TiO_2纳米管尺寸对其生物活性的影响
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摘要
以丙三醇为电解液体系制备出高有序的TiO2纳米管,通过模拟人体体液(SBF)溶液浸泡和体外细胞实验探讨不同尺寸TiO2纳米管生物活性的差异。利用扫描电子显微镜(SEM)、X射线衍射(XRD)等对样品进行表征。结果表明,相同电压下,随着阳极氧化电压的增加,TiO2纳米管管长和管径呈增加趋势。通过羟基磷灰石(HA)的沉积情况、MG-63细胞粘附情况、MTT检测分析,可以得出,随TiO2纳米管管长和管径的增加,诱导HA沉积能力和促进细胞粘附增殖能力增强,表现出较好的生物活性。
High order TiO2 nanotubes was prepared in glycerol solution as electrolyte by anodic oxidation.The differences of biological activity were studied of the different sizes of TiO2 nanotubes.The samples were soaked in simulated body fluid(SBF) to observe the deposition of hydroxyapatite and explored the biocompatibility by cell cytotoxicity experiments in vitro.Experimental results showed TiO2 nanotubes had different diameter and tube length under different anodic oxidation voltage.With anodizing voltage increasing the nanotubes showed increasing wall and diameter.The samples were characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD),etc.Hydrophilicity deposition,MG63 cell adhesion,and other biological evaluation were observed.The conclusion showed the samples showed the better biological activity with the increasing nanotube length and diameter of TiO2 nanotubes.
High order TiO2 nanotubes was prepared in glycerol solution as electrolyte by anodic oxidation.The differences of biological activity were studied of the different sizes of TiO2 nanotubes.The samples were soaked in simulated body fluid(SBF) to observe the deposition of hydroxyapatite and explored the biocompatibility by cell cytotoxicity experiments in vitro.Experimental results showed TiO2 nanotubes had different diameter and tube length under different anodic oxidation voltage.With anodizing voltage increasing the nanotubes showed increasing wall and diameter.The samples were characterized by scanning electron microscopy(SEM),X-ray diffraction(XRD),etc.Hydrophilicity deposition,MG63 cell adhesion,and other biological evaluation were observed.The conclusion showed the samples showed the better biological activity with the increasing nanotube length and diameter of TiO2 nanotubes.
引文
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[3]Zhao Xiaobing,Liu Xuanyong,You Jing,et al.Bioactivi-ty and cytocompatibility of plasma-sprayed titania coatingtreated by sulfuric acid treatment[J].Surface&CoatingsTechnology,2008,202:3221-3226.
[4]Wen H B,Wolke J G C,de Wijn J R,et al.Fast precipita-tion of calcium phosphate layers on titanium induced bysimple chemical treatments[J].Biomaterials,1997,18(22):1471-1478.
[5]Wen C E,Xu W,Hu W Y,et al.Hydroxyapatite/titaniasol-gel coatings on titanium-zirconium alloy for biomedicalapplications[J].Acta Biomaterialia,2007,3(3):403-410.
[6]Kim H M,Miyaji F,Kokubo T.Bonding strength ofbonelike apatite layer to Ti metal substrate[J].J BiomedMater Res,1997,38:121-127.
[7]Wen H B,de Wijn J R,Cui F Z,et al.Preparation ofbioactive microporous titanium surface by a new two-stepchemical treatment[J].J Mater Sci:Mater Med,1998,9:121-128.
[8]Ma Qiqi,Li Meiheng,Hu Ziying,et al.Enhancement ofthe bioactivity of titanium oxide nanotubes by precalcifica-tion[J].Materials Letters,2008,62(9):3035-3038.
[9]Shirkhanzadeh M.Calcium phosphate coatings preparedby electrocrystallization from aqueous electrolytes[J].JMater Sci:Mater Med,1995,6:90-93.
[10]Kim S E,Lim J H,Lee S C,et al.Anodically nano-structured titanium oxides for implant applications[J].Electrochimica Acta,2008,53:4846-4851.
[11]Gong Dawei,Grimes C A,Vargheseet O K,et al.Tita-nium oxide nanotube arrays prepared by anodic oxidation[J].J Mater Res,2001,16(12):3331-3334.
[12]Ishizawa H,Ogino M.Characterization of thin hydroxyapa-tite layers formed on anodic titanium oxide films containingCa and P by hydrothermal treatment[J].Journal of Biomed-ical Materials Research,1995,29(9):1071-1079.
[13]Li Mingou,Xiao Xiufeng,Liu Rongfang.Synthesis andbioactivity of highly ordered TiO2 nanotube arrays[J].Applied Surface Science,2008,255(6):365-367.
[14]Brammer K S,Oh S,Cobb C J,et al.Improved bone-forming functionality on diameter-controlled TiO2nano-tube surface[J].Acta Biomaterialia,2009,5(3):3215-3223.
[15]Lai Yuekun,Huang Yongxia,Lin Changjian,et al.Se-lective formation of ordered arrays of octacalcium phos-phate ribbons on TiO2nanotube surface by template-as-sisted electrodeposition[J].Colloids and Surfaces B:Biointerfaces,2010,76(10):117-122.
[16]Zhang Zhijuan,Xie Yibing,Fu Degang,et al.Covalent-ly immobilized biosensor based on gold nanoparticlesmodified TiO2nanotube arrays[J].Journal of Electroa-nalytical Chemistry,2011,650:241-247.
[17]Wan Jun,Yan Xia,Hu Kongcheng,et al.Self-organ-ized highly ordered TiO2 nanotubes in organic aqueoussystem[J].Materials Characterization,2009,60:1534-1540.
[18]Wang Xiaohong,Cao Yang,Yang Liang,et al.Bioactivi-ty study of the titanium plates treated with microarc oxi-dation and alkali[J].J Nanosci Nanotechnol,2011,11:9650-9655.
[19]Wang Yueqin,Tao Jie,Wang Ling,et al.HA coatingon titanium with nanotubular anodized TiO2intermediatelayer via electrochemical deposition[J].Trans Nonfer-rous Met Soc China,2008,18:631-635.