酸蚀纳米钛合金种植体的早期骨整合效果
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摘要
目的:体内评估用酸蚀法构建的纳米钛合金种植体的旋转扭力和骨功能基因的表达情况。方法:钛合金Ti6Al4V种植体分为对照组(机械切割钛合金种植体)和纳米钛组(酸蚀纳米钛合金种植体)。常规将钛合金种植体植入新西兰兔子的胫骨,植入后4周,采用数字扭力仪测试钛合金种植体的旋转扭力值,采用钙黄素染色观测种植体周围的新骨生长情况,并且通过实时定量PCR检测骨功能基因核心结合因子(Runx2)、I型胶原(Col-1)和骨钙素(OCN)的表达水平。结果:纳米钛合金种植体的旋转扭力显著大于机械切割钛合金种植体(P<0.05),荧光染色显示纳米钛合金种植体周围有更多的骨组织长入,并且纳米种植体可以提高Runx2、Col-I和OCN基因的表达(P<0.05)。结论:酸蚀法在钛合金表面形成的纳米结构有利于种植体的早期骨整合,在种植材料领域有一定的应用前景。
Objective: To evaluate the removal torque and bone gene expression of nano textured Ti6Al4V implants in vivo. Methods: The implants were divided as group of nano textured Ti6Al4V implants by etching and as the control of the machined implants. All the implants were placed in the tibia of rabbits. After four weeks, removal torque was analyzed by a digital torque gauge. New bone was found with fluorescence microscope. The gene expression of Runx2, Col-1 and OCN was examined using real-time PCR. Results: Nano textured Ti6 Al4 V implants by etching showed significantly bigger removal torque(P<0.05) and higher Runx2, Col-1 and OCN gene expression(P<0.05) than the machined implants. In addition, more new bone was found around nano textured Ti6Al4V implant around. Conclusion: The nano textured Ti6Al4V implants by etching could improve the early osseointegration processin vivo, which may be applied clinically in the future.
Objective: To evaluate the removal torque and bone gene expression of nano textured Ti6Al4V implants in vivo. Methods: The implants were divided as group of nano textured Ti6Al4V implants by etching and as the control of the machined implants. All the implants were placed in the tibia of rabbits. After four weeks, removal torque was analyzed by a digital torque gauge. New bone was found with fluorescence microscope. The gene expression of Runx2, Col-1 and OCN was examined using real-time PCR. Results: Nano textured Ti6 Al4 V implants by etching showed significantly bigger removal torque(P<0.05) and higher Runx2, Col-1 and OCN gene expression(P<0.05) than the machined implants. In addition, more new bone was found around nano textured Ti6Al4V implant around. Conclusion: The nano textured Ti6Al4V implants by etching could improve the early osseointegration processin vivo, which may be applied clinically in the future.
引文
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[2]JIANG N,GUO Z,SUN D,et al.Promoting osseointegration of Ti implantsthrough micro/nanoscaled hierarchical Ti phosphate/Ti oxide hybrid coating[J].ACS Nano,2018,12(8):7883-7891.
[3]李莺,李长义.钛种植体表面改性策略及对骨整合的影响[J].中国组织工程研究,2013,17(29):5395-5402.
[4]JONáSOVáL,MüLLER F A,HELEBRANT A,et al.Biomimetic apatite formation on chemically treated titanium[J].Biomaterials,2004,25(7-8):1187-1194.
[5]卢燃,陈溯,张振庭.钛表面改性提高种植体亲水性的研究进展[J].口腔颌面修复学杂志,2017,18(3):173-176.
[6]ZHAO G,RAINES A L,WIELAND M,et al.Requirement for both micron-and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography[J].Biomaterials,2007,28(18):2821-2829.
[7]KIM J J,JEONG Y H,CHOE H C.Phenomena of nanoand micro-pore formation on Ti-(10~50)Ta alloys by plasma electrolytic oxidation for dental implants[J].J Nanosci Nanotechnol,2017,17(4):2285-290.
[8]OLIVEIRA P T,NANCI A.Nanotexturing of titanium-based surfaces upregulates expression of bonesialoprotein and osteopontin by cultured osteogenic cells[J].Biomaterials,2004,25(3):403-413.
[9]BJURSTEN L M,RASMUSSON L,OH S,et al.Titanium dioxide nanotubes enhance bone bonding in vivo[J].JBiomed Mater Res A,2010,92:1218-1224.
[10]NANCI A,WUEST J D,PERU L,et al.Chemical modification of titanium surfaces for covalent attachment of biological molecules[J].J Biomed Mater Res,1998,40(2):324-335.
[11]BUENO RDE B,ADACHI P,CASTRO-RAUCCI LM,et al.Oxidative nanopatterning of titanium surfaces promotes production and extracellular accumulation of osteopontin[J].Braz Dent J,2011,22(3):179-184.
[12]TAVARES M G,DE OLIVEIRA P T,NANCI A,et al.Treatment of a commercial,machined surface titanium implant with H2SO4/H2O2 enhances contact osteogenesis[J].Clin Oral Implants Res,2007,18(4):452-458.
[13]YIN C,ZHANG Y,CAI Q,et al.Effects of the micro-nano surface topography of titanium alloy on the biological responses of osteoblast[J].J Biomed Mater Res A,2017,105(3):757-769.
[14]李红彩,马壮.TiO2纳米管对种植体周细胞影响的研究进展[J].中国组织工程研究,2016,20(52):7899-7904.