PIAS3在胃癌组织中的表达及临床意义
|
摘要
目的探讨PIAS3在胃癌(GC)组织中的表达及其与临床病理及预后的关系。方法选取2006年1月～2008年5月于我院确诊的112例GC患者的癌组织和配对癌旁正常胃黏膜组织分别作为研究组和对照组。采用免疫组化染色法检测PIAS3在GC组织和癌旁正常胃黏膜组织的表达水平,并分析PIAS3蛋白在GC组织表达水平与患者各临床病理学特征及预后的关系。结果 PIAS3蛋白在GC组织中的水平低于癌旁正常胃黏膜组织,差异具有统计学意义(P<0.05);不同性别、年龄、肿瘤大小、肿瘤部位、Lauren分型的GC患者组织中PIAS3蛋白水平比较差异无统计学意义(P>0.05);肿瘤浸润程度、淋巴结转移不同的GC患者组织中PIAS3蛋白水平比较差异无统计学意义(P<0.05);PIAS3水平不同的GC患者生存曲线比较差异无统计学意义(P<0.05);PIAS3低表达的GC患者死亡风险高于高表达患者(P<0.05)。结论 PIAS3蛋白在GC组织中呈低表达,其水平与肿瘤浸润、淋巴结转移及GC预后相关。
Objective To investigate the correlation of PIAS3 expression level with clinicopathological features and prognosisin gastric cancer(GC) patients. Methods The cancer tissues(study group) and adjacent normal tissues(control group) of 112 patients with GC diagnosed in our hospital from January 2006 to May 2008 were selected. Then the expression levels of PIAS3 in both groups were detected by immunohistochemical staining, and its correlation with clinicopathological features and prognosis was also analyzed. Results The high expression rate of PIAS3 in the study group was lower than that of the control group(P<0.05). The expression level of PIAS3 was not correlated with gender, age, tumor size, tumor location and Lauren classification(P>0.05), while was correlated with tumor invasion degrees and lymph node metastasis(P<0.05). The survival curves of GC patients with different PIAS3 expression was significantly different(P<0.05). The mortality risk was significantly higher in patients with low PIAS3 expression than in patients with high PIAS3 expression(P<0.05).Conclusion The expression level of PIAS3 protein in GC tissues is relatively low, which is correlated with tumor invasion, lymph node metastasis and prognosis.
Objective To investigate the correlation of PIAS3 expression level with clinicopathological features and prognosisin gastric cancer(GC) patients. Methods The cancer tissues(study group) and adjacent normal tissues(control group) of 112 patients with GC diagnosed in our hospital from January 2006 to May 2008 were selected. Then the expression levels of PIAS3 in both groups were detected by immunohistochemical staining, and its correlation with clinicopathological features and prognosis was also analyzed. Results The high expression rate of PIAS3 in the study group was lower than that of the control group(P<0.05). The expression level of PIAS3 was not correlated with gender, age, tumor size, tumor location and Lauren classification(P>0.05), while was correlated with tumor invasion degrees and lymph node metastasis(P<0.05). The survival curves of GC patients with different PIAS3 expression was significantly different(P<0.05). The mortality risk was significantly higher in patients with low PIAS3 expression than in patients with high PIAS3 expression(P<0.05).Conclusion The expression level of PIAS3 protein in GC tissues is relatively low, which is correlated with tumor invasion, lymph node metastasis and prognosis.
引文
[1] Oliveira C,Senz J,Kaurah P,et al.Germline CDH1 deletions in hereditary diffuse gastric cancer families[J].Human Molecular Genetics,2016,18(9):1545.
[2] Wang XL,Jiang JT,Wu CP.Prognostic significance of tumor-associated macrophage infiltration in gastric cancer:a meta-analysis[J].Genetics & Molecular Research Gmr,2016,15(4).doi:10.4238/gmr15049040.
[3] 吴朝,董自强.STAT3及其抑制剂在膀胱癌中的研究进展[J].东南大学学报(医学版),2015(2):313-316.
