香蕉类受体蛋白激酶基因的克隆及杂交鉴定(英文)
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摘要
[目的]克隆鉴定香蕉类受体蛋白激酶基因。[方法]以香蕉果实cDNA噬菌体文库为材料,筛选出香蕉类受体蛋白激酶基因阳性噬菌体库,对该基因进行克隆和序列分析,并通过原位杂交方法对其进行鉴定。[结果]试验克隆到1个长度为1698bp的香蕉果实类受体蛋白激酶基因,编码563个氨基酸序列。经过Southern杂交证实该基因来自香蕉基因组,是1个多拷贝基因。[结论]该研究结果为进一步研究香蕉类受体蛋白激酶基因在香蕉果实中的功能奠定了基础。
[Objective] This study aimed to clone and identify the banana fruit receptor-like protein kinase gene.[Method] The cDNA phage libraries of banana fruit were adopted as the experimental materials to screen positive phage libraries of banana receptor-like protein kinase gene;cloning and sequence analysis of the gene were conducted,and the banana receptor-like protein kinase gene was identified by using in situ hybridization method.[Result] In this study,a 1 698 bp long banana receptor-like protein kinase gene was cloned from banana fruit,encoding 563 amino acids.Southern hybridization result confirmed that the banana receptor-like protein kinase gene was a multiple-copy gene from banana genome.[Conclusion] The study laid the foundation for further investigating the functions of banana receptor-like protein kinase gene in fruit.
[Objective] This study aimed to clone and identify the banana fruit receptor-like protein kinase gene.[Method] The cDNA phage libraries of banana fruit were adopted as the experimental materials to screen positive phage libraries of banana receptor-like protein kinase gene;cloning and sequence analysis of the gene were conducted,and the banana receptor-like protein kinase gene was identified by using in situ hybridization method.[Result] In this study,a 1 698 bp long banana receptor-like protein kinase gene was cloned from banana fruit,encoding 563 amino acids.Southern hybridization result confirmed that the banana receptor-like protein kinase gene was a multiple-copy gene from banana genome.[Conclusion] The study laid the foundation for further investigating the functions of banana receptor-like protein kinase gene in fruit.
引文
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[10]SU YM(苏咏梅),ZHU WJ(朱文进),ZHU YH(朱月华).Cloning and bioinformatics analysis of donkey growth hormone gene cDNA(驴生长激素基因cDNA的克隆及生物信息学分析)[J].Agricultural Science&Technology(农业科学与技术),2011,12(4):617-620.
[11]YAO QG(姚清国),LI XQ(李晓芹),ZHANG WN(张文娜),et al.Screening of extracellular binding proteins of rice Receptorlike kinase CR4by the yeast two-hybrid(通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白)[J].Agricultural Science&Technology(农业科学与技术),2010,11(12):77-81.
[12]BIAN MD(边鸣镝),DENG C(邓川),BAI ZY(白忠义).cDNA cloning of maize protein kinase gene ZmLRRPK1and its expression analysis(玉米类受体蛋白激酶基因ZmLRRPK1的cDNA克隆与表达分析)[J].China Biotechnology(中国生物工程杂志),2009,29(7):33-36.
[13]FANG XL(房孝良),LIU W(刘炜).Cloning and sequencing of a receptorlike protein kinase gene OsESG1in rice(水稻类受体蛋白激酶基因OsESG1的克隆与生物信息学分析)[J].Biotechnology Bulletin(生物技术通报),2012,3:166-172.
[14]YAO QG(姚清国),ZHOU EP(周二鹏),WANG J(王娟),et al.Screening of extracellular binding proteins of rice receptor-like Kinase CR4by the yeast two-hybrid(通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白)[J].Journal of Anhui Agricultural Sciences(安徽农业科学),2011,39(8):44274430.
[15]XU R,TANG XY,MIAO MM,et al.Construction of fusion expression vector ofα-galactosidase-EGFP in cucumber[J].Agricultural Science&Technology,2010,11(3):25-27.
