Expression of TAC1 and Its Receptors TACR1,TACR2,TACR3 Genes in Different Goat Tissues Under Different Photoperiods
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摘要
This study was conducted to analyze the regulation mechanisms of TAC1,TACR1,TACR2 and TACR3 genes on reproduction of goat under different photoperiods. The expression conditions of TAC1,TACR1,TACR2 and TACR3 genes in 12 tissues( oviduct,ovary,uterus,gluteus,mesenteric fat,brain,cerebellum,medulla oblongata,hart,lung,liver,kidney) of adult Henan Huai goat under different photoperiods( short day light,Light 8 h∶ dark 16 h,and long day light,light 16 h∶ dark 8 h) were analyzed by q-PCR method. TAC1,TACR1,TACR2 and TACR3 genes were expressed in all the 12 tissues of goats,with different expression characteristics; and the expression levels of all the genes were affected by photoperiod and changing of light signal between light and dark under short photoperiod. Shifting of light signal from dark to light was more conductive to the expression of these genes in all tissues than that of light signal from light to dark.There were significant differences in the expression levels of genes between light shifting from light to dark and from dark to light when the genes were expressed at a higher level in some tissues. TAC1 and TACR2 genes were expressed at a higher level than TACR1 and TACR3 genes in the various tissues,which implied that TACR2 is a receptor given priority to combine with TAC1 when TAC1 is functional.
This study was conducted to analyze the regulation mechanisms of TAC1,TACR1,TACR2 and TACR3 genes on reproduction of goat under different photoperiods. The expression conditions of TAC1,TACR1,TACR2 and TACR3 genes in 12 tissues( oviduct,ovary,uterus,gluteus,mesenteric fat,brain,cerebellum,medulla oblongata,hart,lung,liver,kidney) of adult Henan Huai goat under different photoperiods( short day light,Light 8 h∶ dark 16 h,and long day light,light 16 h∶ dark 8 h) were analyzed by q-PCR method. TAC1,TACR1,TACR2 and TACR3 genes were expressed in all the 12 tissues of goats,with different expression characteristics; and the expression levels of all the genes were affected by photoperiod and changing of light signal between light and dark under short photoperiod. Shifting of light signal from dark to light was more conductive to the expression of these genes in all tissues than that of light signal from light to dark.There were significant differences in the expression levels of genes between light shifting from light to dark and from dark to light when the genes were expressed at a higher level in some tissues. TAC1 and TACR2 genes were expressed at a higher level than TACR1 and TACR3 genes in the various tissues,which implied that TACR2 is a receptor given priority to combine with TAC1 when TAC1 is functional.
This study was conducted to analyze the regulation mechanisms of TAC1,TACR1,TACR2 and TACR3 genes on reproduction of goat under different photoperiods. The expression conditions of TAC1,TACR1,TACR2 and TACR3 genes in 12 tissues( oviduct,ovary,uterus,gluteus,mesenteric fat,brain,cerebellum,medulla oblongata,hart,lung,liver,kidney) of adult Henan Huai goat under different photoperiods( short day light,Light 8 h∶ dark 16 h,and long day light,light 16 h∶ dark 8 h) were analyzed by q-PCR method. TAC1,TACR1,TACR2 and TACR3 genes were expressed in all the 12 tissues of goats,with different expression characteristics; and the expression levels of all the genes were affected by photoperiod and changing of light signal between light and dark under short photoperiod. Shifting of light signal from dark to light was more conductive to the expression of these genes in all tissues than that of light signal from light to dark.There were significant differences in the expression levels of genes between light shifting from light to dark and from dark to light when the genes were expressed at a higher level in some tissues. TAC1 and TACR2 genes were expressed at a higher level than TACR1 and TACR3 genes in the various tissues,which implied that TACR2 is a receptor given priority to combine with TAC1 when TAC1 is functional.
引文
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[13]LOWRY PJ.Has the mechanism by which the endocrine placenta scavenges the mother whilst sparing the foetus been unmasked[J].Journal of molecular endocrinology,2003,31(3):341-347.
