothermal amplification for rapid detection of Batai virus in cattle and mosquitoes[J]. Vector Borne Zoonotic Dis, 2016,16(6):415-422.
[28] Peng D, Xie J, Qiang W, et al. One-step reverse transcription loop-mediated isothermal amplification assay for detection of apple chlorotic leaf spot virus[J]. J Virol Methods, 2017,248:154-158.
[29] Lau T L, Lai M Y, Teoh B T, et al. Colorimetric detection of Dengue by single tube reverse-transcription loop-mediated isothermal amplification[J]. PLoS One,2015,10(9):e0138694.
[30] Nzelu C O,Caceres A G,Guerrero-Quincho S,et al.A rapid molecular diagnosis of cutaneous leishmaniasis by colorimetric malachite green-loop-mediated isothermal amplification(LAMP)combined with an FTA card as a direct sampling tool[J]. Acta Trop, 2016,153:116-119.
[31] Poole C B, Ettwiller L,Tanner N A, et al. Genome filtering for new DNA biomarkers of Loa loa infection suitable for loop-mediated isothermal amplification[J].PLoS One, 2015,10(9):e0139286.
[32] Nkouawa A, Sako Y,Okamoto M, et al. Simple identification of human taenia species by multiplex loop-mediated isothermal amplification in combination with dot enzyme-linked immunosorbent assay[J]. Am J Trop Med Hyg, 2016,94(6):1318-1323.
[33] Mwendwa F, Mbae C K, Kinyua J, et al. Stem loopmediated isothermal amplification test:comparative analysis with classical LAMP and PCR in detectionof Entamoeba histolytica in Kenya[J]. BMC Res Notes, 2017,10(1):142.
[34] Nakayama T,Yamazaki T, Yo A, et al. Detection of fungi from an indoor environment using loop-mediated isothermal amplification(LAMP)method[J]. Biocontrol Sci, 2017,22(2):97-104.
[35] Karakkat B B, Hockemeyer K, Franchett M, et al. Detection of root-infecting fungi on cool-season turfgrasses using loop-mediated isothermal amplification and recombinase polymerase amplification[J]. J Microbiol Methods, 2018,151:90-98.
[36] Luo J, Taniwaki M H,Iamanaka B T,et al. Application of loop-mediated isothermal amplification assays for direct identification of pure cultures of Aspergillus flavus, A.nomius, and A.caelatus and for their rapid detection in shelled Brazil nuts[J]. Int J Food Microbiol, 2014,172:5-12.
[37] Salinas N R, Little D P. Electric LAMP:virtual loopmediated isothermal amplification[J]. ISRN Bioinform,2012,2012:696758.
[38] Chander Y, Koelbl J,Puckett J,et al. A novel thermostable polymerase for RNA and DNA loop-mediated isothermal amplification(LAMP)[J]. Front Microbiol,2014,5:395.
[39] Dukes J P, King D P, Alexandersen S. Novel reverse transcription loop-mediated isothermal amplification for rapid detection of foot-and-mouth disease virus[J].Arch Virol, 2006,151(6):1093-1106.
[40] Huang H L, Zhu P, Zhou C X, et al. The development of loop-mediated isothermal amplification combined with lateral flow dipstick for detection of Karlodinium veneficum[J]. Harmful Algae, 2017,62:20-29.
[41] Yin H Y, Fang T J, Wen H W. Combined multiplex loop-mediated isothermal amplification with lateral flow assay to detect sea and seb genes of enterotoxic Staphylococcus aureus[J]. Lett Appl Microbiol,2016,63(1):16-24.
[42] Xu C, Feng Y,Chen Y, et al. Rapid detection of measles virus using reverse transcription loop-mediated isothermal amplification coupled with a disposable lateral flow device[J]. Diagn Microbiol Infect Dis, 2016,85(2):168-173.
[43] Yang Y,Li Q,Wang S, et al. Rapid and sensitive de-tection of Babesia bovis and Babesia bigemina by loop-mediated isothermal amplification combined with a lateral flow dipstick[J]. Vet Parasitol, 2016,219:71-76.
[44] Kongkasuriyachai D, Yongkiettrakul S, Kiatpathomchai W, et al. Loop-mediated isothermal amplification and LFD combination for detection of Plasmodium falciparum and Plasmodium vivax[J]. Methods Mol Biol,2017,1572:431-443.
[45] Hayashida K, Kajino K, Hachaambwa L, et al. Direct blood dry LAMP:a rapid, stable, and easy diagnostic tool for human African trypanosomiasis[J]. PLoS Negl Trop Dis, 2015,9(3):e0003578.
[46] Chen H W, Weissenberger G, Ching W M. Development of lyophilized loop-mediated isothermal amplification reagents for the detection of leptospira[J]. Mil Med, 2016,181(5 Suppl):227-231.
[47] Carter C, Akrami K, Hall D,et al. Lyophilized visually readable loop-mediated isothermal reverse transcriptase nucleic acid amplification test for detection Ebola Zaire RNA[J]. J Virol Methods, 2017,244:32-38.
[48] Dhama K, Karthik K, Chakraborty S, et al. Loop-mediated isothermal amplification of DNA(LAMP):a new diagnostic tool lights the world of diagnosis of animal and human pathogens:a review[J]. Pak J Biol Sci,2014,17(2):151-166.
[49] Watts M R, James G, Sultana Y, et al. A loop-mediated isothermal amplification(LAMP)assay for Strongyloides stercoralis in stool that uses a visual detection method with SYTO-82 fluorescent dye[J]. Am J Trop Med Hyg,2014,90(2):306-311.
[50] Fischbach J, Xander N C, Frohme M, et al. Shining a light on LAMP assays-a comparison of LAMP visualization methods including the novel use of berberine[J]. Biotechniques, 2015,58(4):189-194.
[51] Bista B R, Isbwad C, Wadowsky R M, et al. Development of a loop-mediated isothermal amplification assay for rapid detection of BK virus[J]. J Clin Microbiol, 2007,45(5):1581-1587.
[52] Wastling S L, Picozzi K, Kakembo A S, et al. LAMP for human African trypanosomiasis:a comparative study of detection formats[J]. PLoS Negl Trop Dis,2010,4(11):e865.