藏药麻花秦艽不同部位提取物的镇痛和急性毒性作用比较研究
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摘要
目的比较麻花秦艽不同部位提取物的急性毒性及其镇痛作用。方法(1)甲醛致小鼠足疼痛反应:按照体重将小鼠分为6组:2个剂量实验-1组(灌胃麻花秦艽地上部分提取物10,30 g·kg~(-1)),2个剂量实验-2组(灌胃麻花秦艽地下部分提取物10,30 g·kg~(-1)),空白对照组(灌胃等量0. 5%羧甲基纤维素,CMC),阳性对照组(一次性腹腔注射吗啡30 mg·kg~(-1)),每组12只。每天2次,连续5次。末次给药后40 min,在小鼠右后足皮下注射5%甲醛30μL,观察并记录在注射后0～5 min、50～55 min的动物疼痛反应情况。(2)乙酸致小鼠扭体反应:分组与给药方法同(1)。末次末次给药后40 min,在小鼠腹腔注射0. 9%冰乙酸10μL·mg~(-1),观察并记录在注射冰乙酸后5～20 min的动物扭体次数。(3)急性毒性实验:5个剂量实验-1组分别灌胃麻花秦艽地上部分提取物72. 8,91. 2,113. 6,142. 4和177. 6 g·kg~(-1); 5个剂量实验-2组分别灌胃麻花秦艽地下部分提取物82. 0,102. 4,128. 0,160. 0和200. 0 g·kg~(-1),在给药后14 d内,观察并记录动物中毒表现和死亡情况。结果实验-1组与实验-2组对甲醛引起的2个时段的疼痛反应均无明显影响,但可抑制冰乙酸引起小鼠的扭体反应;实验-1组的LD50大于177. 6 g·kg~(-1),实验-2组的LD50为109. 80g·kg~(-1)。结论实验-1组与实验-2组均对小鼠具有一定的急性毒性作用,并有一定的镇痛作用。
Objective To observe the acesodyne activity and acute toxicity of the different fractions from Tibetan medicine of Gentiana straminea Maxim.( GSM). Methods( 1) Formaldehyde-induced pain response in mice: The mice were randomly divided into 6 groups according to their weighting: Two doses of experiment-1 groups( 10,30 g·kg~(-1) of the subsurface extract of GSM),two doses of experiment-2 groups( 10,30 g·kg~(-1) of the underground extract of GSM),blank control group( an equal amount of 0. 5% carboxymethyl cellulose,CMC),2 times a day,5 times in a row; positive control group( received a single intraperitoneal injection of morphine 30 mg·kg~(-1)),12 in each group. Forty min after the last administration,30 μL of 5% formaldehyde was injected subcutaneously into the right hind paw of the mice. The pain response of the animals during 0 min to 5 min and 50 min to 55 min after injection of formaldehyde was observed and recorded.( 2) Acetic acid-induced writhing in mice: Grouping and administration refer to( 1),12 in each group. Forty min after the last last administration,the mice were intraperitoneally injected with 0. 9% glacial acetic acid 10 μL·mg~(-1). The number of writhings of the animals during the 5 min to 20 min injection of glacial acetic acid was observed and recorded.( 3) Acute toxicity: Five doses of experiment-1 groups were administered with 72. 8,91. 2,113. 6,142. 4 and 177. 6 g·kg~(-1) of the subsurface extract of GSM; five doses of experiment-2 groups were administered with 82. 0,102. 4,128. 0,160. 0 and 200. 0 g·kg~(-1) of the underground ground extract of GSM,12 in each group. The poisoning performance and mortality of each animal within 14 d after administration were observed and recorded. Results There were no significant effect of experiment-1 groups and experiment-2 groups for 2 periods of pain caused by formaldehyde; but the GSM can inhibit the writhing response induced by glacial acetic acid in mice. The LD50 of experiment-1 group in mice is greater than 177. 6 g·kg~(-1),and the LD_(50) of experiment-2 group is 109. 80 g·kg~(-1). Conclusion All doses of GSM in the 2 groups had certain acute toxicity in mice. All doses of GSM had certain analgesic action.
Objective To observe the acesodyne activity and acute toxicity of the different fractions from Tibetan medicine of Gentiana straminea Maxim.( GSM). Methods( 1) Formaldehyde-induced pain response in mice: The mice were randomly divided into 6 groups according to their weighting: Two doses of experiment-1 groups( 10,30 g·kg~(-1) of the subsurface extract of GSM),two doses of experiment-2 groups( 10,30 g·kg~(-1) of the underground extract of GSM),blank control group( an equal amount of 0. 5% carboxymethyl cellulose,CMC),2 times a day,5 times in a row; positive control group( received a single intraperitoneal injection of morphine 30 mg·kg~(-1)),12 in each group. Forty min after the last administration,30 μL of 5% formaldehyde was injected subcutaneously into the right hind paw of the mice. The pain response of the animals during 0 min to 5 min and 50 min to 55 min after injection of formaldehyde was observed and recorded.( 2) Acetic acid-induced writhing in mice: Grouping and administration refer to( 1),12 in each group. Forty min after the last last administration,the mice were intraperitoneally injected with 0. 9% glacial acetic acid 10 μL·mg~(-1). The number of writhings of the animals during the 5 min to 20 min injection of glacial acetic acid was observed and recorded.( 3) Acute toxicity: Five doses of experiment-1 groups were administered with 72. 8,91. 2,113. 6,142. 4 and 177. 6 g·kg~(-1) of the subsurface extract of GSM; five doses of experiment-2 groups were administered with 82. 0,102. 4,128. 0,160. 0 and 200. 0 g·kg~(-1) of the underground ground extract of GSM,12 in each group. The poisoning performance and mortality of each animal within 14 d after administration were observed and recorded. Results There were no significant effect of experiment-1 groups and experiment-2 groups for 2 periods of pain caused by formaldehyde; but the GSM can inhibit the writhing response induced by glacial acetic acid in mice. The LD50 of experiment-1 group in mice is greater than 177. 6 g·kg~(-1),and the LD_(50) of experiment-2 group is 109. 80 g·kg~(-1). Conclusion All doses of GSM in the 2 groups had certain acute toxicity in mice. All doses of GSM had certain analgesic action.
引文
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[4]周金黄.中药药理[M].上海:科学技术出版社,1986:20.
[5] VOGEL H G,VOGELl W H. Drug Discovery and Evaluation:Pharmacological Assays[M]. Germany:Springer International Publishing,2015:676-677.
[6]中华人民共和国卫生部.新药(西药)临床前研究指导原则汇编[M].北京:中华人民共和国卫生部药政局,1993:121-123.
[7]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,2011:358-359.
[8]聂安政,林志健,王雨,等.秦艽化学成分及药理作用研究进展[J].中草药,2017,48(3):597-608.