摘要
本文以黑果腺肋花楸为原料,采用大孔树脂吸附纯化法对其中的原花青素进行分离纯化。先通过正交试验考查黑果腺肋花楸原花青素的提取工艺,确定最佳提取工艺为:70%乙醇,料液比为1∶15,pH=3,超声辅助提取30 min。通过比较5种不同大孔树脂对黑果腺肋花楸原花青素的吸附-解吸能力,筛选出DM130型大孔树脂作为最佳纯化材料。通过单因素试验确定DM130大孔树脂纯化黑果腺肋花楸原花青素的最佳分离纯化条件:上样流速为2 mL·min~(-1),上样液浓度为1 mg·mL~(-1),上样量为6BV。上样后先用蒸馏水洗脱至无色,收集40%乙醇溶液的洗脱液,所得原花青素到纯度为78.4%。
In this study, the extraction and purification of Proanthocyanidins from Aronia melanocarpa Elliot by macroporous resin was investigated. Firstly, the optimum extraction process of proanthocyanidin was obtained by orthogonal test: the pH=3, 70% ethyl alcohol, solid-liquid ratio was 1 ∶15, ultrasonic extraction time was for 30 min. By comparing the static adsorption-desorption ability of five different macroporous resins, DM130 macroporous resin was selected as the best purification material. The best purification parameters optimized by dynamic adsorption and desorption tests were as follows: flow rate was 2 mL·min~(-1); sample concentration was 1 mg·mL~(-1); sample loading amount was 6 BV. After sampling, the resin column were eluted with distilled water to be colorless, and the eluent of 40% ethanol solution was collected. The content of proanthocyanidins extracted from the eluent was 78.4% after concentrated and freeze-dried.
引文
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