Study on the physiological indices of Pinus sibirica and Pinus koraiensis seedlings under cold stress
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摘要
Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the northern hemisphere,the former has a stronger cold resistance.Research has been limited to the comparison of physiological responses of the two species to cold stress.In this study,5-year-old seedlings of P.sibirica and P.koraiensis were subjected to six temperature treatments,[20 ℃(control),0 ℃,-20 ℃,-40 ℃,-60 ℃,and-80 ℃],under different stress periods(6,12,24,and 48 h).The results showed that differences in each physiological index were significant between P.sibirica and P.koraiensis,except for the permeability of cell membranes,reactive oxygen species,proline and soluble proteins.An ANOVA test indicated that there were extreme differences among the temperatures for each index,stress time and temperature 9 time for most indices.All indices showed a similar trend for P.sibirica and P.koraiensis with decreasing temperature or the extension of stress time.Soluble sugars and proline increased at 0 to-20℃ and then remained unchanged with temperature decline.Other indices showed an increase from 20 to-20℃,stable from-20 to-40℃ and a decrease from-40 to-80℃.All the indices increased and then declined along with the prolonged cold stress time,except for the control.From 0 to-40℃,the permeability of cell membranes,relative conductivity,reactive oxygen species and malonaldehyde of P.koraiensis seedlings were higher than in P.sibirica,but superoxide dismutase,peroxidase,catalase activity and soluble sugars,proline,and soluble proteins content emerged as opposite.This study compared the physiological mechanism responses to cold stress between P.sibirica and P.koraiensis to provide the basis for the introduction,distribution,and genetic improvement of these coniferous species.
Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the northern hemisphere,the former has a stronger cold resistance.Research has been limited to the comparison of physiological responses of the two species to cold stress.In this study,5-year-old seedlings of P.sibirica and P.koraiensis were subjected to six temperature treatments,[20 ℃(control),0 ℃,-20 ℃,-40 ℃,-60 ℃,and-80 ℃],under different stress periods(6,12,24,and 48 h).The results showed that differences in each physiological index were significant between P.sibirica and P.koraiensis,except for the permeability of cell membranes,reactive oxygen species,proline and soluble proteins.An ANOVA test indicated that there were extreme differences among the temperatures for each index,stress time and temperature 9 time for most indices.All indices showed a similar trend for P.sibirica and P.koraiensis with decreasing temperature or the extension of stress time.Soluble sugars and proline increased at 0 to-20℃ and then remained unchanged with temperature decline.Other indices showed an increase from 20 to-20℃,stable from-20 to-40℃ and a decrease from-40 to-80℃.All the indices increased and then declined along with the prolonged cold stress time,except for the control.From 0 to-40℃,the permeability of cell membranes,relative conductivity,reactive oxygen species and malonaldehyde of P.koraiensis seedlings were higher than in P.sibirica,but superoxide dismutase,peroxidase,catalase activity and soluble sugars,proline,and soluble proteins content emerged as opposite.This study compared the physiological mechanism responses to cold stress between P.sibirica and P.koraiensis to provide the basis for the introduction,distribution,and genetic improvement of these coniferous species.
