乳腺癌患者血清性激素、IGF-1、VEGF和组织LOX水平与淋巴结转移的相关性分析
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摘要
目的分析乳腺癌患者血清性激素、胰岛素样生长因子1(IGF-1)、血管内皮生长因子(VEGF)和组织赖氨酰化酶(LOX)水平与淋巴结转移的相关性。方法回顾性分析2015年2月至2017年12月我院86例行手术治疗的乳腺癌患者临床资料,其中伴淋巴结转移者44例(转移组),无淋巴结转移者42例(无转移组),并将同期入院的40例乳腺良性肿瘤患者纳入良性组。记录3组患者术前血清雌二醇(E2)、睾酮(T)、孕酮(P)、IGF-1、VEGF水平及组织(乳腺癌患者取乳腺癌组织、良性组取良性病变组织)LOX相对表达量差异,并分析乳腺癌患者血清E2、T、P水平、组织LOX相对表达量及IGF-1、VEGF水平与淋巴结转移的相关性。结果 3组术前血清T水平比较,差异无统计学意义(P>0.05);而血清E2、P、IGF-1、VEGF水平及组织LOX相对表达量比较,均为转移组高于无转移组及良性组(P<0.05),且无转移组高于良性组(P<0.05)。术前,无转移组血清IGF-1、VEGF水平低于转移组(P<0.05);术后4周时,2组血清IGF-1、VEGF水平均较术前降低(P<0.05),且无转移组仍低于转移组(P<0.05)。经Spearman相关性分析发现,乳腺癌患者血清T及P水平与其淋巴结转移无相关性(r=-0.049、0.162,P>0.05),而血清E2、IGF-1、VEGF水平、乳腺癌组织LOX相对表达量与其淋巴结转移呈显著正相关(r=0.825、0.865、0.791、0.750,P<0.05)。结论乳腺癌患者术前血清E2、IGF-1、VEGF水平及组织LOX相对表达量与淋巴结转移状态呈正相关,临床可将血清E2、IGF-1、VEGF水平结合乳腺癌组织LOX表达情况作为预测乳腺癌患者预后的指标。
Objective To analyze the correlation between serum sex hormones levels,insulin-like growth factor 1(IGF-1), vascular endothelial growth factor(VEGF), tissue lysyloxidase(LOX)levels and lymph node metastasis in patients with breast cancer. Methods The clinical data of 86 patients with breast cancer who underwent surgery in our hospital from February 2015 to December 2017 were retrospectively analyzed,including 44 patients with lymph node metastasis(metastasis group)and 42 patients without lymph node metastasis(non-metastasis group). And another 40 patients with benign breast tumors admitted to the hospital in the same period were included in benign group. The levels of serum estradiol(E2),testosterone(T),progesterone(P),IGF-1 and VEGF and relative expression level of tissue(breast cancer tissues from patients with breast cancer,benign lesions tissues from benign group)LOX before operation were recorded in the 3 groups. The correlation between serum E2,T and P levels,relative expression level of tissue LOX,IGF-1 and VEGF levels and lymph node metastasis was analyzed. Results There was no significant difference in serum T level among the 3 groups(P>0.05). The levels of serum E2,P,IGF-1 and VEGF and the relative expression level of tissue LOX in metastasis group were higher than those in non-metastasis group and benign group(P<0.05),and the indexes in non-metastasis group were higher than those in benign group(P<0.05).Before operation,the levels of serum IGF-1 and VEGF in non-metastasis group were lower than those in metastasis group(P<0.05). At 4 weeks after operation,the levels of serum IGF-1 and VEGF in the 2 groups were lower than those before operation(P<0.05),and the levels in non-metastasis group were still lower than those in metastasis group(P<0.05). Spearman correlation analysis found that serum T and P levels were not correlated with lymph node metastasis in patients with breast cancer(r=-0.049,0.162,P>0.05)while serum E2,IGF-1 and VEGF levels and relative expression level of breast cancer tissue LOX were positively correlated with lymph node metastasis(r=0.825,0.865,0.791,0.750,P<0.05). Conclusions The preoperative levels of serum E2,IGF-1 and VEGF and relative expression level of tissue LOX in patients with breast cancer are positively correlated with lymph node metastasis. The serum levels of E2,IGF-1 and VEGF combined with expression level of breast cancer tissue LOX can be used as an index to predict the prognosis of patients with breast cancer.