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[15]DEBELJUK L,RAO JN,BARTKE A.Tachykinins and their possible modulatory role on testicular function:a review[J].International journal of andrology,2003,26(4):202-210.
[16]PINTO FM,ALMEIDA TA,HERNANDEZ M,et al.mRNA expression of tachykinins and tachykinin receptors in different human tissues[J].European journal of pharmacology,2004,494(2-3):233-239.
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[18]DEBELJUK L,LASAGA M.Modulation of the hypothalamo-pituitarygonadal axis and the pineal gland by neurokinin A,neuropeptide K and neuropeptide gamma[J].Peptides,1999,20(2):285-299.
[19]SATAKE H,OGASAWARA M,KAWADA T,et al.Tachykinin and tachykinin receptor of an ascidian,Ciona intestinalis:evolutionary origin of the vertebrate tachykinin family[J].The Journal of biological chemistry,2004,279(51):53798-805.
[20]CANDENAS L,LECCI A,PINTO FM,et al.Tachykinins and tachykinin receptors:effects in the genitourinary tract[J].Life sciences,2005,76(8):835-62.
[21]RAVINA CG,SEDA M,PINTO FM,et al.A role for tachykinins in the regulation of human sperm motility[J].Hum Reprod,2007,22(6):1617-1625.
[33]PINTO FM,RAVINA CG,SUBIRAN N,et al.Autocrine regulation of human sperm motility by tachykinins[J].Reproductive Biology and Endocrinology,2010,8:104.
[22]GAO HS,HUANG F,WANG YZ,et al.the effects of hypobaric hypoxia on the changes of gene expression profile and TAC1 and MT1A in rats cerebral cortex[J].Chinese Journal of Rehabilitation Medicine,2013,28(8):699-707.(in Chinese)
[23]MIN J,LI MY,LIU YH,et al.The explore of interaction between SPAG6 and TAC1[J].National Journal of Andrology,2016,22(12):1059-1064.(in Chinese)
[24]ZHANG Y.The protective role of transient receptor potential vanilloid 1via substance P in apoptosis post acute myocardial infarction in mice[D].Chongqing:Chongqing Medical University,2015.(in Chinese)
[25]WANG XH.Effect of neurokinin A on wound healing of deep second degree burn in rats[D].Shanghai:Shanghai Jiao Tong University,2014.(in Chinese)
[38]LU Y.Study on two agonists of neurokinin receptor on colon cancer cell proliferation[D].Hanzhou:Zhejiang Sci-Tech University,2015.(in Chinese)
[26]WRANGLE J,MACHIDA EO,DANILOVA L,et al.Functional identification of cancer-specific methylation of CDOl,HOXA9,and TACl for the diagnosis of lung cancer[J].Clinical Cancer Research:An Official Journal of the American Association for Cancer Research.2014,20(7):1856-1864.
[27]MENG F,DEMORROW S,VENTER J,et al.Overexpression of membrane metalloendopeptidase inhibits substance P stimulation of cholangiocarcinoma growth[J].American Journal of Physiology Gastrointestinal and Liver Physiology,2014,306(9):G759-68.
[28]WANG QY.Domestic livestock ecology in China[M].Beijing:China Agriculture Press,2008.(in Chinese)
[29]BONDURANT RH,DARIEN BJ,MUNRO CJ,et al.Photoperiod induction of fertile oestrus and changes in LH and progesterone concentrations in yearling dairy goats(Capra hircus)[J].J Reprod Fertil,1981,63(1):1-9.
[30]WOOD SH,CHRISTIAN HC,MIEDZINSKA K,et al.Binary switching of calendar cells in the pituitary defines the phase of the circannual cycle in mammals[J].Curr Biol,2015,25(20):2651-2662.
