Intestinal toxicity of deoxynivalenol is limited by supplementation with Lactobacillus plantarum JM113 and consequentially altered gut microbiota in broiler chickens
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摘要
Background: Limited research has focused on the effect of Lactobacillus on the intestinal toxicity of deoxynivalenol(DON).The present study was conducted to investigate the role of Lactobacillus plantarum(L.plantarum) JM113 in protecting against the intestinal toxicity caused by DON.Methods: A total of 144 one-day-old healthy Arbor Acres broilers were randomly distributed into 3 treatments,including the CON(basal diet),the DON(extra 10 mg/kg deoxynivalenol),and the DL(extra 1 × 109 CFU/kg L.plantarum JM113 based on DON group) treatments.The growth performance,organ indexes,intestinal morphology,pancreatic digestive enzymes,intestinal secreted immunoglobulin A(sIgA),jejunal transcriptome,and intestinal microbiota were evaluated.Results: Compared with the CON and DL groups,the DON supplementation altered intestinal morphology,especially in duodenum and jejunum,where villi were shorter and crypts were deeper(P < 0.05).Meanwhile,the significantly decreased mRNA expression of jejunal claudin-1 and occludin(P < 0.05),ileal rBAT and jejunal GLUT1 of 21-day-old broilers(P < 0.05),as well as duodenal PepT1 and ileal rBAT of 42-day-old broilers were identified in the DON group.Moreover,supplementation with L.plantarum JM113 could increase duodenal expression of IL-10 and IL-12 of 21-dayold broilers,ileal s IgA of 42-day-old broilers,and the bursa of Fabricius index of 21-day-old broilers.Further jejunal transcriptome proved that the genes related to the intestinal absorption and metabolism were significantly reduced in the DON group but a significant increase when supplemented with extra L.plantarum JM113.Furthermore,the bacteria related to nutrient utilization,including the Proteobacteria,Escherichia,Cc-115(P < 0.05),Lactobacillus and Prevotella(P < 0.1) were all decreased in the DON group.By contrast,supplementation with L.plantarum JM113 increased the relative abundance of beneficial bacterium,including the Bacteroidetes,Roseburia,Anaerofustis,Anaerostipe,and Ruminococcus bromi(P < 0.05).Specifically,the increased abundance of bacteria in the DL group could be proved by the significantly increased caecal content of propionic acid,n-Butyric acid,and total short-chain fatty acid.Conclusions: L.plantarum JM113 enhanced the digestion,absorption,and metabolic functions of the gut when challenged with DON by reducing the injury to intestinal barriers and by increasing the abundance of beneficial bacterium.
Background: Limited research has focused on the effect of Lactobacillus on the intestinal toxicity of deoxynivalenol(DON).The present study was conducted to investigate the role of Lactobacillus plantarum(L.plantarum) JM113 in protecting against the intestinal toxicity caused by DON.Methods: A total of 144 one-day-old healthy Arbor Acres broilers were randomly distributed into 3 treatments,including the CON(basal diet),the DON(extra 10 mg/kg deoxynivalenol),and the DL(extra 1 × 109 CFU/kg L.plantarum JM113 based on DON group) treatments.The growth performance,organ indexes,intestinal morphology,pancreatic digestive enzymes,intestinal secreted immunoglobulin A(sIgA),jejunal transcriptome,and intestinal microbiota were evaluated.Results: Compared with the CON and DL groups,the DON supplementation altered intestinal morphology,especially in duodenum and jejunum,where villi were shorter and crypts were deeper(P < 0.05).Meanwhile,the significantly decreased mRNA expression of jejunal claudin-1 and occludin(P < 0.05),ileal rBAT and jejunal GLUT1 of 21-day-old broilers(P < 0.05),as well as duodenal PepT1 and ileal rBAT of 42-day-old broilers were identified in the DON group.Moreover,supplementation with L.plantarum JM113 could increase duodenal expression of IL-10 and IL-12 of 21-dayold broilers,ileal s IgA of 42-day-old broilers,and the bursa of Fabricius index of 21-day-old broilers.Further jejunal transcriptome proved that the genes related to the intestinal absorption and metabolism were significantly reduced in the DON group but a significant increase when supplemented with extra L.plantarum JM113.Furthermore,the bacteria related to nutrient utilization,including the Proteobacteria,Escherichia,Cc-115(P < 0.05),Lactobacillus and Prevotella(P < 0.1) were all decreased in the DON group.By contrast,supplementation with L.plantarum JM113 increased the relative abundance of beneficial bacterium,including the Bacteroidetes,Roseburia,Anaerofustis,Anaerostipe,and Ruminococcus bromi(P < 0.05).Specifically,the increased abundance of bacteria in the DL group could be proved by the significantly increased caecal content of propionic acid,n-Butyric acid,and total short-chain fatty acid.Conclusions: L.plantarum JM113 enhanced the digestion,absorption,and metabolic functions of the gut when challenged with DON by reducing the injury to intestinal barriers and by increasing the abundance of beneficial bacterium.
