Resistance Selection Against Abamectin in Tetranychus cinnabarinus(Boisduval) and Changes in Its Detoxification Enzyme Activity
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摘要
The susceptible strain of Tetranychus cinnabarinus(Boisduval) was collected from the cassava germplasm resource garden of Guangxi South Subtropical Agricultural Science Research Institute and raised for 15 generations, during which resistance selection against avermectin was performed through the indoor spraying of avermectin, thereby obtaining an avermectin-resistance strain(Ab-R) with a resistance ratio of 3.25. After the indoor resistance selection, the determination of detoxification enzyme activity in the Ab-R and SS strains showed that the specific activity of carboxylesterases(CarE), glutathione-s-transferases(GSTs) and multi-functional oxidase(MFO) in Ab-R_(15) was 1.27, 1.69 and 1.92 times of that in SS, respectively. In addition, the specific activity of MFO in Ab-R_5, Ab-R_(10) and Ab-R_(15) was significantly different from that in SS; there was no significant difference in the specific activity of CarE compared with SS when screened to the F_(10) generation; and when screened to the F5 generation, there was no significant difference in the specific activity of GSTs compared with SS. The results showed that the significant increase in MFO activity was an important cause of resistance to avermectin in T. cinnabarinus, and CarE and GSTs were also involved in the formation of avermectin-resistant strains.
The susceptible strain of Tetranychus cinnabarinus(Boisduval) was collected from the cassava germplasm resource garden of Guangxi South Subtropical Agricultural Science Research Institute and raised for 15 generations, during which resistance selection against avermectin was performed through the indoor spraying of avermectin, thereby obtaining an avermectin-resistance strain(Ab-R) with a resistance ratio of 3.25. After the indoor resistance selection, the determination of detoxification enzyme activity in the Ab-R and SS strains showed that the specific activity of carboxylesterases(CarE), glutathione-s-transferases(GSTs) and multi-functional oxidase(MFO) in Ab-R_(15) was 1.27, 1.69 and 1.92 times of that in SS, respectively. In addition, the specific activity of MFO in Ab-R_5, Ab-R_(10) and Ab-R_(15) was significantly different from that in SS; there was no significant difference in the specific activity of CarE compared with SS when screened to the F_(10) generation; and when screened to the F5 generation, there was no significant difference in the specific activity of GSTs compared with SS. The results showed that the significant increase in MFO activity was an important cause of resistance to avermectin in T. cinnabarinus, and CarE and GSTs were also involved in the formation of avermectin-resistant strains.
The susceptible strain of Tetranychus cinnabarinus(Boisduval) was collected from the cassava germplasm resource garden of Guangxi South Subtropical Agricultural Science Research Institute and raised for 15 generations, during which resistance selection against avermectin was performed through the indoor spraying of avermectin, thereby obtaining an avermectin-resistance strain(Ab-R) with a resistance ratio of 3.25. After the indoor resistance selection, the determination of detoxification enzyme activity in the Ab-R and SS strains showed that the specific activity of carboxylesterases(CarE), glutathione-s-transferases(GSTs) and multi-functional oxidase(MFO) in Ab-R_(15) was 1.27, 1.69 and 1.92 times of that in SS, respectively. In addition, the specific activity of MFO in Ab-R_5, Ab-R_(10) and Ab-R_(15) was significantly different from that in SS; there was no significant difference in the specific activity of CarE compared with SS when screened to the F_(10) generation; and when screened to the F5 generation, there was no significant difference in the specific activity of GSTs compared with SS. The results showed that the significant increase in MFO activity was an important cause of resistance to avermectin in T. cinnabarinus, and CarE and GSTs were also involved in the formation of avermectin-resistant strains.
引文
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[12]RU Y,CHEN YN,SHANG SQ,et al.Effect of sublethal dose of avermectin on the activities of detoxifying enzymes in Tetranychus urticae[J].Journal of Gansu Agricultural University,2017,52(1):87-91.(in Chinese)
[13]LI P,LIU LJ,LI HR,et al.Indoor resistance selection against 1.8%avermectin in Tetranychus cinnabarinus(Boisduval)[J].China Tropical Agriculture,2016,(5):46-48.(in Chinese)
[14]VAN AK.A study of housefly esterase by means of a sensitive colorinmetric method[J].J Inset Physiol,1962,8(2):401-416.
[15]CLARK AG,DICK GL,SMITH JN.Kinetic studies on a glutathione S-transferase from the larvae of Costelytra zealandica[J].B iochem,1984,217:51-58.