[44]SHONA H WOOD,HELEN C CHRISTIAN,KATARZYNA MIEDZINS-KA,et al.Binary switching of calendar cells in the pituitary defines the phase of the circannual cycle in mammals[J].Curr Biol,2015,25(20):2651-2662.
[31]WEEMS PW,GOODMAN RL,LEHMAN MN.Neural mechanisms controlling seasonal reproduction:principles derived from the sheep model and its comparison with hamsters[J].Front Neuroendocrinol,2015,37:43-51.
[32]DUPRSM,MIEDZINSKA K,DUVAL CV,et al.Identification of Eya3 and TAC1 as long-day signals in the sheep pituitary[J].Curr Biol,2010,20(9):829-35.
[2]HAZBUN TR,MALMSTROM L,ANDERSON S,et al.Assigning function to yeast proteins by integration of technologies[J].Mol Cell,2003,12(6):1353-1365
[3]WESTWICK JK,MICHNICK SW.Protein-fragment complementation assays(PCA)in small GTPase research and drug discovery[J].Methods Enzymol,2005,407:388-401
[4]ZHU H,BILGIN M,BANGHAM R,et al.Global analysis of protein activities using proteome chips[J].Science,2001,293(5537):2101-2105.
[5]GIBSON SJ,BLOOM SR,POLAK JM.A novel substance P pathway linking the dorsal and ventral horn in the upper lumbar segments of the rat spinal cord[J].Brain Res,1984,301:243-51.
[6]MAGGIO JE.Tachykinins[J].Annu Rev Neurosci,1988,11:13-28.
[7]HANON EA,LINCOLN GA,FUSTIN JM,et al.Ancestral TSH mechanism signals summer in a photoperiodic mammal[J].Curr.Biol.,2008,(18):1147-1152.
[8]STIRLAND JA,JOHNSTON JD,CAGAMPANG FR,et al.Photoperiodic regulation of prolactin gene expression in the Syrian hamster by a pars tuberalis-derived factor[J].Neuroendocrinol,2001,(13):147-157.
[9]GRAHAM ES,WEBSTER CA,HAZLERIGG DG,et al.Evidence for the biosynthesis of a prolactin-releasing factor from the ovine pars tuberalis,which is distinct from thyrotropin-releasing hormone[J].Neuroendocrinol,2002,(14):945-954.
[10]PENNEFATHER JN,LECCI A,CANDENAS ML,et al.Tachykinins and tachykinin receptors a growing family[J].Life Sci,2004,(74):1445-1463
[11]TRAURIG H,SARIA A,LEMBECK F.Substance P in primary afferent neurons of the female rat reproductive system[J].Naunyn-Schmiedeberg's archives of pharmacology,1984,326(4):343-346.
[12]PENNEFATHER JN,PATAK E,PINTO FM,et al.Mammalian tachykinins and uterine smooth muscle:the challenge escalates[J].European journal of pharmacology,2004,500(1-3):15-26.
[13]LOWRY PJ.Has the mechanism by which the endocrine placenta scavenges the mother whilst sparing the foetus been unmasked[J].Journal of molecular endocrinology,2003,31(3):341-347.
[14]CHIWAKATA C,BRACKMANN B,HUNT N,et al.Tachykinin(substance-P)gene expression in Leydig cells of the human and mouse testis[J].Endocrinology,1991,128(5):2441-8.
[15]DEBELJUK L,RAO JN,BARTKE A.Tachykinins and their possible modulatory role on testicular function:a review[J].International journal of andrology,2003,26(4):202-210.
[16]PINTO FM,ALMEIDA TA,HERNANDEZ M,et al.mRNA expression of tachykinins and tachykinin receptors in different human tissues[J].European journal of pharmacology,2004,494(2-3):233-239.
[17]SASTRY BV,JANSON VE,OWENS LK.Significance of substance P-and enkephalin-peptide systems in the male genital tract[J].Annals of the New York Academy of Sciences,1991,632:339-353.