[4] 李和平,熬建萍,杨政.PIAS3在结肠癌组织和细胞中的表达及意义[J].肿瘤学杂志,2017,23(2):106-110.
[5] 左婷婷,郑荣寿,曾红梅,等.中国胃癌流行病学现状[J].中国肿瘤临床,2017,44(1):52-58.
[6] 林莹,闫艳,吴峥,等.胃癌组织原钙黏蛋白10 mRNA表达水平与预后的相关性[J].中国癌症杂志,2017,27(1):7-13.
[7] 闫振宇,买春阳,高鹏,等.Her2在乳腺癌和胃癌中表达的临床意义[J].中国免疫学杂志,2016,32(6):858-862.
[8] 穆桂芳,穆雪峰,毕海静,等.VEGF和突变型p53在胃癌组织表达及意义[J].中国免疫学杂志,2016(1):90-91.
[9] 孙海翔,徐余超,陈晓伟,等.PI3K/AKT/mTOR信号通路相关蛋白在胃癌中表达[J].中国公共卫生,2017(10):1455-1458.
[10] 李和平,熬建萍,杨政.PIAS3在结肠癌组织和细胞中的表达及意义[J].肿瘤学杂志,2017,23(2):106-110.
[11] Li C,Li H,Zhang P,et al.SHP2,SOCS3 and PIAS3 Expression Patterns in Medulloblastomas:Relevance to STAT3 Activation and Resveratrol-Suppressed STAT3 Signaling[J].Nutrients,2017,9(1):3.
[12] Abbas R,Mccoll KS,Kresak A,et al.PIAS3 expression in squamous cell lung cancer is low and predicts overall survival[J].Cancer Medicine,2015,4(3):325-332.
[13] Campla CK,Breit H,Dong L,et al.Pias3 is necessary for dorso-ventral patterning and visual response of retinal cones but is not required for rod photoreceptor differentiation[J].Biology Open,2017,6(6):881-890.
[14] Wu CS,Zou L.The SUMO (Small Ubiquitin-like Modifier) Ligase PIAS3 Primes ATR for Checkpoint Activation[J].Journal of Biological Chemistry,2016,291(1):279.
[15] Lin X,Lin BW,Chen XL,et al.PAI-1/PIAS3/Stat3/miR-34a forms a positive feedback loop to promote EMT-mediated metastasis through Stat3 signaling in Non-small cell lung cancer[J].Biochem Biophys Res Commun,2017.
[16] Li C,Li H,Zhang P,et al.SHP2,SOCS3 and PIAS3 Expression Patterns in Medulloblastomas:Relevance to STAT3 Activation and Resveratrol-Suppressed STAT3 Signaling[J].Nutrients,2017,9(1):3.
[17] 马文玲,郭顺林,魏柯香,等.功能磁共振成像技术在肝脏的应用现状[J].磁共振成像,2017,8(11):866-870.
[18] Liu LH,Li H,Cheng XX,et al.Correlative analyses of the expression levels of PIAS3,p-SHP2,SOCS1 and SOCS3 with STAT3 activation in human astrocytomas[J].Molecular Medicine Reports,2017,15(2):847-852.
[19] 俞庆华,李峰生,黄乃祥,等.PIAS3过表达对肺腺癌细胞A549增殖的影响及意义[J].中国医学装备,2015(1):10-13.
[20] 张瑜丽,蒋奇,周阳,等.阿托伐他汀对房颤细胞模型中ATBF1/PIAS3/STAT3通路的影响[J].药物生物技术,2016(1):17-21.
[21] Lin X,Lin BW,Chen XL,et al.PAI-1/PIAS3/Stat3/miR-34a forms a positive feedback loop to promote EMT-mediated metastasis through Stat3 signaling in Non-small cell lung cancer[J].Biochem Biophys Res Commun,2017.
[2] Wang XL,Jiang JT,Wu CP.Prognostic significance of tumor-associated macrophage infiltration in gastric cancer:a meta-analysis[J].Genetics & Molecular Research Gmr,2016,15(4).doi:10.4238/gmr15049040.