[16]ZHANG XY,HE J.Selection of drug resistance against avermectin B1and cross resistance in Plutella xylostella[J].Journal of Plant Protection,2011,28(2):163-168.(in Chinese)
[17]GAO XJ,ZHANG ZG,DUAN XL,et al.Resistance selection against clofentezine in Tetranychus urticae(Koch)and change of its detoxification enzymes activity[J].Scientia Agricultura Sinica,2012,45(7):1433-1438.(in Chinese)
[18]LIU JX,HAN JC,LIU HP,et al.Study on resistance mechanisms of the Hawthorn spider mite,Tetranychus viennensis Zacher,to isocarbophos and fenpropathrin[J].Journal of Sichuan University:Natural Science Edition,2006,43(6):1364-1368.(in Chinese)
[2]HE L,ZHAO ZM,DENG XP,et al.Resistance selection of Tetranychus cinnabarinus to three acaricides and lts management strategy[J].Scientia Agricultura Sinica,2003,36(4):403-408.(in Chinese)
[3]CAO XF,HE L,ZHAO ZM,et al.Study on esterase isozyme of different resistance strain in Tetranychus cinnabarinus(Boisduval)[J].Acta Arachnologica Sinica,2004,13(4):95-102.(in Chinese)()(Continued from page 16)
[4]MA ZQ.Study on the relationship between the poisoning symptoms and action mechanism of different pesticides[D].Yangling:Northwest Agriculture&Forestry University,2002.(in Chinese)
[5]SHOOP WL,MROZIK H,FISHER MH.Structure and activity of avermectins and milbemycins in animal health[J].Veterinry Parasitology,1995,59(2):139-156.
[6]CHEN WB,SUN L,YANG T,et al.Study on pesticide resistance mechanisms and resistant fitness of Tetranychus turkestani in abamectin and pyridaben[J].Xinjiang Agricultural Sciences,2011,48(2):229-235.(in Chinese)
[7]LIU YC,WANG L,ZHANG YJ,et al.Abamectin resistance and expression of resistance-related genes in field population of Tetranychus urticae(Acari:Tetranychidae)[J].Acta Entomologica Sinica,2016,59(11):1199-1205.(in Chinese)
[8]SONG LW,LI MW,SHEN HM.Study on resistance selection against pyridaben,avermectin and abamectin·pyridaben in Tetranychus truncatus and its resistance stability[J].Chinese Bulletin of Entomology,2016,53(1):89-94.(in Chinese)
[9]HE L,TAN SL,CAO XF,et al.Study on resistance selection and activity of detoxification enzyme in Tetranychus cinnabarinus(boiduval)[J].Chinese Journal of Pesticide Science,2003,5(4):23-29.(in Chinese)
[10]CLAUDIANOS C,RANSON H,JOHNSON RM,et al.A deficit of detoxification enzymes:pesticide sensitivity and environmental response in the honeybee[J].Insect Molecular Biology,2006,15(5):615-636.
[11]SHEN YF,SHEN HM,YUE XL,et al.Resistance selection of Tetranychus urticae to abamectin strain and changes in the activity of detoxification enzymes[J].Plant Protection,2014,40(5):44-48.(in Chinese)
[12]RU Y,CHEN YN,SHANG SQ,et al.Effect of sublethal dose of avermectin on the activities of detoxifying enzymes in Tetranychus urticae[J].Journal of Gansu Agricultural University,2017,52(1):87-91.(in Chinese)
[13]LI P,LIU LJ,LI HR,et al.Indoor resistance selection against 1.8%avermectin in Tetranychus cinnabarinus(Boisduval)[J].China Tropical Agriculture,2016,(5):46-48.(in Chinese)
[14]VAN AK.A study of housefly esterase by means of a sensitive colorinmetric method[J].J Inset Physiol,1962,8(2):401-416.
[15]CLARK AG,DICK GL,SMITH JN.Kinetic studies on a glutathione S-transferase from the larvae of Costelytra zealandica[J].B iochem,1984,217:51-58.
[16]ZHANG XY,HE J.Selection of drug resistance against avermectin B1and cross resistance in Plutella xylostella[J].Journal of Plant Protection,2011,28(2):163-168.(in Chinese)
[17]GAO XJ,ZHANG ZG,DUAN XL,et al.Resistance selection against clofentezine in Tetranychus urticae(Koch)and change of its detoxification enzymes activity[J].Scientia Agricultura Sinica,2012,45(7):1433-1438.(in Chinese)
[18]LIU JX,HAN JC,LIU HP,et al.Study on resistance mechanisms of the Hawthorn spider mite,Tetranychus viennensis Zacher,to isocarbophos and fenpropathrin[J].Journal of Sichuan University:Natural Science Edition,2006,43(6):1364-1368.(in Chinese)