Although Pinus sibirica and Pinus koraiensis are resistant to cold or low temperatures in the cold temperate regions of the northern hemisphere,the former has a stronger cold resistance.Research has been limited to the comparison of physiological responses of the two species to cold stress.In this study,5-year-old seedlings of P.sibirica and P.koraiensis were subjected to six temperature treatments,[20 ℃(control),0 ℃,-20 ℃,-40 ℃,-60 ℃,and-80 ℃],under different stress periods(6,12,24,and 48 h).The results showed that differences in each physiological index were significant between P.sibirica and P.koraiensis,except for the permeability of cell membranes,reactive oxygen species,proline and soluble proteins.An ANOVA test indicated that there were extreme differences among the temperatures for each index,stress time and temperature 9 time for most indices.All indices showed a similar trend for P.sibirica and P.koraiensis with decreasing temperature or the extension of stress time.Soluble sugars and proline increased at 0 to-20℃ and then remained unchanged with temperature decline.Other indices showed an increase from 20 to-20℃,stable from-20 to-40℃ and a decrease from-40 to-80℃.All the indices increased and then declined along with the prolonged cold stress time,except for the control.From 0 to-40℃,the permeability of cell membranes,relative conductivity,reactive oxygen species and malonaldehyde of P.koraiensis seedlings were higher than in P.sibirica,but superoxide dismutase,peroxidase,catalase activity and soluble sugars,proline,and soluble proteins content emerged as opposite.This study compared the physiological mechanism responses to cold stress between P.sibirica and P.koraiensis to provide the basis for the introduction,distribution,and genetic improvement of these coniferous species.
引文
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Bargali K,Bargali SS(2016)Germination capacity of seeds of leguminous plants under water deficit conditions:implication for restoration of degraded lands in kumaun himalaya.Trop Ecol57(3):445-453
Bertrand A,Robitaille G,Nadeau P,Castonguay Y(1999)Influence of ozone on cold acclimation in sugar maple seedlings.Tree Physiol 19(8):527-534
Bhattacharjee S(1996)Short-term heat and cold shock induced proline accumulation in relation to Ca2?involvement in Lycopersicum esculantum(Mill)cultured cells and seedlings.Indian J Plant Physiol 1(1):32-35
Bian SM,Jiang YW(2009)Reactive oxygen species,antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery.Sci Hortic 120(2):264-270
Bowler C,And MVM,Inze D(2003)Superoxide dismutase and stress tolerance.Annu Rev Plant Biol 43(1):83-116
Bradford MM(1976)A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem72(s1-2):248-254
Burke MJ,Gusta LV,Quamme HA,Weiser CJ,Li PH(2003)Freezing and injury in plants.Ann Rev Plant Physiol27(1):507-528
Dalen LS,Johnsen?,Lonneborg A,Yaish MW(2015)Freezing tolerance in Norway spruce,the potential role of pathogenesisrelated proteins.Acta Physiol Plant 37(1):1717
Farhangi-Abriz S,Torabian S(2017)Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress.Ecotoxicol Environ Saf 137:64-70
Foyer CH,Noctor G(2005)Redox homeostasis and antioxidant signaling:a metabolic interface between stress perception and physiological responses.Plant Cell 17(7):1866-1875
Foyer CH,Descourvieres P,Kunert KJ(1994)Protection against oxygen radicals:an important defence mechanism studied in transgenic plants.Plant Cell Environ 17(5):507-523
Garratt LC,Janagoudar BS,Lowe KC,Anthony P,Power JB,Davey MR(2002)Salinity tolerance and antioxidant status in cotton cultures.Free Radic Biol Med 33(4):502-511
