黑果腺肋花楸原花青素分离纯化工艺研究
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摘要
本文以黑果腺肋花楸为原料,采用大孔树脂吸附纯化法对其中的原花青素进行分离纯化。先通过正交试验考查黑果腺肋花楸原花青素的提取工艺,确定最佳提取工艺为:70%乙醇,料液比为1∶15,pH=3,超声辅助提取30 min。通过比较5种不同大孔树脂对黑果腺肋花楸原花青素的吸附-解吸能力,筛选出DM130型大孔树脂作为最佳纯化材料。通过单因素试验确定DM130大孔树脂纯化黑果腺肋花楸原花青素的最佳分离纯化条件:上样流速为2 mL·min~(-1),上样液浓度为1 mg·mL~(-1),上样量为6BV。上样后先用蒸馏水洗脱至无色,收集40%乙醇溶液的洗脱液,所得原花青素到纯度为78.4%。
In this study, the extraction and purification of Proanthocyanidins from Aronia melanocarpa Elliot by macroporous resin was investigated. Firstly, the optimum extraction process of proanthocyanidin was obtained by orthogonal test: the pH=3, 70% ethyl alcohol, solid-liquid ratio was 1 ∶15, ultrasonic extraction time was for 30 min. By comparing the static adsorption-desorption ability of five different macroporous resins, DM130 macroporous resin was selected as the best purification material. The best purification parameters optimized by dynamic adsorption and desorption tests were as follows: flow rate was 2 mL·min~(-1); sample concentration was 1 mg·mL~(-1); sample loading amount was 6 BV. After sampling, the resin column were eluted with distilled water to be colorless, and the eluent of 40% ethanol solution was collected. The content of proanthocyanidins extracted from the eluent was 78.4% after concentrated and freeze-dried.
In this study, the extraction and purification of Proanthocyanidins from Aronia melanocarpa Elliot by macroporous resin was investigated. Firstly, the optimum extraction process of proanthocyanidin was obtained by orthogonal test: the pH=3, 70% ethyl alcohol, solid-liquid ratio was 1 ∶15, ultrasonic extraction time was for 30 min. By comparing the static adsorption-desorption ability of five different macroporous resins, DM130 macroporous resin was selected as the best purification material. The best purification parameters optimized by dynamic adsorption and desorption tests were as follows: flow rate was 2 mL·min~(-1); sample concentration was 1 mg·mL~(-1); sample loading amount was 6 BV. After sampling, the resin column were eluted with distilled water to be colorless, and the eluent of 40% ethanol solution was collected. The content of proanthocyanidins extracted from the eluent was 78.4% after concentrated and freeze-dried.
引文
[1]李翠舫,马兴华.黑果腺肋花楸形态学特征与生物学特性观察初报[J].林业科技通讯,1995(2):30-31.
[2]KIM B,KU C S,PHAM T X,et al.Aronia melanocarpa (chokeberry) polyphenol-rich extract improves antioxidant function and reduces total plasma cholesterol in apolipoprotein E knockout mice[J].Nutrition Research,2013(33):406-413.
[3]ZHANG Huiwen,YANG Yumei,MA Chaomei,et al.Structures and antioxidant and intestinal disaccharidase inhibitory activities of A-type proanthocyanidins from peanut skin[J].Journal of Agricultural and Food Chemistry,2013(37):8814-8820.
[4]骆党委,叶静,黄雅燕,等.AB-8大孔吸附树脂精制芦柑皮总黄酮及黄酮类化合物的分离[J].食品科学,2014(6):30-35.
[5]中华人民共和国卫生部.保健食品检验与评价技术规范[M].北京:中华人民共和国卫生部,2003:274-275
[6]战伟伟,司振军,王超萍,等.蓝莓叶原花青素提取工艺研究[J].粮食与油脂,2010(6):39-42.
[7]唐丽丽,刘邻渭,孙丽芳,等.大孔树脂对石榴皮中多酚物质的吸附研究[J].食品研究与开发,2011(5):48-51.
[8]安晓婷,周涛,李春阳,等.大孔树脂纯化蓝莓果渣多酚及其组成分析[J].食品科学,2013(20):1-6.
[9]王晓林,薛健飞,陈帅,等.大孔树脂法纯化锦灯笼宿萼总黄酮的工艺[J].食品科学,2014(14):58-61.
[10]冯进,李敏,曾晓雄,等.大孔树脂纯化蓝莓叶多酚及其组成分析[J].食品科学,2013(10):86-91.
[11]刘长江,栾云峰,王菲,等.大孔吸附树脂分离纯化软枣猕猴桃总黄酮[J].食品科学,2011(12):145-149.
[12]董琦,高珊,曹龙奎.大孔树脂纯化桦褐孔菌多酚及其成分分析[J].食品科学,2015(22):131-136.
[2]KIM B,KU C S,PHAM T X,et al.Aronia melanocarpa (chokeberry) polyphenol-rich extract improves antioxidant function and reduces total plasma cholesterol in apolipoprotein E knockout mice[J].Nutrition Research,2013(33):406-413.
[3]ZHANG Huiwen,YANG Yumei,MA Chaomei,et al.Structures and antioxidant and intestinal disaccharidase inhibitory activities of A-type proanthocyanidins from peanut skin[J].Journal of Agricultural and Food Chemistry,2013(37):8814-8820.
[4]骆党委,叶静,黄雅燕,等.AB-8大孔吸附树脂精制芦柑皮总黄酮及黄酮类化合物的分离[J].食品科学,2014(6):30-35.
[5]中华人民共和国卫生部.保健食品检验与评价技术规范[M].北京:中华人民共和国卫生部,2003:274-275
[6]战伟伟,司振军,王超萍,等.蓝莓叶原花青素提取工艺研究[J].粮食与油脂,2010(6):39-42.
[7]唐丽丽,刘邻渭,孙丽芳,等.大孔树脂对石榴皮中多酚物质的吸附研究[J].食品研究与开发,2011(5):48-51.
[8]安晓婷,周涛,李春阳,等.大孔树脂纯化蓝莓果渣多酚及其组成分析[J].食品科学,2013(20):1-6.
[9]王晓林,薛健飞,陈帅,等.大孔树脂法纯化锦灯笼宿萼总黄酮的工艺[J].食品科学,2014(14):58-61.
[10]冯进,李敏,曾晓雄,等.大孔树脂纯化蓝莓叶多酚及其组成分析[J].食品科学,2013(10):86-91.
[11]刘长江,栾云峰,王菲,等.大孔吸附树脂分离纯化软枣猕猴桃总黄酮[J].食品科学,2011(12):145-149.
[12]董琦,高珊,曹龙奎.大孔树脂纯化桦褐孔菌多酚及其成分分析[J].食品科学,2015(22):131-136.