五指毛桃药材中补骨脂素、佛手柑内酯与芹菜素的含量测定及质量评价
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摘要
目的旨在测定五指毛桃药材中补骨脂素、佛手柑内酯和芹菜素3种成分含量,以评价不同产地、不同采收期五指毛桃药材的质量差异。方法采用高效液相色谱(HPLC)法,对17批五指毛桃药材中补骨脂素、佛手柑内酯和芹菜素进行含量测定。色谱条件:Diamonsil ODS C_(18)色谱柱(250 mm×4. 6 mm,5μm),流动相为甲醇-0.1%甲酸水溶液(55∶45,V/V),检测波长为268 nm,流速为1.0 m L·min~(-1),柱温:为30℃。结果不同产地、不同采收期的五指毛桃中3种成分的含量差异较大。其中在广东肇庆10月采收的五指毛桃中补骨脂素最高;在广东梅州大埔县1月采收的五指毛桃中佛手柑内酯含量最高;芹菜素仅在部分地区采收的五指毛桃中检测到。结论补骨脂素可作为五指毛桃药材质量评价的主要指标成分,佛手柑内酯可作为次要指标成分。本研究综合评价了广东和广西多个地区不同采收期的五指毛桃药材的质量差异,为五指毛桃的规范化种植及进一步的深度利用和开发提供依据。
OBJECTIVE Psoralen,bergamot lactone and apigenin in Radix Fici Hirtae are simultaneously determinated to evaluate the quality difference of Radix Fici Hirtae from different habitats and different harvest time. METHODS An HPLC method was used to determinate the content of psoralen,bergamot lactone and apigenin in 17 batched Radix Fici Hirtae from different habitats and different harvest time. A Diamonsil ODS C_(18) column( 250 mm× 4. 6 mm,5 μm) was used in this study. The mobile phase was methanol-0.1% formic acid aqueous solution( 55 ∶45,V/V). The detection wavelength was 268 nm,the flow rate was 1.0 mL·min~(-1),and the column temperature was 30 ℃. RESULTS The content of the three ingredients in Radix Fici Hirtae from different habitats and different harvest time was great different. The Radix Fici Hirtae collected from Zhaoqing,Guangdong in October had the highest content of psoralen.The Radix Fici Hirtae collected from Dabu Country,Meizhou,Guangdong in January had the highest content of bergamot lactone. Apigenin was only detected in Radix Fici Hirtae collected from some habitats. CONCLUSION Psoralen can be used as the main index component of quality evaluation of Radix Fici Hirtae and bergamot lactone can be used as a secondary indicator component.This experiment comprehensively evaluated the quality differences of Radix Fici Hirtae in different harvest periods in Guangdong and Guangxi,providing basis for standardized planting and further in-depth utilization and development of Radix Fici Hirtae.
OBJECTIVE Psoralen,bergamot lactone and apigenin in Radix Fici Hirtae are simultaneously determinated to evaluate the quality difference of Radix Fici Hirtae from different habitats and different harvest time. METHODS An HPLC method was used to determinate the content of psoralen,bergamot lactone and apigenin in 17 batched Radix Fici Hirtae from different habitats and different harvest time. A Diamonsil ODS C_(18) column( 250 mm× 4. 6 mm,5 μm) was used in this study. The mobile phase was methanol-0.1% formic acid aqueous solution( 55 ∶45,V/V). The detection wavelength was 268 nm,the flow rate was 1.0 mL·min~(-1),and the column temperature was 30 ℃. RESULTS The content of the three ingredients in Radix Fici Hirtae from different habitats and different harvest time was great different. The Radix Fici Hirtae collected from Zhaoqing,Guangdong in October had the highest content of psoralen.The Radix Fici Hirtae collected from Dabu Country,Meizhou,Guangdong in January had the highest content of bergamot lactone. Apigenin was only detected in Radix Fici Hirtae collected from some habitats. CONCLUSION Psoralen can be used as the main index component of quality evaluation of Radix Fici Hirtae and bergamot lactone can be used as a secondary indicator component.This experiment comprehensively evaluated the quality differences of Radix Fici Hirtae in different harvest periods in Guangdong and Guangxi,providing basis for standardized planting and further in-depth utilization and development of Radix Fici Hirtae.
引文
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[7]蔡青圆,陈虎彪,赵中振,等.五指毛桃拮抗毒品可卡因的肝毒性作用及其活性成分研究[J].中国中药杂志,2007,32(12):1190-1193.
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[11]利红宇,林志云,王成蹊,等.五指毛桃根对呼吸道和消化道的作用[J].中国现代药物应用,2008,2(17):50-51.