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Gill SS,Tuteja N(2010)Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.Plant Physiol Biochem 48(12):909-930
He E,Bai RH,Shen J,Zhao FH,Cao Y(2000)Study on afforestation in the experiment of introducing Pinus Sibirica.J Inn Mong Agric Univ(Nat Sci Ed)21(2):69-72
Heidarvand L,Maali-Amiri R(2013)Physio-biochemical and proteome analysis of chickpea in early phases of cold stress.J Plant Physiol 170(5):459-469
Jiang Y,Huang B(2001)Drought and heat stress injury to two cool season turfgrasses in relation to antioxidant metabolism and lipid peroxidation.Crop Sci 41:436-442
Jones KS,Paroschy J(1999)Carbohydrate composition and freezing tolerance of canes and buds in Vitis vinifera.J Plant Physiol155(1):101-106
Jouve L,Engelmann F,Noirot M,Charrier A(1993)Evaluation of biochemical markers(sugar,proline,malonedialdehyde and ethylene)for cold sensitivity in microcuttings of two coffee species.Plant Sci 91(1):109-116
Karimi R,Ershadi A(2015)Role of exogenous abscisic acid in adapting of‘Sultana’grapevine to low-temperature stress.Acta Physiol Plant 37(8):151
Karimzadeh G,Francis D,Davies MS(2000)Low temperatureinduced accumulation of protein is sustained both in root meristems and in callus in winter wheat but not in spring wheat.Ann Bot 85(6):769-777
Karimzadeh G,Sharifi-Sirchi GR,Jalali-Javaran M,Dehghani H,Francis D(2006)Soluble proteins induced by low temperature treatment in the leaves of spring and winter wheat cultivars.Pak J Bot 38(4):1015-1026
Kazemi-Shahandashti SS,Maali AR,Zeinali H,Ramezanpour SS(2013)Change in membrane fatty acid compositions and coldinduced responses in chickpea.Mol Biol Rep 40(2):893-903
Kazemi-Shahandashti SS,Maali-Amiri R,Zeinali H,Khazaei M,Talei A,Ramezanpour SS(2014)Effect of short-term cold stress on oxidative damage and transcript accumulation of defenserelated genes in chickpea seedlings.J Plant Physiol171(13):1106-1116
Koster KL,Lynch DV(1992)Solute accumulation and compartmentation during the cold acclimation of Puma Rye.Plant Physiol98(1):108-113
Li HS(2003)Principles and techniques of plant physiology and biochemistry experiment.Higher Education Press,Beijing,pp 258-260
Li J,Yan XF,Zu YG(2000)Generation of activated oxygen and change of cell defense enzyme activity in leaves of Korean pine seedling under low temperature.J For 42(2):148-152
Li CX,Shao Y,Jiang LN(2013)Biostatistics,vol 4.Science Press,Beijing,pp 85-87
Liang DY,Jin YZ,Zhao GJ,Dong YH,Leng WW,Chen CL,Wang H,Zhao XY(2016)The study on the variation of the growth and wood characters of 50 Pinus Koraiensis clones.J Beijing For Univ 38(6):51-59
Liu GF,Yang CP,Zhao GY(2002)The feasibility of the introduction of Pinus Sibirica species.Ann Meet Tree Genet Breed China For13:1483-1486
Liu YJ,Cao HX,Zhang RL(2015)Effects of low temperature stress time on physiological and biochemical changes of oil palm seedlings.Plant Res 35(06):860-865
Los DA,Mironov KS,Allakhverdiev SI(2013)Regulatory role of membrane fluidity in gene expression and physiological functions.Photosynth Res 116(2-3):489-509
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Maggio A,Miyazaki S,Veronese P,Fujita T,Ibeas JI,Damsz B,Narasimhan ML,Hasegawa PM,Joly RJ,Bressan RA(2002)Does proline accumulation play an active role in stress-induced growth reduction?Plant J 31(6):699-712
Meng P,Bai X,Li H,Song X,Zhang X(2015)Cold hardiness estimation of Pinus densiflora var.zhangwuensis based on changes in ionic leakage,chlorophyll fluorescence and other physiological activities under cold stress.J For Res26(3):641-649