Background: Limited research has focused on the effect of Lactobacillus on the intestinal toxicity of deoxynivalenol(DON).The present study was conducted to investigate the role of Lactobacillus plantarum(L.plantarum) JM113 in protecting against the intestinal toxicity caused by DON.Methods: A total of 144 one-day-old healthy Arbor Acres broilers were randomly distributed into 3 treatments,including the CON(basal diet),the DON(extra 10 mg/kg deoxynivalenol),and the DL(extra 1 × 109 CFU/kg L.plantarum JM113 based on DON group) treatments.The growth performance,organ indexes,intestinal morphology,pancreatic digestive enzymes,intestinal secreted immunoglobulin A(sIgA),jejunal transcriptome,and intestinal microbiota were evaluated.Results: Compared with the CON and DL groups,the DON supplementation altered intestinal morphology,especially in duodenum and jejunum,where villi were shorter and crypts were deeper(P < 0.05).Meanwhile,the significantly decreased mRNA expression of jejunal claudin-1 and occludin(P < 0.05),ileal rBAT and jejunal GLUT1 of 21-day-old broilers(P < 0.05),as well as duodenal PepT1 and ileal rBAT of 42-day-old broilers were identified in the DON group.Moreover,supplementation with L.plantarum JM113 could increase duodenal expression of IL-10 and IL-12 of 21-dayold broilers,ileal s IgA of 42-day-old broilers,and the bursa of Fabricius index of 21-day-old broilers.Further jejunal transcriptome proved that the genes related to the intestinal absorption and metabolism were significantly reduced in the DON group but a significant increase when supplemented with extra L.plantarum JM113.Furthermore,the bacteria related to nutrient utilization,including the Proteobacteria,Escherichia,Cc-115(P < 0.05),Lactobacillus and Prevotella(P < 0.1) were all decreased in the DON group.By contrast,supplementation with L.plantarum JM113 increased the relative abundance of beneficial bacterium,including the Bacteroidetes,Roseburia,Anaerofustis,Anaerostipe,and Ruminococcus bromi(P < 0.05).Specifically,the increased abundance of bacteria in the DL group could be proved by the significantly increased caecal content of propionic acid,n-Butyric acid,and total short-chain fatty acid.Conclusions: L.plantarum JM113 enhanced the digestion,absorption,and metabolic functions of the gut when challenged with DON by reducing the injury to intestinal barriers and by increasing the abundance of beneficial bacterium.
引文
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2.Tian Y,Tan Y,Liu N,Liao Y,Sun C,Wang S,et al.Functional agents to biologically control deoxynivalenol contamination in cereal grains.Front Microbiol.2016;7:1-8.https://doi.org/10.3389/fmicb.2016.00395.
3.Awad WA,Aschenbach JR,Setyabudi F,Razzazi-Fazeli E,B?hm J,Zentek J.In vitro effects of deoxynivalenol on small intestinal d-glucose uptake and absorption of deoxynivalenol across the isolated jejunal epithelium of laying hens.Poult Sci.2007;86:15-20.
4.Weaver AC,See MT,Kim SW.Protective effect of two yeast based feed additives on pigs chronically exposed to deoxynivalenol and zearalenone.Toxins.2014;6:3336-53.
5.Awad WA,Zentek J.The feed contaminant deoxynivalenol affects the intestinal barrier permeability through inhibition of protein synthesis.Arch Toxicol.2015;89:961-5.
6.Payros D,Alassane-Kpembi I,Pierron A,Loiseau N,Pinton P,Oswald IP.Toxicology of deoxynivalenol and its acetylated and modified forms.Arch Toxicol.2016;90:2931-57.
7.Pinton P,Oswald IP.Effect of deoxynivalenol and other type Btrichothecenes on the intestine:a review.Toxins.2014;6:1615-43.