[12]王艳,叶木荣,周添浓,等.五指毛桃水提液保护胃黏膜及改善微循环的实验研究[J].时珍国医国药,2011,22(5):1181-1182.
[13]曾茂贵,叶华,陈学习,等.五指毛桃水提液对小鼠心、脑缺氧缺血的保护与耐缺氧能力的影响[J].福建中医药,2009,40(1):49-50.
[14]王晓平,黄翔,陈晓白,等.瑶药五指毛桃对受辐射小鼠肺组织病理形态学的影响[J].玉林师范学院学报,2010,31(5):80-83.
[15]王晓平,段丽菊,陈晓白,等.五指毛桃水提液体外抗菌作用的实验研究[J].时珍国医国药,2010,21(7):1692-1693.
[16]秦雪莲.高效液相色谱法测定不同产地五指毛桃中补骨脂素的含量[J].中国医师杂志,2006,8(2):270-271.
[17]伍世恒,黄志海,龚又明. UPLC法测定不同产地五指毛桃中补骨脂素的含量[J].新中医,2017,49(8):16-19.
[18]温玲,吴华碧,杨文豪,等.不同叶型不同采收部位五指毛桃中补骨脂素含量的测定[J].时珍国医国药,2008,19(3):687-688.
[19]陈超志,王武静,段元静,等.五指毛桃及其混淆品的鉴别研究[J].中药材,2017,40(4):797-802.
[20]杨燕军,代军. HPLC法测定五指毛桃中补骨脂素、佛手柑内酯、芹菜素的含量[J].南方医科大学学报,2010,30(11):2565-2567.
[2]广东中药志编委会.广东中药志(上)[M].广州:广东科技出版社,1994:163.
[3]江苏省植物研究.新华本草纲要(Ⅱ)[M].上海:上海科学技术出版社,1991:19.
[4]赵丽萍,狄斌,冯锋.五指毛桃的化学成分[J].药学与临床研究,2008,16(1):5-7.
[5]江滨,刘占强,曾元儿,等.五指毛桃化学成分研究[J].中草药,2005,36(8):1141-1142.
[6]轧霁,张晓琦,王英,等.五指毛桃黄酮和香豆素类成分研究[J].林产化学与工业,2008,28(6):49-52.
[7]蔡青圆,陈虎彪,赵中振,等.五指毛桃拮抗毒品可卡因的肝毒性作用及其活性成分研究[J].中国中药杂志,2007,32(12):1190-1193.
[8]吕颖坚,贾凤兰,阮明,等.五指毛桃水提物对二甲基甲酰胺所致小鼠急性肝损伤的保护作用[J].中药材,2008,31(9):1364-1368.
[9]周添浓,王艳,唐立海,等.五指毛桃抗炎镇痛及对急性肝损伤的保护作用研究[J].今日药学,2008,18(2):55-58.
[10]刘春玲,徐鸿华,吴清和,等.五指毛桃对小鼠免疫功能影响的实验研究[J].中药材,2004,27(5):367-368.
[11]利红宇,林志云,王成蹊,等.五指毛桃根对呼吸道和消化道的作用[J].中国现代药物应用,2008,2(17):50-51.
[12]王艳,叶木荣,周添浓,等.五指毛桃水提液保护胃黏膜及改善微循环的实验研究[J].时珍国医国药,2011,22(5):1181-1182.
[13]曾茂贵,叶华,陈学习,等.五指毛桃水提液对小鼠心、脑缺氧缺血的保护与耐缺氧能力的影响[J].福建中医药,2009,40(1):49-50.
[14]王晓平,黄翔,陈晓白,等.瑶药五指毛桃对受辐射小鼠肺组织病理形态学的影响[J].玉林师范学院学报,2010,31(5):80-83.
[15]王晓平,段丽菊,陈晓白,等.五指毛桃水提液体外抗菌作用的实验研究[J].时珍国医国药,2010,21(7):1692-1693.
[16]秦雪莲.高效液相色谱法测定不同产地五指毛桃中补骨脂素的含量[J].中国医师杂志,2006,8(2):270-271.
[17]伍世恒,黄志海,龚又明. UPLC法测定不同产地五指毛桃中补骨脂素的含量[J].新中医,2017,49(8):16-19.
[18]温玲,吴华碧,杨文豪,等.不同叶型不同采收部位五指毛桃中补骨脂素含量的测定[J].时珍国医国药,2008,19(3):687-688.
[19]陈超志,王武静,段元静,等.五指毛桃及其混淆品的鉴别研究[J].中药材,2017,40(4):797-802.
[20]杨燕军,代军. HPLC法测定五指毛桃中补骨脂素、佛手柑内酯、芹菜素的含量[J].南方医科大学学报,2010,30(11):2565-2567.