藏药五味甘露药浴颗粒对AA模型大鼠的药效作用研究
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摘要
目的:观察藏药五味甘露药浴颗粒对AA模型大鼠的药效作用。方法:除正常组大鼠外,采用弗氏完全佐剂造模,随机分为模型组、地塞米松组(0. 150 mg/kg)、五味甘露药浴颗粒高剂量组(11. 80 g/L)、中剂量组(5. 90 g/L)、低剂量组(2. 95 g/L),药浴组40℃药水浴30 min/d,模型组和正常组40℃温水浴30 min/d。给药四周,采用致炎后足容积测量、测定大鼠胸腺、脾脏脏器系数、HE染色法观察致炎侧足踝关节滑膜病理改变,评价藏药五味甘露药浴颗粒对AA大鼠的治疗效果。结果:与模型组比较,五味甘露药浴颗粒高剂量组、中剂量组、低剂量组AA大鼠原发性足肿胀度均有减轻,滑膜细胞增生、炎细胞浸润、关节软骨破坏均有不同程度的改善。结论:经藏药浴五味甘露药浴颗粒治疗后,原发性足肿胀有明显下降的趋势,踝关节病理形态有所改善,大鼠的胸腺、脾脏系数有升高的趋势,提示该药可以明显减轻关节炎症程度,增强机体的免疫功能,对类风湿关节具有抗炎消肿的治疗作用。
Objective To observe the efficacy of Tibetan medicine-bath Wuwei-Ganlu-Yaoyu-Keli( WGYK) on AA rats.Methods Except for the control group,the rat model was made by Freund's complete adjuvant. The rats were randomly divided into adjuvant arthritis group( AA),dexamethasone acetate group( DMA,0. 150 mg/Kg),high dose Wuwei-Ganlu-Yaoyu-Keli group( WGYKh,11. 80 G/L),moderate dose Wuwei-Ganlu-Yaoyu-Keli group( WGYKm,5. 90 G/L),and low dose Wuwei-Ganlu-Yaoyu-Keli group( WGYKl,2. 95 G/L). In the Tibetan medicine-bath group,the bath temperature was 40 ℃ and bathed 30 min/d. The control group and the AA model group used 40 ℃ warm water and and bathed 30 min/d too. After four weeks,by Measured of phlogistic foot volume and the coefficient of the thymus and spleen organs of rats,and used HE staining to observe the pathological changes of the synovium of the ankle joints on the inflammatory side,we evaluated the therapeutic effect of Tibetan medicine-bath on AA rats. Results Compared with the AA model group,the primary foot swelling degree of the WGYKh group,the WGYKm group and the WGYKl group was reduced,and the synovial cell proliferation,the inflammatory cell infiltration and the articular cartilage damage were improved to some extent in different degrees. Conclusion After the treatment of the Tibetan medicine-bath WGYK,the primary foot swelling was obviously decreased,the pathological morphology of the ankle joint was improved,the thymus and spleen coefficient of the rats increased,suggesting that the drug could obviously reduce the degree of inflammation of joint and enhance the immune function of the body,and have the effect of anti inflammation and reduce swelling of the rheumatoid joints.
Objective To observe the efficacy of Tibetan medicine-bath Wuwei-Ganlu-Yaoyu-Keli( WGYK) on AA rats.Methods Except for the control group,the rat model was made by Freund's complete adjuvant. The rats were randomly divided into adjuvant arthritis group( AA),dexamethasone acetate group( DMA,0. 150 mg/Kg),high dose Wuwei-Ganlu-Yaoyu-Keli group( WGYKh,11. 80 G/L),moderate dose Wuwei-Ganlu-Yaoyu-Keli group( WGYKm,5. 90 G/L),and low dose Wuwei-Ganlu-Yaoyu-Keli group( WGYKl,2. 95 G/L). In the Tibetan medicine-bath group,the bath temperature was 40 ℃ and bathed 30 min/d. The control group and the AA model group used 40 ℃ warm water and and bathed 30 min/d too. After four weeks,by Measured of phlogistic foot volume and the coefficient of the thymus and spleen organs of rats,and used HE staining to observe the pathological changes of the synovium of the ankle joints on the inflammatory side,we evaluated the therapeutic effect of Tibetan medicine-bath on AA rats. Results Compared with the AA model group,the primary foot swelling degree of the WGYKh group,the WGYKm group and the WGYKl group was reduced,and the synovial cell proliferation,the inflammatory cell infiltration and the articular cartilage damage were improved to some extent in different degrees. Conclusion After the treatment of the Tibetan medicine-bath WGYK,the primary foot swelling was obviously decreased,the pathological morphology of the ankle joint was improved,the thymus and spleen coefficient of the rats increased,suggesting that the drug could obviously reduce the degree of inflammation of joint and enhance the immune function of the body,and have the effect of anti inflammation and reduce swelling of the rheumatoid joints.
引文
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[16]帝玛尔·丹增彭措.晶珠本草[M].上海:上海科学技术出版社,2012:213.
[17]桑罗却佩著.藏医药选编[M].李多美译.西宁:青海人民出版社,2009:105.
[2]周小梅,陈秋红,多杰,等.金诃甘露药浴颗粒的抗炎作用[J].山西中医,2003,19(1):50-52.
[3]Zhao SL,Geng PL,Sang J.Immune function of rheumatoid arthritis treated by medicated-bath therapy in Tibetan medicine[J].Zhongguo Zhong Xi Yi Jie He Za Zhi 1993(13):452-453.
[4]澈力格尔,包哈申.五味甘露药浴疗法临床应用进展[J].中国民族医药杂志,2015,(1):68.
[5]冯欣.藏药五味甘露药浴现代研究的现状与预测[J].中国藏学,2014(2):168.
[6]陈奇.中药药理研究方法学[M].北京:人民卫生出版社,1993:357
[7]Yan C,Yujing W,Di W,et al.Therapeutic effects of TACI-Ig on rats with adjuvant-induced arthritis via attenuating inflammatory responses[J].Rheumatology,2011,50(5):862-870.
[8]李培培,解国雄,宋珊珊,等.大鼠佐剂性关节炎模型表现特征及评价指标[J].中国免疫学杂志,2012,28(5):453-457.
[9]McInnes IB,Schett G.The Pathogenesis of Rheumatoid Arthritis[J].New England Journal of Medicine,2011,365(23):2205-2219.
[10]温博,曾升平.类风湿关节炎的研究进展[J].世界中西医结合杂志,2014,9(9):1014-1016,1019.
[11]Scott D L.Advances in the medical management of rheumatoid arthritis[J].Hospital Medicine,2002,63(5):294-297.
[12]Infante R G.New disease-modifying and anti-inflammatory drugs[J].Geriatrics,2000,55(3):30-40.
[13]土旦次仁.中国医学百科全书·藏医学[M].上海:上海科技出版社,1999.
[14]央嘎.藏药“五味甘露药浴”的渊源之考证[C].//第四届中医药现代化国际科技大会,2013.
[15]宇妥·元丹贡布著.四部医典[M].李永年译.北京:人民卫生出版社,1983:58-409.
[16]帝玛尔·丹增彭措.晶珠本草[M].上海:上海科学技术出版社,2012:213.
[17]桑罗却佩著.藏医药选编[M].李多美译.西宁:青海人民出版社,2009:105.