Objective To analyze the correlation between serum sex hormones levels,insulin-like growth factor 1(IGF-1), vascular endothelial growth factor(VEGF), tissue lysyloxidase(LOX)levels and lymph node metastasis in patients with breast cancer. Methods The clinical data of 86 patients with breast cancer who underwent surgery in our hospital from February 2015 to December 2017 were retrospectively analyzed,including 44 patients with lymph node metastasis(metastasis group)and 42 patients without lymph node metastasis(non-metastasis group). And another 40 patients with benign breast tumors admitted to the hospital in the same period were included in benign group. The levels of serum estradiol(E2),testosterone(T),progesterone(P),IGF-1 and VEGF and relative expression level of tissue(breast cancer tissues from patients with breast cancer,benign lesions tissues from benign group)LOX before operation were recorded in the 3 groups. The correlation between serum E2,T and P levels,relative expression level of tissue LOX,IGF-1 and VEGF levels and lymph node metastasis was analyzed. Results There was no significant difference in serum T level among the 3 groups(P>0.05). The levels of serum E2,P,IGF-1 and VEGF and the relative expression level of tissue LOX in metastasis group were higher than those in non-metastasis group and benign group(P<0.05),and the indexes in non-metastasis group were higher than those in benign group(P<0.05).Before operation,the levels of serum IGF-1 and VEGF in non-metastasis group were lower than those in metastasis group(P<0.05). At 4 weeks after operation,the levels of serum IGF-1 and VEGF in the 2 groups were lower than those before operation(P<0.05),and the levels in non-metastasis group were still lower than those in metastasis group(P<0.05). Spearman correlation analysis found that serum T and P levels were not correlated with lymph node metastasis in patients with breast cancer(r=-0.049,0.162,P>0.05)while serum E2,IGF-1 and VEGF levels and relative expression level of breast cancer tissue LOX were positively correlated with lymph node metastasis(r=0.825,0.865,0.791,0.750,P<0.05). Conclusions The preoperative levels of serum E2,IGF-1 and VEGF and relative expression level of tissue LOX in patients with breast cancer are positively correlated with lymph node metastasis. The serum levels of E2,IGF-1 and VEGF combined with expression level of breast cancer tissue LOX can be used as an index to predict the prognosis of patients with breast cancer.
引文
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[15]凌锐,周玲,周月鹏,等.Wnt1诱导分泌蛋白-1通过NF-κB通路促进食管鳞癌细胞的增殖和侵袭转移[J].江苏大学学报(医学版),2018,28(2):129-134.
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[18]张敏敏,韦薇,刘剑仑,等.乳腺癌组织LOX蛋白表达与腋窝淋巴结转移相关性分析[J].中华肿瘤防治杂志,2017,24(4):236-241.
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[3]陈金辉,焦喜林,吴劲松,等.雌、孕激素受体表达状态与局部晚期乳腺癌经内乳动脉置管新辅助化疗疗效的关系[J].解放军医药杂志,2017,29(2):74-77.
[4]Cox TR,Gartland A,Erler JT.Lysyl Oxidase,a targetable secreted molecule involved in cancer metastasis[J].Cancer Research,2016,76(2):188-192.
[5]S?awomir?,Zajkowska M,Edyta KG,et al.Plasma levels and diagnostic utility of VEGF,MMP-2 and TIMP-2 in the diagnostics of breast cancer patients[J].Biomarkers,2017,22(2):157-164.
[6]孔祥崇,程文,郭存丽,等.乳腺癌腋窝淋巴结声像图联合免疫组织化学与淋巴结转移的相关性研究[J].中国癌症杂志,2016,26(2):155-160.
[7]吴佩琪,刘春玲,刘再毅,等.钼靶、CT与DCE-MRI评价乳腺癌淋巴结转移的价值[J].南方医科大学学报,2016,36(4):493-499.
[8]于庆龙,张莹,王宏健,等.17β-雌二醇对乳腺癌细胞迁移的影响及CANP-FN通路的介导作用[J].中国药理学通报,2017,33(10):1371-1376.
[9]朱蔼瑜,许宇媚,许晶,等.雄激素受体对雌激素受体阳性乳腺癌细胞增殖的抑制作用[J].医学研究生学报,2016,29(4):354-358.
[10]Secreto G,Muti P,Sant M,et al.Medical ovariectomy in menopausal breast cancer patients with high testosterone levels[J].Endocrine-related cancer,2017,24(11):21-29.
[11]刘静,季晨,史琳,等.抑制赖氨酰氧化酶可抑制胃癌细胞体外迁移和异质黏附[J].宁夏医科大学学报,2017,39(4):390-393.
[12]Wang TH,Hsia SM,Shieh TM.Lysyl oxidase and the tumor microenvironment[J].International Journal of Molecular Sciences,2017,18(1):62.
[13]Mahdavi M,Moreau V,Kheirollahi M.Identification of B and T cell epitope based peptide vaccine from IGF-1 receptor in breast cancer[J].Journal of Molecular Graphics&Modelling,2017,75(8):316-321.
[14]MatàR,Palladino C,Nicolosi ML,et al.IGF-I induces upregulation of DDR1 collagen receptor in breast cancer cells by suppressing MIR-199a-5p through the PI3K/AKT pathway[J].Oncotarget,2016,7(7):7683-7700.
[15]凌锐,周玲,周月鹏,等.Wnt1诱导分泌蛋白-1通过NF-κB通路促进食管鳞癌细胞的增殖和侵袭转移[J].江苏大学学报(医学版),2018,28(2):129-134.
[16]Mitrofanova I,Zavyalova M,Telegina N,et al.Tumor-associated macrophages in human breast cancer parenchyma negatively correlate with lymphatic metastasis after neoadjuvant chemotherapy[J].Immunobiology,2017,222(1):101-109.
[17]Shinozaki M,Hoon D,Giuliano A,et al.Distinct hypermethylation profile of primary breast cancer is associated with sentinel lymph node metastasis[J].Clinical Cancer Research,2016,11(6):2156-2162.
[18]张敏敏,韦薇,刘剑仑,等.乳腺癌组织LOX蛋白表达与腋窝淋巴结转移相关性分析[J].中华肿瘤防治杂志,2017,24(4):236-241.