[2]ZHANG CC(张承才),PENG L(彭玲).Function of protein kinase in plant growth and development(蛋白激酶在植物生长及发育中作用)[M]//XU ZH(许智宏),LIU CM(刘春明).Growth and Development of Plant Molecular Physiology(植物发育的分子生理).Beijing:Science Press(北京:科学出版社),1998:172-192.
[3]STONE JM,WALKER JC.Plant protein kinase families and signal transduction[J].Plant Physiol,1995,105:451-457.
[4]CHANG C,KWOK SF,BLEECKER AB et al.Arabidopsis ethylene-resporise gene ETR1:similarity of product to twocomponent regulators[J].Science,1993,162:539-543.
[5]LIU XL(刘小丽).The plant protein kinase and MAPK signal transduction system(植物蛋白质激酶和MAPK信号传递系统)[J].Journal of Hebei Normal University:Natural Science(河北师范大学学报:自然科学),2001,25(3):385388.
[6]ABO-EL-SAAD M,WU R.A rice membrane calcium-dependent protein kinase is induced by gibberelli[J].Plant Physiol,1995,108:787-793.
[7]GIRANTON JL,DUMAS C,COCK JM.The integral membrane S-locusreceptor kinase of Brassicahasserine/threonine kinase activity in a membranous environment and spontaneously form soligomers in planta[J].Proc Natl Acad Sci USA,2000,97(7):3759-3764.
[8]WEN PP(闻盼盼),GUO ZF(郭泽峰),XU LB(许林兵),et al.Application of mitochondria DNA hypervariable region in identification of genetic relationships among different varieties in Musa paradisiacal(线粒体基因高变区应用于香蕉种属亲缘关系的鉴定研究)[J].Agricultural Science&Technology(农业科学与技术),2010,11(8):91-93.
[9]JI LS(冀芦沙),WANG HX(王洪霞),GUO SJ(郭尚敬).Cloning and expression analysis of the high mobility B group genes in Arabidopsis thaliana(拟南芥高迁移率族蛋白B(HMGB)族基因的克隆及表达分析)[J].Agricultural Science&Technology(农业科学与技术),2010,11(8):160-163,172.
[10]SU YM(苏咏梅),ZHU WJ(朱文进),ZHU YH(朱月华).Cloning and bioinformatics analysis of donkey growth hormone gene cDNA(驴生长激素基因cDNA的克隆及生物信息学分析)[J].Agricultural Science&Technology(农业科学与技术),2011,12(4):617-620.
[11]YAO QG(姚清国),LI XQ(李晓芹),ZHANG WN(张文娜),et al.Screening of extracellular binding proteins of rice Receptorlike kinase CR4by the yeast two-hybrid(通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白)[J].Agricultural Science&Technology(农业科学与技术),2010,11(12):77-81.
[12]BIAN MD(边鸣镝),DENG C(邓川),BAI ZY(白忠义).cDNA cloning of maize protein kinase gene ZmLRRPK1and its expression analysis(玉米类受体蛋白激酶基因ZmLRRPK1的cDNA克隆与表达分析)[J].China Biotechnology(中国生物工程杂志),2009,29(7):33-36.
[13]FANG XL(房孝良),LIU W(刘炜).Cloning and sequencing of a receptorlike protein kinase gene OsESG1in rice(水稻类受体蛋白激酶基因OsESG1的克隆与生物信息学分析)[J].Biotechnology Bulletin(生物技术通报),2012,3:166-172.
[14]YAO QG(姚清国),ZHOU EP(周二鹏),WANG J(王娟),et al.Screening of extracellular binding proteins of rice receptor-like Kinase CR4by the yeast two-hybrid(通过酵母双杂交筛选水稻类受体激酶CR4的胞外结合蛋白)[J].Journal of Anhui Agricultural Sciences(安徽农业科学),2011,39(8):44274430.
[15]XU R,TANG XY,MIAO MM,et al.Construction of fusion expression vector ofα-galactosidase-EGFP in cucumber[J].Agricultural Science&Technology,2010,11(3):25-27.