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Mittova V,Volokita M,Guy M,Tal M(2000)Activities of SOD and the ascorbate-glutathione cycle enzymes in subcellular compartments in leaves and roots of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii.Physiol Plant110(1):42-51
Morin X,Ameglio T,Ahas R,Kurz-Besson C,Lanta V,Lebourgeois F,Miglietta F,Chuine I(2007)Variation in cold hardiness and carbohydrate concentration from dormancy induction to bud burst among provenances of three European oak species.Tree Physiol 27(6):817-825
Moriyama U,Tomioka R,Kojima M,Sakakibara H,Takenaka C(2016)Aluminum effect on starch,soluble sugar,and phytohormone in roots of Quercus serrata Thunb seedlings.Trees30(2):405-413
Nazari M,Amiri RM,Mehraban FH,Khaneghah HZ(2012)Change in antioxidant responses against oxidative damage in black chickpea following cold acclimation.Russ J Plant Physiol59(2):183-189
Nejadsadeghi L,Maali-Amiri R,Zeinali H,Ramezanpour S,Sadeghzade B(2014)Comparative analysis of physio-biochemical responses to cold stress in tetraploid and hexaploid wheat.Cell Biochem Biophys 70(1):399-408
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Pandey RK,Bargali SS(2017)Does seed size affect water stress tolerance in Quercus leucotrichophora A.Camus at germination and early seedling growth stage?Biodiversity International Journal 1(1):24-30
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Rakei A,Maali-Amiri R,Zeinali H,Ranjbar M(2016)DNAmethylation and physio-biochemical analysis of chickpea in response to cold stress.Protoplasma 253(1):61-76
Rivero RM,Ruiz JM,Garcia PC,Lopezlefebre LR,Sanchez E,Romero L(2002)Response of oxidative metabolism in watermelon plants subjected to cold stress.Funct Plant Biol29(5):643-648
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Bargali K,Bargali SS(2016)Germination capacity of seeds of leguminous plants under water deficit conditions:implication for restoration of degraded lands in kumaun himalaya.Trop Ecol57(3):445-453
Bertrand A,Robitaille G,Nadeau P,Castonguay Y(1999)Influence of ozone on cold acclimation in sugar maple seedlings.Tree Physiol 19(8):527-534
Bhattacharjee S(1996)Short-term heat and cold shock induced proline accumulation in relation to Ca2?involvement in Lycopersicum esculantum(Mill)cultured cells and seedlings.Indian J Plant Physiol 1(1):32-35
Bian SM,Jiang YW(2009)Reactive oxygen species,antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery.Sci Hortic 120(2):264-270
Bowler C,And MVM,Inze D(2003)Superoxide dismutase and stress tolerance.Annu Rev Plant Biol 43(1):83-116
Bradford MM(1976)A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Anal Biochem72(s1-2):248-254
Burke MJ,Gusta LV,Quamme HA,Weiser CJ,Li PH(2003)Freezing and injury in plants.Ann Rev Plant Physiol27(1):507-528
Dalen LS,Johnsen?,Lonneborg A,Yaish MW(2015)Freezing tolerance in Norway spruce,the potential role of pathogenesisrelated proteins.Acta Physiol Plant 37(1):1717
Farhangi-Abriz S,Torabian S(2017)Antioxidant enzyme and osmotic adjustment changes in bean seedlings as affected by biochar under salt stress.Ecotoxicol Environ Saf 137:64-70
Foyer CH,Noctor G(2005)Redox homeostasis and antioxidant signaling:a metabolic interface between stress perception and physiological responses.Plant Cell 17(7):1866-1875
Foyer CH,Descourvieres P,Kunert KJ(1994)Protection against oxygen radicals:an important defence mechanism studied in transgenic plants.Plant Cell Environ 17(5):507-523
Garratt LC,Janagoudar BS,Lowe KC,Anthony P,Power JB,Davey MR(2002)Salinity tolerance and antioxidant status in cotton cultures.Free Radic Biol Med 33(4):502-511
