A high-performance bio-tissue imaging method using air flow-assisted desorption electrospray ionization coupled with a high-resolution mass spectrometer

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英文篇名：A high-performance bio-tissue imaging method using air flow-assisted desorption electrospray ionization coupled with a high-resolution mass spectrometer 作者：Yiwei ; Lv ; Tiegang ; Li ; Chengan ; Guo ; Chenglong ; Sun ; Fei ; Tang ; Luojiao ; Huang ; Zhigang ; Luo ; Xin ; Li ; Ruiping ; Zhang ; Qingce ; Zang ; Jiuming ; He ; Zeper ; AbliZ 英文作者：Yiwei Lv;Tiegang Li;Chengan Guo;Chenglong Sun;Fei Tang;Luojiao Huang;Zhigang Luo;Xin Li;Ruiping Zhang;Qingce Zang;Jiuming He;Zeper AbliZ;State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College;State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University;Center for Imaging and Systems Biology, Minzu University of China; 英文关键词：Mass spectrometry imaging;;Air flow-assisted desorption electrospray;;ionization;;High-resolution mass spectrometry;;Endogenous metabolites;;Bio-tissue imaging 中文刊名：FXKB 英文刊名：中国化学快报(英文版) 机构：State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College;State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University;Center for Imaging and Systems Biology, Minzu University of China; 出版日期：2019-02-15 出版单位：Chinese Chemical Letters 年：2019 期：v.30 基金：supported by the National Instrumentation Program (No. 2016YFF0100304);; the National Natural Science Foundation of China(Nos. 21335007, 81773678);; the CAMS Innovation Fund for Medical Sciences(No. 2016-12 M-1-009);; PUMC Youth Fund and the Fundamental Research Funds for the Central Universities(No. 3332015177) 语种：英文; 页：FXKB201902045 页数：4 CN：02 ISSN：11-2710/O6 分类号：211-214

摘要

Mass spectrometry imaging (MSI) technology can simultaneously obtain the spatial distribution of thousands of chemical compounds and has unique advantages compared to other techniques that allow mapping the surface of bio-tissue. Here, we combined an air flow-assisted desorption electrospray ionization (AFADESI) MSI device with a high-resolution mass spectrometer to optimize the system parameters and achieve more accurate spatial distribution characteristics for compounds of interest while investigating bio-tissue sections. The platform set-up, required instrumentation, sample pretreatment, parameter optimization and bio-tissue characterization are described and discussed.Finally, the parameter conditions that can provide optimal ionic intensity and enhanced resolution were confirmed. The reasonable resolution and sensitivity improvements of AFADESI-MSI have been achieved through tandem a high-resolution mass spectrometer system, therefore, it would be a promising technique for the bio-tissue imaging analysis.
        Mass spectrometry imaging (MSI) technology can simultaneously obtain the spatial distribution of thousands of chemical compounds and has unique advantages compared to other techniques that allow mapping the surface of bio-tissue. Here, we combined an air flow-assisted desorption electrospray ionization (AFADESI) MSI device with a high-resolution mass spectrometer to optimize the system parameters and achieve more accurate spatial distribution characteristics for compounds of interest while investigating bio-tissue sections. The platform set-up, required instrumentation, sample pretreatment, parameter optimization and bio-tissue characterization are described and discussed.Finally, the parameter conditions that can provide optimal ionic intensity and enhanced resolution were confirmed. The reasonable resolution and sensitivity improvements of AFADESI-MSI have been achieved through tandem a high-resolution mass spectrometer system, therefore, it would be a promising technique for the bio-tissue imaging analysis.

引文

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