四君子汤对溃疡性结肠炎大鼠肠道菌群调节作用研究
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摘要
目的:探讨四君子汤对溃疡性结肠炎大鼠肠道菌群的调节作用。方法:制备溃疡性结肠炎大鼠模型,实验分为正常组、模型组、常乐康组和四君子汤组,分组给药7d,检测给药后大鼠肠道菌群的数量。结果:给药7d后,模型组与正常组相比较,模型组双歧杆菌及乳酸杆菌数量减少,肠杆菌及肠球菌数量上升,两者比较具有统计学差异(P<0.05)。与模型组相比较,四君子汤组及常乐康组双歧杆菌、乳酸杆菌的数量上升,而肠杆菌及肠球菌数量下降,但四君子汤组较常乐康组作用更明显,且两者比较具有统计学差异(P<0.05)。结论:四君子汤能有效调节溃疡性结肠炎大鼠肠道菌群。
Objective: To investigate the regulatory effect of Sijunzi decoction on intestinal flora in rats with ulcerative colitis.Methods: A rat model of ulcerative colitis was prepared.The experiment was divided into normal group,model group,Changlekang group and Sijunzi decoction group.The drug was administered for 7 days in groups,and the number of intestinal flora in the rats after administration was measured.Results: After 7 days of administration,the number of Bifidobacteria and Lactobacilli in the model group decreased,and the number of Enterobacter and Enterococcus increased in the model group compared with the normal group.There was a statistically significant difference between the two groups(P<0.05).Compared with the model group,the number of Bifidobacteria and Lactobacillus in the Sijunzitang group and the Changlekang group increased,while the number of Enterobacter and Enterococcus decreased.The Sijunzi decoction group was more effective than the Changlekang group,and the two were statistically different(P<0.05).Conclusion: Sijunzi decoction can effectively regulate the intestinal flora of rats with ulcerative colitis.
Objective: To investigate the regulatory effect of Sijunzi decoction on intestinal flora in rats with ulcerative colitis.Methods: A rat model of ulcerative colitis was prepared.The experiment was divided into normal group,model group,Changlekang group and Sijunzi decoction group.The drug was administered for 7 days in groups,and the number of intestinal flora in the rats after administration was measured.Results: After 7 days of administration,the number of Bifidobacteria and Lactobacilli in the model group decreased,and the number of Enterobacter and Enterococcus increased in the model group compared with the normal group.There was a statistically significant difference between the two groups(P<0.05).Compared with the model group,the number of Bifidobacteria and Lactobacillus in the Sijunzitang group and the Changlekang group increased,while the number of Enterobacter and Enterococcus decreased.The Sijunzi decoction group was more effective than the Changlekang group,and the two were statistically different(P<0.05).Conclusion: Sijunzi decoction can effectively regulate the intestinal flora of rats with ulcerative colitis.
引文
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[9]孙晓萍,侯丽娟,王晓红,等.大鼠慢性溃疡性结肠炎模型的建立及操作规范探讨[J].天津中医药,2012,29(3):270-273.
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[11]周正,马婷,冯澜,等.马齿苋多糖对溃疡性结肠炎小鼠肠道菌群及血内毒素的影响[J].中国微生态学杂志,2014,26(6):646-648.
[12]薛宇,颜玉,蒲田,等.益生菌对大鼠溃疡性结肠炎作用的实验研究[J].黑龙江医药科学,2011,34(5):7-8.
[13]许海英,颜玉,姜威,等.益生菌联合美沙拉嗪对大鼠溃疡性结肠炎Claudin-1及TLR4的影响[J].黑龙江医药科学,2016,39(4):52-54.
[14]彭继承,吴小平,卢放根.乳酸杆菌和双歧杆菌粗制代谢产物对小鼠溃疡性结肠炎的影响[J].医学临床研究,2008,25(5):786-788.
[15]王群英,陈村龙,孙勇,等.双歧杆菌对小鼠溃疡性结肠炎模型的影响[J].中国微生态学杂志,2002,14(3):126-128.
[16]柯铭清.中草药有效成分理化与药理特性[M].长沙:湖南科技出版社,1982:254.
[17]孙立群,梁金花,高月娟.探讨纳米级中药黄芪对溃疡性结肠炎大鼠肠道菌群失调的调整作用[J].中国中医急症,2012,21(8):1263-1265.
[18]尚保敏.美沙拉嗪联合酪酸梭菌二联活菌散治疗溃疡性结肠炎的疗效观察[J].临床医学,2013,33(8):48-49.
[2]陶弘武,柳越冬.溃结安康汤对溃疡性结肠炎大鼠MDA影响的研究[J].实用中医内科杂志,2009,23(4):29-30.
[3]张盛洪,钟碧慧,陈旻湖.肠道微生态在炎症性肠病发病中的作用[J].胃肠病学,2008,13(8):508-510.
[4]Fedorak RN,Madsen KL.Probiotics and the management of inflammatory bowel disease[J].Inflamm Bowel Dis,2004,10(3):286-299.
[5]Seksik P,Rigottier-Gois L-Gramet G,et al.Alterations of the dominant faecal bacterial groups in patients with Crohn’s disease of the colon[J].Gut,2003,2(2):237-242.
[6]严梅桢,李志年,谢念祥,等.四君子汤对实验性脾虚小鼠肠道菌群的影响[J].中国微生态学杂志,1989,1(1):41-42.
[7]鞠宝玲,陆叶,朱伟,等.不同剂量四君子汤对急性肝衰竭小鼠屏障功能的影响[J].牡丹江医学院学报.2012,33(4):1-3.
[8]孟良艳,陈秀琴,石达友,等.四君子汤对脾虚大鼠肠道菌群多样性的影响[J].畜牧业医学报.2013,44(12):2029-2035.
[9]孙晓萍,侯丽娟,王晓红,等.大鼠慢性溃疡性结肠炎模型的建立及操作规范探讨[J].天津中医药,2012,29(3):270-273.
[10]韩现红,翟晓萍,戴新娟.溃疡性结肠炎相关饮食影响及管理研究现状[J].中华护理杂志,2014,49(2):216-220.
[11]周正,马婷,冯澜,等.马齿苋多糖对溃疡性结肠炎小鼠肠道菌群及血内毒素的影响[J].中国微生态学杂志,2014,26(6):646-648.
[12]薛宇,颜玉,蒲田,等.益生菌对大鼠溃疡性结肠炎作用的实验研究[J].黑龙江医药科学,2011,34(5):7-8.
[13]许海英,颜玉,姜威,等.益生菌联合美沙拉嗪对大鼠溃疡性结肠炎Claudin-1及TLR4的影响[J].黑龙江医药科学,2016,39(4):52-54.
[14]彭继承,吴小平,卢放根.乳酸杆菌和双歧杆菌粗制代谢产物对小鼠溃疡性结肠炎的影响[J].医学临床研究,2008,25(5):786-788.
[15]王群英,陈村龙,孙勇,等.双歧杆菌对小鼠溃疡性结肠炎模型的影响[J].中国微生态学杂志,2002,14(3):126-128.
[16]柯铭清.中草药有效成分理化与药理特性[M].长沙:湖南科技出版社,1982:254.
[17]孙立群,梁金花,高月娟.探讨纳米级中药黄芪对溃疡性结肠炎大鼠肠道菌群失调的调整作用[J].中国中医急症,2012,21(8):1263-1265.
[18]尚保敏.美沙拉嗪联合酪酸梭菌二联活菌散治疗溃疡性结肠炎的疗效观察[J].临床医学,2013,33(8):48-49.