8.Pestka JJ.Deoxynivalenol:mechanisms of action,human exposure,and toxicological relevance.Arch Toxicol.2010a;84:663-79.
9.Pestka JJ.Deoxynivalenol-induced proinflammatory gene expression:mechanisms and pathological sequelae.Toxins.2010b;2:1300-17.
10.Awad WA,Vahjen W,Aschenbach JR,Zentek J.A diet naturally contaminated with the fusarium mycotoxin deoxynivalenol down regulates gene expression of glucose transporters in the intestine of broiler chickens.Livestock Sci.2011;140:72-9.
11.Broekaert N,Devreese M,van Bergen T,Schauvliege S,De Boevre M,De Saeger S,et al.In vivo contribution of deoxynivalenol-3-β-D-glucoside to deoxynivalenol exposure in broiler chickens and pigs:oral bioavailability,hydrolysis and toxicokinetics.Arch Toxicol.2017;91:699-712.
12.Donohoe DR,Garge N,Zhang X,Sun W,O'Connell TM,Bunger MK,et al.The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon.Cell Metab.2011;13:517-26.
13.Yang L,Liu S,Ding J,Dai R,He C,Xu K,et al.Gut microbiota co-microevolution with selection for host humoral immunity.Front Microbiol.2017;8:1243.https://doi.org/10.3389/fmicb.2017.01243.
14.Round JL,Mazmanian SK.The gut microbiota shapes intestinal immune responses during health and disease.Nat Rev Immunol.2009;9:313-23.
15.Tremaroli V,B?ckhed F.Functional interactions between the gut microbiota and host metabolism.Nature.2012;489:242-9.
16.De Filippo C,Cavalieri D,Di Paola M,Ramazzotti M,Poullet JB,Massart S,et al.Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa.Proc Natl Acad Sci U S A.2010;(33):14691-6.
17.Jeong HG,Kang MJ,Kim HG,Oh DG,Kim JS,Lee SK,et al.Role of intestinal microflora in xenobiotic-induced toxicity.Mol Nutr Food Res.2013;57:84-99.
18.Robert H,Payros D,Pinton P,Théodorou V,Mercier-Bonin M,Oswald IP.Impact of mycotoxins on the intestine:are mucus and microbiota new targets?J Toxicol Environ Health B.2017;20:249-75.
19.Liao Y,Peng Z,Chen L,Nüssler AK,Liu L,Yang W.Deoxynivalenol,gut microbiota and immunotoxicity:a potential approach?Food Chem Toxicol.2018;112:342-54.
20.Weaver AC,See MT,Hansen JA,Kim YB,De Souza AL,Middleton TF,Kim SW.The use of feed additives to reduce the effects of aflatoxin and deoxynivalenol on pig growth,organ health and immune status during chronic exposure.Toxins.2013;5:1261-81.
21.Gallo A,Giuberti G,Frisvad JC,Bertuzzi T,Nielsen KF.Review on mycotoxin issues in ruminants:occurrence in forages,effects of mycotoxin ingestion on health status and animal performance and practical strategies to counteract their negative effects.Toxins.2015;7:3057-111.
22.Yang X,Li L,Duan Y,Yang X.Antioxidant activity of Lactobacillus plantarum JM113 in vitro and its protective effect on broiler chickens challenged with deoxynivalenol.J Anim Sci.2017;95:837-46.
23.Brisbin JT,Gong J,Orouji S,Esufali J,Mallick AI,Parvizi P,et al.Oral treatment of chickens with lactobacilli influences elicitation of immune responses.Clin Vaccine Immunol.2011;18:1447-55.
24.Feng J,Liu P,Yang X,Zhao X.Screening of immunomodulatory and adhesive lactobacillus with antagonistic activities against Salmonella from fermented vegetables.World J Microb Biot.2015;31:1947-54.
25.Naghi Shokri A,Ghasemi HA,Taherpour K.Evaluation of Aloe vera and synbiotic as antibiotic growth promoter substitutions on performance,gut morphology,immune responses and blood constitutes of broiler chickens.Anim Sci J.2017;88:306-13.
26.Vernon SD,Shukla SK,Conradt J,Unger ER,Reeves WC.Analysis of 16s rRNA gene sequences and circulating cell-free DNA from plasma of chronic fatigue syndrome and non-fatigued subjects.BMC Microbiol.2002;2:39.
27.Mago?T,Salzberg SL.FLASH:fast length adjustment of short reads to improve genome assemblies.Bioinformatics.2011;27:2957-63.
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