一测多评法测定二母宁嗽丸中6种有效成分含量
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摘要
目的:建立一测多评法测定二母宁嗽丸中6种活性成分含量。方法:采用HPLC法,流动相为甲醇(A)-0.1%磷酸水溶液(B)梯度洗脱,流速为1.0 ml·min~(-1),检测波长237 nm,柱温30℃,进样量10μl;以黄芩苷为内标物,建立一测多评法同时测定橙皮苷、甘草苷、甘草酸铵、甘草次酸和五味子醇甲5种成分的相对较正因子(RCF)并计算其含量,并与外标法分析比较以上6种成分的含量差异。结果:一测多评法测得的黄芩苷、橙皮苷、甘草苷、甘草酸铵、甘草次酸和五味子醇甲6种成分含量计算值与外标法测定值的差异无统计学意义(P>0.05)。结论:一测多评法可用于二母宁嗽丸中6种有效成分的含量测定,方法简单、有效、结果准确。
Objective: To develop a method of quantitative analysis of multi-components by single marker for six effective constituents in Ermu Ningsou pills. Methods: Using an HPLC method,the mobile phase consisted of methanol-0. 1% phosphoric acid solution with gradient elution. The flow rate was 1. 0 ml·min~(-1),the detection wavelength was set at 237 nm,the column temperature was 30℃ and the injection volume was 10 μl. Using baicalin as the internal standard,the relative correction factor among hesperidin,liquiriti,glycyrram,glycyrrhetinic acid and schisandrin were detected by QAMS and their contents were calculated,and then compare with those of an external standard method. Results: There were no significant differences between the calculated values in QAMS and the estimated values in the external standard method( P > 0. 05). Conclusion: The QAMS method can be used for the determination of six effective constituents in Ermu Ningsou pills,which is simple,effective and accurate.
Objective: To develop a method of quantitative analysis of multi-components by single marker for six effective constituents in Ermu Ningsou pills. Methods: Using an HPLC method,the mobile phase consisted of methanol-0. 1% phosphoric acid solution with gradient elution. The flow rate was 1. 0 ml·min~(-1),the detection wavelength was set at 237 nm,the column temperature was 30℃ and the injection volume was 10 μl. Using baicalin as the internal standard,the relative correction factor among hesperidin,liquiriti,glycyrram,glycyrrhetinic acid and schisandrin were detected by QAMS and their contents were calculated,and then compare with those of an external standard method. Results: There were no significant differences between the calculated values in QAMS and the estimated values in the external standard method( P > 0. 05). Conclusion: The QAMS method can be used for the determination of six effective constituents in Ermu Ningsou pills,which is simple,effective and accurate.
引文
1中国药典[S].2015年版.一部.435-436
2 刘莉,周世玉,丁叶,等.龙胆泻肝胶囊中甘草苷、甘草酸铵的含量测定[J].内江科技,2017,38(7):29-30
3 于霞,吴娟,姜雪,等.HPLC法同时测定消乳散中甘草苷、橙皮苷和甘草酸铵的含量[J].沈阳药科大学学报,2017,34(5):369-372
4 蔡雁,于海英.HPLC法同时测定复方天麻颗粒中天麻素和五味子醇甲的含量[J].中国药师,2018,21(8):1480-1482
5 金慧臻,黄艳,毛晨梅,等.金蝉口服液中黄芩苷的HPLC法含量测定[J].抗感染药学,2018,15(6):937-939
6 李岩岩,周妍.HPLC法测定牛黄芩连丸中黄芩苷含量[J].世界最新医学信息文摘,2018,18(61):12-14
7 高厚明,胡兆流,郑平,等.HPLC-MS测定小儿安神补脑颗粒中8个成分的含量[J].中国实验方剂学杂志,2018,24(19):73-78
8 关皎,王强,孙宇慧,等.UFLC法同时测定甘草及其炮制品中2种活性成分的含量[J].吉林医药学院学报,2018,39(4):241-243
9 张方,郜红利.一测多评法同时测定舒血宁注射液中6种黄酮类成分[J].中国药师,2015,18(10):1652-1656
10 赖增发,姜建伟.一测多评法测定香砂六君丸中木香内酯类成分[J].中国药师,2013,16(11):1659-1662
11 伍静玲,仲海洁,胡军华,等.一测多评法同时测定苏子油胶丸中5 种不饱和脂肪酸的含量[J].中国药房,2016,27(12):1655-1658
12 孙玲,樊晓兰,郭绮,等.一测多评法同时测定山银花药材中5种皂苷类成分的含量[J].中国药房,2017,28(18):2546-2549
13 张秀丽,吴楠.HPLC法测定二母宁嗽丸中黄芩苷的含量[J].中国医药导报,2009,6(11):45-46
14 彭柳,周俊,范开静.间接火焰原子吸收法测定二母宁嗽丸中芒果苷的含量[J].中国实验方剂学杂志,2015,21(1):95-98
15 常志惠,胡军华,万亚菲,等.一测多评法同时测定保肾乙丸中5种蒽醌类成分的含量[J].中国药房,2016,27(36):5156-5159
2 刘莉,周世玉,丁叶,等.龙胆泻肝胶囊中甘草苷、甘草酸铵的含量测定[J].内江科技,2017,38(7):29-30
3 于霞,吴娟,姜雪,等.HPLC法同时测定消乳散中甘草苷、橙皮苷和甘草酸铵的含量[J].沈阳药科大学学报,2017,34(5):369-372
4 蔡雁,于海英.HPLC法同时测定复方天麻颗粒中天麻素和五味子醇甲的含量[J].中国药师,2018,21(8):1480-1482
5 金慧臻,黄艳,毛晨梅,等.金蝉口服液中黄芩苷的HPLC法含量测定[J].抗感染药学,2018,15(6):937-939
6 李岩岩,周妍.HPLC法测定牛黄芩连丸中黄芩苷含量[J].世界最新医学信息文摘,2018,18(61):12-14
7 高厚明,胡兆流,郑平,等.HPLC-MS测定小儿安神补脑颗粒中8个成分的含量[J].中国实验方剂学杂志,2018,24(19):73-78
8 关皎,王强,孙宇慧,等.UFLC法同时测定甘草及其炮制品中2种活性成分的含量[J].吉林医药学院学报,2018,39(4):241-243
9 张方,郜红利.一测多评法同时测定舒血宁注射液中6种黄酮类成分[J].中国药师,2015,18(10):1652-1656
10 赖增发,姜建伟.一测多评法测定香砂六君丸中木香内酯类成分[J].中国药师,2013,16(11):1659-1662
11 伍静玲,仲海洁,胡军华,等.一测多评法同时测定苏子油胶丸中5 种不饱和脂肪酸的含量[J].中国药房,2016,27(12):1655-1658
12 孙玲,樊晓兰,郭绮,等.一测多评法同时测定山银花药材中5种皂苷类成分的含量[J].中国药房,2017,28(18):2546-2549
13 张秀丽,吴楠.HPLC法测定二母宁嗽丸中黄芩苷的含量[J].中国医药导报,2009,6(11):45-46
14 彭柳,周俊,范开静.间接火焰原子吸收法测定二母宁嗽丸中芒果苷的含量[J].中国实验方剂学杂志,2015,21(1):95-98
15 常志惠,胡军华,万亚菲,等.一测多评法同时测定保肾乙丸中5种蒽醌类成分的含量[J].中国药房,2016,27(36):5156-5159