不同培养基对水稻花药培养力的影响
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摘要
以4份不同籼粳成分的水稻为试验材料,分别以不同基本培养基(GM_8、N_6)、激素(单一激素、复合激素)为变量,设计4种诱导培养基进行花药培养,探讨水稻适宜的花药培养条件。结果表明,GM_8培养基对籼稻材料、偏籼型材料具有较好的花药培养效果,N_6培养基对粳稻具有较好的花药培养效果;添加复合激素培养基处理其愈伤组织数量和发育情况明显优于单一激素处理,说明复合激素处理出愈率高,花药培养效果好。
Four rice cultivars with different indica and japonica components were used as experimental materials,and four induction media were designed with different basic medias(GM_8,N_6)and hormones(single hormone,complex hormones)as variables to study the suitable conditions for anther culture of rice.The results showed that GM_8 medium had better effect for anther culture of indica rice and indica-cline type,and N_6 medium had better effect for anther culture of Japonica rice.The number and developmental condition of callus from medium treated with complex hormones were better than that treated with single hormone,which indicated that complex hormones treatment had higher callus rate and better anther culture effect.
Four rice cultivars with different indica and japonica components were used as experimental materials,and four induction media were designed with different basic medias(GM_8,N_6)and hormones(single hormone,complex hormones)as variables to study the suitable conditions for anther culture of rice.The results showed that GM_8 medium had better effect for anther culture of indica rice and indica-cline type,and N_6 medium had better effect for anther culture of Japonica rice.The number and developmental condition of callus from medium treated with complex hormones were better than that treated with single hormone,which indicated that complex hormones treatment had higher callus rate and better anther culture effect.
引文
[1]王伍梅,台德卫,张效忠,等.水稻高效花药培养技术体系的构建[J].中国农学通报,2009,25(16):65-68.
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[3]陈红,秦瑞珍.提高水稻同源四倍体花药培养愈伤诱导率的研究[J].作物学报,2007,33(1):120-125.
[4]杨跃生,简玉瑜.影响水稻愈伤组织再生植株数量和质量的因素[J].农业生物技术学报,1996,4(2):124-128.
[5]施利利,张欣,郭玉华.影响籼粳稻杂交F1花药培养效果的因素分析[J].华北农学报,2007,22(4):158-161.
[6]郭书巧,唐海娟,王州飞,等.籼粳杂交F1高效花药培养技术体系的建立[J].南京农业大学学报,2006,29(2):1-5.
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[8]韦鹏霄,岑秀芬.水稻花药培养及育种应用研[J].广西农业生物科学,1999,18(1):79-94.
[9]赵成章.水稻试管苗正常化调控技术的研究[C]//胡古,王恒立.植物细胞工程与育种.北京:北京工业大学出版社,1990:352-356.
[10]张林,傅雪琳,陈雄辉.水稻花药培养特性的品种间变异观察[J].福建农业学报,2003,18(2):69-73.
[2]冯双华,赵森,郭家源.不同培养基和激素对超级杂交稻花药培养力的影响[J].西南农业大学学报,2006,28(4):523-525.
[3]陈红,秦瑞珍.提高水稻同源四倍体花药培养愈伤诱导率的研究[J].作物学报,2007,33(1):120-125.
[4]杨跃生,简玉瑜.影响水稻愈伤组织再生植株数量和质量的因素[J].农业生物技术学报,1996,4(2):124-128.
[5]施利利,张欣,郭玉华.影响籼粳稻杂交F1花药培养效果的因素分析[J].华北农学报,2007,22(4):158-161.
[6]郭书巧,唐海娟,王州飞,等.籼粳杂交F1高效花药培养技术体系的建立[J].南京农业大学学报,2006,29(2):1-5.
[7]沈锦骅,李梅芳,陈银全.花药培养在水稻品种改良上的应用[J].中国农业科学,1982,(2):15-19.
[8]韦鹏霄,岑秀芬.水稻花药培养及育种应用研[J].广西农业生物科学,1999,18(1):79-94.
[9]赵成章.水稻试管苗正常化调控技术的研究[C]//胡古,王恒立.植物细胞工程与育种.北京:北京工业大学出版社,1990:352-356.
[10]张林,傅雪琳,陈雄辉.水稻花药培养特性的品种间变异观察[J].福建农业学报,2003,18(2):69-73.