George MF,Burke MJ(1984)Supercooling of tissue water to extreme low temperature in overwintering plants.Trends Biochem Sci9(5):211-214
Gill SS,Tuteja N(2010)Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.Plant Physiol Biochem 48(12):909-930
He E,Bai RH,Shen J,Zhao FH,Cao Y(2000)Study on afforestation in the experiment of introducing Pinus Sibirica.J Inn Mong Agric Univ(Nat Sci Ed)21(2):69-72
Heidarvand L,Maali-Amiri R(2013)Physio-biochemical and proteome analysis of chickpea in early phases of cold stress.J Plant Physiol 170(5):459-469
Jiang Y,Huang B(2001)Drought and heat stress injury to two cool season turfgrasses in relation to antioxidant metabolism and lipid peroxidation.Crop Sci 41:436-442
Jones KS,Paroschy J(1999)Carbohydrate composition and freezing tolerance of canes and buds in Vitis vinifera.J Plant Physiol155(1):101-106
Jouve L,Engelmann F,Noirot M,Charrier A(1993)Evaluation of biochemical markers(sugar,proline,malonedialdehyde and ethylene)for cold sensitivity in microcuttings of two coffee species.Plant Sci 91(1):109-116
Karimi R,Ershadi A(2015)Role of exogenous abscisic acid in adapting of‘Sultana’grapevine to low-temperature stress.Acta Physiol Plant 37(8):151
Karimzadeh G,Francis D,Davies MS(2000)Low temperatureinduced accumulation of protein is sustained both in root meristems and in callus in winter wheat but not in spring wheat.Ann Bot 85(6):769-777
Karimzadeh G,Sharifi-Sirchi GR,Jalali-Javaran M,Dehghani H,Francis D(2006)Soluble proteins induced by low temperature treatment in the leaves of spring and winter wheat cultivars.Pak J Bot 38(4):1015-1026
Kazemi-Shahandashti SS,Maali AR,Zeinali H,Ramezanpour SS(2013)Change in membrane fatty acid compositions and coldinduced responses in chickpea.Mol Biol Rep 40(2):893-903
Kazemi-Shahandashti SS,Maali-Amiri R,Zeinali H,Khazaei M,Talei A,Ramezanpour SS(2014)Effect of short-term cold stress on oxidative damage and transcript accumulation of defenserelated genes in chickpea seedlings.J Plant Physiol171(13):1106-1116
Koster KL,Lynch DV(1992)Solute accumulation and compartmentation during the cold acclimation of Puma Rye.Plant Physiol98(1):108-113
Li HS(2003)Principles and techniques of plant physiology and biochemistry experiment.Higher Education Press,Beijing,pp 258-260
Li J,Yan XF,Zu YG(2000)Generation of activated oxygen and change of cell defense enzyme activity in leaves of Korean pine seedling under low temperature.J For 42(2):148-152
Li CX,Shao Y,Jiang LN(2013)Biostatistics,vol 4.Science Press,Beijing,pp 85-87
Liang DY,Jin YZ,Zhao GJ,Dong YH,Leng WW,Chen CL,Wang H,Zhao XY(2016)The study on the variation of the growth and wood characters of 50 Pinus Koraiensis clones.J Beijing For Univ 38(6):51-59
Liu GF,Yang CP,Zhao GY(2002)The feasibility of the introduction of Pinus Sibirica species.Ann Meet Tree Genet Breed China For13:1483-1486
Liu YJ,Cao HX,Zhang RL(2015)Effects of low temperature stress time on physiological and biochemical changes of oil palm seedlings.Plant Res 35(06):860-865
Los DA,Mironov KS,Allakhverdiev SI(2013)Regulatory role of membrane fluidity in gene expression and physiological functions.Photosynth Res 116(2-3):489-509
Ma JL,Zhuang LW,Chen D,Li JW(1992)The distribution of Pinus Koraiensis.J Northeast For Univ 5:40-48
Ma YY,Zhang Y,Lu JA,Shao HB(2010)Roles of plant soluble sugars and their responses to plant cold stress.Afr J Biotech8(10):2004-2010
Maggio A,Miyazaki S,Veronese P,Fujita T,Ibeas JI,Damsz B,Narasimhan ML,Hasegawa PM,Joly RJ,Bressan RA(2002)Does proline accumulation play an active role in stress-induced growth reduction?Plant J 31(6):699-712