[3] 吴朝,董自强.STAT3及其抑制剂在膀胱癌中的研究进展[J].东南大学学报(医学版),2015(2):313-316.
[4] 李和平,熬建萍,杨政.PIAS3在结肠癌组织和细胞中的表达及意义[J].肿瘤学杂志,2017,23(2):106-110.
[5] 左婷婷,郑荣寿,曾红梅,等.中国胃癌流行病学现状[J].中国肿瘤临床,2017,44(1):52-58.
[6] 林莹,闫艳,吴峥,等.胃癌组织原钙黏蛋白10 mRNA表达水平与预后的相关性[J].中国癌症杂志,2017,27(1):7-13.
[7] 闫振宇,买春阳,高鹏,等.Her2在乳腺癌和胃癌中表达的临床意义[J].中国免疫学杂志,2016,32(6):858-862.
[8] 穆桂芳,穆雪峰,毕海静,等.VEGF和突变型p53在胃癌组织表达及意义[J].中国免疫学杂志,2016(1):90-91.
[9] 孙海翔,徐余超,陈晓伟,等.PI3K/AKT/mTOR信号通路相关蛋白在胃癌中表达[J].中国公共卫生,2017(10):1455-1458.
[10] 李和平,熬建萍,杨政.PIAS3在结肠癌组织和细胞中的表达及意义[J].肿瘤学杂志,2017,23(2):106-110.
[11] Li C,Li H,Zhang P,et al.SHP2,SOCS3 and PIAS3 Expression Patterns in Medulloblastomas:Relevance to STAT3 Activation and Resveratrol-Suppressed STAT3 Signaling[J].Nutrients,2017,9(1):3.
[12] Abbas R,Mccoll KS,Kresak A,et al.PIAS3 expression in squamous cell lung cancer is low and predicts overall survival[J].Cancer Medicine,2015,4(3):325-332.
[13] Campla CK,Breit H,Dong L,et al.Pias3 is necessary for dorso-ventral patterning and visual response of retinal cones but is not required for rod photoreceptor differentiation[J].Biology Open,2017,6(6):881-890.
[14] Wu CS,Zou L.The SUMO (Small Ubiquitin-like Modifier) Ligase PIAS3 Primes ATR for Checkpoint Activation[J].Journal of Biological Chemistry,2016,291(1):279.
[15] Lin X,Lin BW,Chen XL,et al.PAI-1/PIAS3/Stat3/miR-34a forms a positive feedback loop to promote EMT-mediated metastasis through Stat3 signaling in Non-small cell lung cancer[J].Biochem Biophys Res Commun,2017.
[16] Li C,Li H,Zhang P,et al.SHP2,SOCS3 and PIAS3 Expression Patterns in Medulloblastomas:Relevance to STAT3 Activation and Resveratrol-Suppressed STAT3 Signaling[J].Nutrients,2017,9(1):3.
[17] 马文玲,郭顺林,魏柯香,等.功能磁共振成像技术在肝脏的应用现状[J].磁共振成像,2017,8(11):866-870.
[18] Liu LH,Li H,Cheng XX,et al.Correlative analyses of the expression levels of PIAS3,p-SHP2,SOCS1 and SOCS3 with STAT3 activation in human astrocytomas[J].Molecular Medicine Reports,2017,15(2):847-852.
[19] 俞庆华,李峰生,黄乃祥,等.PIAS3过表达对肺腺癌细胞A549增殖的影响及意义[J].中国医学装备,2015(1):10-13.
[20] 张瑜丽,蒋奇,周阳,等.阿托伐他汀对房颤细胞模型中ATBF1/PIAS3/STAT3通路的影响[J].药物生物技术,2016(1):17-21.
[21] Lin X,Lin BW,Chen XL,et al.PAI-1/PIAS3/Stat3/miR-34a forms a positive feedback loop to promote EMT-mediated metastasis through Stat3 signaling in Non-small cell lung cancer[J].Biochem Biophys Res Commun,2017.