[18]DEBELJUK L,LASAGA M.Modulation of the hypothalamo-pituitarygonadal axis and the pineal gland by neurokinin A,neuropeptide K and neuropeptide gamma[J].Peptides,1999,20(2):285-299.
[19]SATAKE H,OGASAWARA M,KAWADA T,et al.Tachykinin and tachykinin receptor of an ascidian,Ciona intestinalis:evolutionary origin of the vertebrate tachykinin family[J].The Journal of biological chemistry,2004,279(51):53798-805.
[20]CANDENAS L,LECCI A,PINTO FM,et al.Tachykinins and tachykinin receptors:effects in the genitourinary tract[J].Life sciences,2005,76(8):835-62.
[21]RAVINA CG,SEDA M,PINTO FM,et al.A role for tachykinins in the regulation of human sperm motility[J].Hum Reprod,2007,22(6):1617-1625.
[33]PINTO FM,RAVINA CG,SUBIRAN N,et al.Autocrine regulation of human sperm motility by tachykinins[J].Reproductive Biology and Endocrinology,2010,8:104.
[22]GAO HS,HUANG F,WANG YZ,et al.the effects of hypobaric hypoxia on the changes of gene expression profile and TAC1 and MT1A in rats cerebral cortex[J].Chinese Journal of Rehabilitation Medicine,2013,28(8):699-707.(in Chinese)
[23]MIN J,LI MY,LIU YH,et al.The explore of interaction between SPAG6 and TAC1[J].National Journal of Andrology,2016,22(12):1059-1064.(in Chinese)
[24]ZHANG Y.The protective role of transient receptor potential vanilloid 1via substance P in apoptosis post acute myocardial infarction in mice[D].Chongqing:Chongqing Medical University,2015.(in Chinese)
[25]WANG XH.Effect of neurokinin A on wound healing of deep second degree burn in rats[D].Shanghai:Shanghai Jiao Tong University,2014.(in Chinese)
[38]LU Y.Study on two agonists of neurokinin receptor on colon cancer cell proliferation[D].Hanzhou:Zhejiang Sci-Tech University,2015.(in Chinese)
[26]WRANGLE J,MACHIDA EO,DANILOVA L,et al.Functional identification of cancer-specific methylation of CDOl,HOXA9,and TACl for the diagnosis of lung cancer[J].Clinical Cancer Research:An Official Journal of the American Association for Cancer Research.2014,20(7):1856-1864.
[27]MENG F,DEMORROW S,VENTER J,et al.Overexpression of membrane metalloendopeptidase inhibits substance P stimulation of cholangiocarcinoma growth[J].American Journal of Physiology Gastrointestinal and Liver Physiology,2014,306(9):G759-68.
[28]WANG QY.Domestic livestock ecology in China[M].Beijing:China Agriculture Press,2008.(in Chinese)
[29]BONDURANT RH,DARIEN BJ,MUNRO CJ,et al.Photoperiod induction of fertile oestrus and changes in LH and progesterone concentrations in yearling dairy goats(Capra hircus)[J].J Reprod Fertil,1981,63(1):1-9.
[30]WOOD SH,CHRISTIAN HC,MIEDZINSKA K,et al.Binary switching of calendar cells in the pituitary defines the phase of the circannual cycle in mammals[J].Curr Biol,2015,25(20):2651-2662.
[44]SHONA H WOOD,HELEN C CHRISTIAN,KATARZYNA MIEDZINS-KA,et al.Binary switching of calendar cells in the pituitary defines the phase of the circannual cycle in mammals[J].Curr Biol,2015,25(20):2651-2662.
[31]WEEMS PW,GOODMAN RL,LEHMAN MN.Neural mechanisms controlling seasonal reproduction:principles derived from the sheep model and its comparison with hamsters[J].Front Neuroendocrinol,2015,37:43-51.
[32]DUPRSM,MIEDZINSKA K,DUVAL CV,et al.Identification of Eya3 and TAC1 as long-day signals in the sheep pituitary[J].Curr Biol,2010,20(9):829-35.