油菜素内酯诱导小白菜初生根不对称生长
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摘要
油菜素内酯(Brassinolide,BL)能诱导单子叶植物初生根发生不对称生长,而在双子叶植物中还未见报道。本试验通过外源施加不同浓度2,4-表油菜素内酯(2,4-Epibrassinolide,2,4-eBL)观察小白菜(Brassica chinensis L.)初生根生长状况。结果表明:2,4-eBL能诱导小白菜初生根发生不对称生长形成卷曲。随着2,4-eBL浓度的升高,根卷曲比例和曲率逐渐增大,同时向地性逐渐降低。试验发现外源施加2,4-eBL可诱导双子叶植物根不对称生长的现象,为研究油菜素甾醇(Brassinosteroids,BRs)在根生长发育中的作用提供新途径。
Brassinolide(BL) can induce asymmetric growth of primary roots of monocots, but has not been reported in dicots. The text used the seedlings of Brassica chinensis L. as the experimental materials to observe the growth of primary root by exogenous application of different concentrations of 2,4-epibrassinolide(2,4-eBL). The results showed that 2,4-eBL could induce asymmetric growth of the primary root of B. chinensis L. to form curls, while the curling ratio and curvature of the root growth gradually increased. For the first time, the test found that the external application of 2,4-eBL induced the asymmetric root growth of dicotyledonous plants, and provided a new way to study the role of Brassinosteroids(BRs) in root growth and development.
Brassinolide(BL) can induce asymmetric growth of primary roots of monocots, but has not been reported in dicots. The text used the seedlings of Brassica chinensis L. as the experimental materials to observe the growth of primary root by exogenous application of different concentrations of 2,4-epibrassinolide(2,4-eBL). The results showed that 2,4-eBL could induce asymmetric growth of the primary root of B. chinensis L. to form curls, while the curling ratio and curvature of the root growth gradually increased. For the first time, the test found that the external application of 2,4-eBL induced the asymmetric root growth of dicotyledonous plants, and provided a new way to study the role of Brassinosteroids(BRs) in root growth and development.
引文
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[23]杨文,于旭东,蔡泽坪,等.接触刺激在水稻根不对称生长中的作用研究[J].热带作物学报,2018,39(9):18-23.
[24]Cai Z P,Yang W,Zhang H Y,et al.Ethylene participates in the brassinolide-regulated asymmetric growth of O.sativa root[J].South African Journal of Botany,2018(119):86-93.
[25]Karnachuk R A,Golovatskaya I F,Efimova M V,et al.The effect of epibrassinolide on Arabidopsis seedling morphogenesis and hormonal balance under green light[J].Russian Journal of Plant Physiology,2002,49(4):530-533.
[26]Mullen J L,Turk E,Johnson K,et al.Root-growth behavior of the Arabidopsis mutant rgr1.Roles of gravitropism and circumnutation in the waving/coiling phenomenon[J].Plant Physiology,1998,118(4):1139-1145.
[27]Jiang J,Li J,Xu Y,et al.RNAi knockdown of Oryza sativa root meander curling gene led to altered root development and coiling which were mediated by jasmonic acid signalling in rice[J].Plant Cell&Environment,2007,30(6):690-699.
[28]Woods S L,Roberts J A,Taylor I B.Ethylene-induced root coiling in tomato seedlings[J].Plant Growth Regulation,1984,2(3):217-225.
[29]Kim S K,Chang S C,Lee E J,et al.Involvement of brassinosteroids in the gravitropic response of primary root of maize[J].Plant Physiology,2000,123(3):997-1004.
[2]Oklestkova J,RárováL,Kvasnica M,et al.Brassinosteroids:synthesis and biological activities[J].Phytochemistry Reviews,2015,14(6):1053-1072.
[3]Fujioka S,Yokota T.Biosynthesis and metabolism of brassinosteroids[J].Physiologia Plantarum,1997,100(3):137-164.
[4]Russinova E.Plants grow on Brassinosteroids[J].Current Opinion in Plant Biology,2011,14(5):530.
[5]徐曼,齐天明,黄立涛,等.油菜素内酯对高羊茅种苗萌发生长的影响[J].生物化工,2017,3(4):17-19.
[6]刘灵,李海航,杨丽霞,等.油菜素内酯促进绿豆下胚轴不定根发生研究[J].科技导报,2007,25(5):56-60.
