PEG模拟干旱胁迫下外源生长调节剂对大豆生理生化指标的影响
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摘要
研究了经外源生长调节剂(IAA)处理的大豆[Glycine max (Linn.) Merr.)]幼苗在PEG-6000模拟干旱胁迫下的生理生化的变化。结果表明,干旱胁迫可以显著降低叶绿素a、叶绿素b和总叶绿素含量,IAA处理则显著提高叶绿素a、叶绿素b和总叶绿素含量。干旱胁迫显著增加MDA含量,IAA处理则显著降低MDA含量。干旱胁迫显著降低As A含量,增加了GSH含量;IAA处理也显著增加GSH含量。干旱胁迫时,大豆体内CAT、SOD、POD、As A-POD活性均升高,增强了大豆的抗氧化能力。IAA处理则显著增强了SOD、POD、As A-POD活性。IAA处理可以增强大豆幼苗对干旱胁迫的抵抗能力。
Several physiological and biochemical indexes changes of soybean seedlings treated with exogenous auxin(IAA)under PEG-6000 simulated drought stress were studied. The results showed that drought stress could significantly reduce the contents of chlorophyll a, chlorophyll b and total chlorophyll, while IAA could significantly increase the contents of chlorophyll a, chlorophyll b and total chlorophyll. Drought stress significantly increased MDA content, while IAA significantly decreased MDA content. Drought stress significantly decreased AsA content and increased GSH content; IAA also significantly increased GSH content. Under drought stress, the activities of CAT, SOD, POD and AsA-POD of soybean were increased,and the antioxidant capacity of soybean was enhanced. IAA significantly increased SOD, POD and AsA-POD activity. It is concluded that IAA treatment could enhance the resistance of soybean seedlings to drought stress.
Several physiological and biochemical indexes changes of soybean seedlings treated with exogenous auxin(IAA)under PEG-6000 simulated drought stress were studied. The results showed that drought stress could significantly reduce the contents of chlorophyll a, chlorophyll b and total chlorophyll, while IAA could significantly increase the contents of chlorophyll a, chlorophyll b and total chlorophyll. Drought stress significantly increased MDA content, while IAA significantly decreased MDA content. Drought stress significantly decreased AsA content and increased GSH content; IAA also significantly increased GSH content. Under drought stress, the activities of CAT, SOD, POD and AsA-POD of soybean were increased,and the antioxidant capacity of soybean was enhanced. IAA significantly increased SOD, POD and AsA-POD activity. It is concluded that IAA treatment could enhance the resistance of soybean seedlings to drought stress.
引文
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[19]杜琳,张荃.植物谷胱甘肽与抗氧化胁迫[J].山东科学,2008,21(2):27-32.
[2]李伟,曹坤芳.干旱胁迫对不同光环境下的三叶漆幼苗光合特性和叶绿素荧光参数的影响[J].西北植物学报,2006,26(2):266-275.
[3] ASADA K. Production and action of active oxygen in photosynthetic tissue[M].Boca Raton:CRC press,1994.
[4] MOLLER I M,JENSEN P E,HANSSON A. Oxidative modifications to cellular components in plants[J].Annual review of plant biology,2007,58:459-481.
[5]高蕾,刘丽君,董守坤,等.干旱胁迫对大豆幼苗叶片生理生化特性的影响[J].东北农业大学学报,2009,40(8):1-4.
[6]蒲伟凤,李桂兰,张敏,等.干旱胁迫对野生和栽培大豆根系特征及生理指标的影响[J].大豆科学,2010,29(4):615-622.
[7]董兴月,林浩,刘丽君,等.干旱胁迫对大豆生理指标的影响[J].大豆科学,2011,30(1):83-88.
[8]纪展波,蒲伟凤,李桂兰,等.野生大豆、半野生大豆和栽培大豆对苗期干旱胁迫的生理反应[J].大豆科学,2012,31(4):598-604.
[9]吕剑,喻景权.植物生长素的作用机制[J].植物生理学通讯,2004,40(5):624-628.
[10]张会灵,吴正景,郭大龙,等.IAA和SA对盐胁迫下萝卜幼苗生理特性的影响[J].北方园艺,2017(8):32-34.
[11]李亚萍,彭燕.IAA改善PEG处理下白三叶幼苗叶片抗氧化保护和渗透调节能力[J].草业科学,2017,34(11):2295-2302.
[12]孔祥生,易现峰.植物生理学实验技术[M].北京:中国农业出版社,2008.
[13]徐惠风,刘兴土,金研铭,等.向日葵叶片叶绿素和比叶重及其产量研究田[J].农业系统科学与综合研究,2003,19(2):94-97.
[14] HUSEYNOVA I M. Photosynthetic characteristics and enzymatic antioxidant capacity of leaves from wheat cultivars exposed to drought[J].Biochim biophys acta,2012,1817:1516-1523.
[15]杜润峰,郝文芳,王龙飞.达乌里胡枝子抗氧化保护系统及膜脂过氧化对干旱胁迫及复水的动态响应[J].草业学报,2012,21(2):51-61.
[16] TOTH S Z,SCHANSKER G,GARAB G. The physiological roles and metabolism of ascorbate in chloroplasts[J].Physiol plant,2013,148:161-175.
[17] FOTOPOULOS V,KANELLIS A K. Altered apoplastic ascorbate redox state in tobacco plants via ascorbate oxidase overexpression results in delayed dark-induced senescence in detached leaves[J].Plant physiol biochem,2013,73:154-160.
[18] ZETTERSTROM R,EIJKMAN C,SIR HOPLINS F G. The dawn of vitamins and other essential nutritional growth factors[J].Acta paediatrica,2006,95(11):1331-1333.
[19]杜琳,张荃.植物谷胱甘肽与抗氧化胁迫[J].山东科学,2008,21(2):27-32.