Meng P,Bai X,Li H,Song X,Zhang X(2015)Cold hardiness estimation of Pinus densiflora var.zhangwuensis based on changes in ionic leakage,chlorophyll fluorescence and other physiological activities under cold stress.J For Res26(3):641-649
Michiels C,Raes M,Toussaint O,Remacle J(1994)Importance of SE-glutathione peroxidase,catalase,and CU/ZN-SOD for cell survival against oxidative stress.Free Radic Biol Med17(3):235-248
Mittler R(2006)Abiotic stress,the field environment and stress combination.Trends Plant Sci 11(1):15-19
Mittova V,Volokita M,Guy M,Tal M(2000)Activities of SOD and the ascorbate-glutathione cycle enzymes in subcellular compartments in leaves and roots of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennellii.Physiol Plant110(1):42-51
Morin X,Ameglio T,Ahas R,Kurz-Besson C,Lanta V,Lebourgeois F,Miglietta F,Chuine I(2007)Variation in cold hardiness and carbohydrate concentration from dormancy induction to bud burst among provenances of three European oak species.Tree Physiol 27(6):817-825
Moriyama U,Tomioka R,Kojima M,Sakakibara H,Takenaka C(2016)Aluminum effect on starch,soluble sugar,and phytohormone in roots of Quercus serrata Thunb seedlings.Trees30(2):405-413
Nazari M,Amiri RM,Mehraban FH,Khaneghah HZ(2012)Change in antioxidant responses against oxidative damage in black chickpea following cold acclimation.Russ J Plant Physiol59(2):183-189
Nejadsadeghi L,Maali-Amiri R,Zeinali H,Ramezanpour S,Sadeghzade B(2014)Comparative analysis of physio-biochemical responses to cold stress in tetraploid and hexaploid wheat.Cell Biochem Biophys 70(1):399-408
Omran RG(1980)Peroxide levels and the activities of catalase,peroxidase,and indoleacetic acid oxidase during and after chilling cucumber seedlings.Plant Physiol 65(2):407-408
Pan RC,Wang XJ,Li NH(2012)Plant physiology version 7.High Education Press,Beijing,pp 438-455
Pandey RK,Bargali SS(2017)Does seed size affect water stress tolerance in Quercus leucotrichophora A.Camus at germination and early seedling growth stage?Biodiversity International Journal 1(1):24-30
Prasad TK,Anderson MD,Martin BA,Stewart CR(1994)Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide.Plant Cell 6(1):65-74
Rai AN,Penna S(2013)Molecular evolution of plant P5CS,gene involved in proline biosynthesis.Mol Biol Rep40(11):6429-6435
Rakei A,Maali-Amiri R,Zeinali H,Ranjbar M(2016)DNAmethylation and physio-biochemical analysis of chickpea in response to cold stress.Protoplasma 253(1):61-76
Rivero RM,Ruiz JM,Garcia PC,Lopezlefebre LR,Sanchez E,Romero L(2002)Response of oxidative metabolism in watermelon plants subjected to cold stress.Funct Plant Biol29(5):643-648
Sasaki H,Ichimura K,Okada K,Oda M(1998)Freezing tolerance and soluble sugar contents affected by water stress during coldacclimation and de-acclimation in cabbage seedlings.Sci Hortic76(3):161-169
Schaberg PG,Dehayes DH(2000)Physiological and environmental causes of freezing injury in red spruce.In:Mickler RA,Birdsey RA,Hom JL(eds)Responses of Northern U.S.Forests to Environmental Change.Springer,New York,pp 181-227
Schreiber SG,Hamann A,Hacke UG,Thomas BR(2013)Sixteen years of winter stress:an assessment of cold hardiness,growth performance and survival of hybrid poplar clones at a boreal planting site.Plant Cell Environ 36(2):419-428
Senthil-Kumar M,Kumar G,Srikanthbabu V,Udayakumar M(2007)Assessment of variability in acquired thermo tolerance:potential option to study genotypic response and the relevance of stress genes.J Plant Physiol 164:111-125
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