[7]Gupta A,Singh M,Laxmi A.Interaction between glucose and brassinosteroid during the regulation of lateral root development in Arabidopsis[J].Plant Physiology,2015,168(1):307-320.
[8]Wei Z,Li J.Brassinosteroids regulate root growth,Development,and Symbiosis[J].Molecular Plant,2016,9(1):86.
[9]Li L,Xu J,Xu Z H,et al.Brassinosteroids stimulate plant tropisms through modulation of polar auxin transport in Brassica and Arabidopsis[J].Plant Cell,2005,17(10):2738.
[10]Müssig C,Shin G H,Altmann T.Brassinosteroids promote root growth in Arabidopsis[J].Plant Physiology,2003,133(3):1261-1271.
[11]Yokota T,Sato T,Takeuchi Y,et al.Roots and shoots of tomato produce 6-deoxo-28-norcathasterone,6-deoxo-28-nortyphasterol and 6-deoxo-28-norcastasterone,possible precursors of 28-norcastasterone[J].Phytochemistry,2001,58(2):233-238.
[12]Nomura T,Bishop G J,Koncz C,et al.Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis[J].Plant Physiology,2002,130(1):504-513.
[13]Shimada Y,Goda H,Nakamura A,et al.Organ-specific expression of brassinosteroid-biosynthetic genes and distribution of endogenous brassinosteroids in Arabidopsis[J].Plant Physiology,2003,131(1):287-297.
[14]Vragovi?K,Sela A,Friedlander-Shani L,et al.Translatome analyses capture of opposing tissue-specific brassinosteroid signals orchestrating root meristem differentiation[J].Proceedings of the National Academy of Sciences of the United States of America,2015,112(3):923-928.
[15]Roddick J G,Guan M.Brassinosteroids and root development[C]//ACS Symposium Series,1991(474):231-245.
[16]Sasse J M.Recent progress in brassinosteroid research[J].Physiologia Plantarum,1997,100(3):696-701.
[17]Konishi H,Komatsu S.A proteomics approach to investigating promotive effects of brassinolide on lamina inclination and root growth in rice seedlings[J].Biological&Pharmaceutical Bulletin,2003,26(4):401-408.
[18]Hacham Y,Holland N,Butterfield C,et al.Brassinosteroid perception in the epidermis controls root meristem size[J].Development,2011,138(5):839-848.
[19]Soochul S K,Eunjoo L,Woosook C,et al.Involvement of brassinosteroids in the gravitropic response of primary root of maize[J].Plant Physiology,2000,123(3):997-1004.
[20]Kim T W,Lee S M,Joo S H,et al.Elongation and gravitropic responses of Arabidopsis roots are regulated by brassinolide and IAA[J].Plant Cell&Environment,2007,30(6):679-689.
[21]李焜章,李学真.双子叶植物和单子叶植物的基本区别[J].生物学通报,1988(1):18-19.
[22]杨文,蔡泽坪,于旭东,等.生长素抑制油菜素内酯导致的水稻根不对称生长[J].热带作物学报,2018,39(7):1304-1310.
[23]杨文,于旭东,蔡泽坪,等.接触刺激在水稻根不对称生长中的作用研究[J].热带作物学报,2018,39(9):18-23.
[24]Cai Z P,Yang W,Zhang H Y,et al.Ethylene participates in the brassinolide-regulated asymmetric growth of O.sativa root[J].South African Journal of Botany,2018(119):86-93.
[25]Karnachuk R A,Golovatskaya I F,Efimova M V,et al.The effect of epibrassinolide on Arabidopsis seedling morphogenesis and hormonal balance under green light[J].Russian Journal of Plant Physiology,2002,49(4):530-533.
[26]Mullen J L,Turk E,Johnson K,et al.Root-growth behavior of the Arabidopsis mutant rgr1.Roles of gravitropism and circumnutation in the waving/coiling phenomenon[J].Plant Physiology,1998,118(4):1139-1145.
[27]Jiang J,Li J,Xu Y,et al.RNAi knockdown of Oryza sativa root meander curling gene led to altered root development and coiling which were mediated by jasmonic acid signalling in rice[J].Plant Cell&Environment,2007,30(6):690-699.
[28]Woods S L,Roberts J A,Taylor I B.Ethylene-induced root coiling in tomato seedlings[J].Plant Growth Regulation,1984,2(3):217-225.
[29]Kim S K,Chang S C,Lee E J,et al.Involvement of brassinosteroids in the gravitropic response of primary root of maize[J].Plant Physiology,2000,123(3):997-1004.