中风1号对急性脑出血大鼠磷脂酰肌醇3-激酶/蛋白激酶B信号通路影响
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摘要
目的:观察中风1号汤剂对急性脑出血大鼠磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/AKT)信号通路的影响。方法:将50只雄性Sprague-Dawley大鼠随机分为5组(中风1号组、甘露醇组、模型组、假手术组、空白组),每组10只,并给予造模,应用荧光定量RT-PCR技术检测各组脑出血大鼠脑组织中磷脂酰肌醇3-激酶和蛋白激酶B的表达水平,运用2-△△CT法分析结果。结果:中风1号组PI3K和AKT相关表达水平相对于模型组显著升高(P<0.05)。结论:中风1号汤剂在急性脑出血时对组织起到的脑保护作用与其激活了PI3K/AKT信号通路有关。
Objective:To observe the effect of decoction of Zhongfeng No.1 on phosphatidylinositol 3-kinase/protein kinase B(PI3 K/AKT)signaling pathway in rats with acute cerebral hemorrhage. Methods:Fifty male Sprague-Dawley rats were randomly divided into 5 groups(Zhongfeng No.1 group,mannitol group,model group,sham operation group,and blank group)with 10 rats in each group. RT-PCR was used to detect the expression of phosphoinositide 3-kinase and protein kinase B in the brains of rats with intracerebral hemorrhage. The results were analyzed by 2-△△CT. Results:The expression levels of PI3 K and AKT in Zhongfeng No.1 group were significantly increase from those in the model group(P<0.05). Conclusion:The brain protective effect of Zhongfeng No.1 Decoction on acute cerebral hemorrhage is related to its activation of PI3 K/AKT signaling pathway.
Objective:To observe the effect of decoction of Zhongfeng No.1 on phosphatidylinositol 3-kinase/protein kinase B(PI3 K/AKT)signaling pathway in rats with acute cerebral hemorrhage. Methods:Fifty male Sprague-Dawley rats were randomly divided into 5 groups(Zhongfeng No.1 group,mannitol group,model group,sham operation group,and blank group)with 10 rats in each group. RT-PCR was used to detect the expression of phosphoinositide 3-kinase and protein kinase B in the brains of rats with intracerebral hemorrhage. The results were analyzed by 2-△△CT. Results:The expression levels of PI3 K and AKT in Zhongfeng No.1 group were significantly increase from those in the model group(P<0.05). Conclusion:The brain protective effect of Zhongfeng No.1 Decoction on acute cerebral hemorrhage is related to its activation of PI3 K/AKT signaling pathway.
引文
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[2]Li Z,He Q,Zhai X,et al. Foxo1-mediated InflammatoryResponse After Cerebral Hemorrhage in Rats[J]. NeuroscienceLetters,2016,629:131-136.
[3]周晓东.中医针灸配合康复理疗治疗脑出血的临床效果[J].中医临床研究,2016,8(14):103-104.
[4]梁群,朱永志,杨滢,等.中风1号汤剂对急性脑出血大鼠NSE蛋白水平影响及改善神经功能缺损的实验研究[J].中医药信息,2011,28(6):120-122.
[5]LiuD,HouP,LiuZ,etal.Geneticalterationsinthephosphoinositide3-kinase/Aktsignalingpathwayconfersensitivity of thyroid cancer cells to therapeutic targe-ting of Aktand mammalian target of rapamycin[J]. Cancer Res,2009,69(18):7311-7319.
[6]郁迪. PI3K/Akt和MAPK信号通路在缺血性脑损伤中的保护作用[J].医学综述,2015,21(2):210-213.
[7]张亚红,刘重霄,周任,等.大鼠脑出血模型中血脑屏障开放规律及应用脑血疏口服液治疗脑水肿的实验研究[J].中西医结合心脑血管病杂志,2016,14(14):1613-1615.
[8]刘志明,黄山,常柄权,等.藏荆芥提取物对脑出血大鼠AQP-4及NGF表达的影响[J].中国实验方剂学杂志, 2018(9):150-154.
[9]JD Neves,D Aristimunha,AF Vizuete,et al. Glial-associatedchanges in the cerebral cortex after collagenase-inducedintracerebral hemorrhage in the rat striatum[J]. Brain ResearchBulletin, 2017,134:55-62.
[10]YW Tang,S Meng,H Li,et al. PCR Enhances Acid-FastBacillus Stain-Based Rapid Detection of Mycobacteriumtuberculosis[J]. Journal of Clinical Microbiology, 2016,42(4):1849-1850.
[11]J Quick,ND Grubaugh,ST Pullan,et al. Multiplex PCRmethod for MinION and Illumina sequencing of Zika andother virus genomes directly from clinical samples[J]. NatureProtocols, 2017,12(6):1261.
[12]陈敬,刘钰,汤重发,等.小鼠胃组织中幽门螺杆菌SYBRGreenⅡ实时定量PCR检测方法的建立及验证[J].中国生物制品学杂志,2016,29(12):1308-1315.
[13]Pellegrini C,Falleni M,Marchetti A,et al. HER 2/Neualterations in non-small cell lung cancer:a comprehensiveevaluation by real time reverse transcription-PCR,fluorescencein situ Hybridization,and immunohisto-chemistry[J]. ClinCancer Res,2003,9(10):3645.
[14]Wong SM,Jansen JFA,Zhang CE,et al. Measuring subtleleakage of the blood-brain barrier in cerebrovascular diseasewith DCE-MRl:Test-retest reproducibility and its influencingfactors[J]. J Magn Reson Imaging,2017,46(1):159-166.
[15]MY Xu,SQ Liu,CL Deng,et al. Detection of Zika virus bySYBR green one-step real-time RT-PCR[J]. Journal ofVirological Methods,2016,236:93-97.
[16]R Zhang,H Hardin,W Huang,et al. MALAT1 Long Non-coding RNA Expression in Thyroid Tissues:Analysis by In SituHybridization and Real-Time PCR[J]. Endocrine Pathology,2017,28(1):7-12.
[17]吴晓光,李蒙蒙,仇志富,等.补阳还五汤对脑出血大鼠PI3K/AKT信号通路的影响及其神经保护作用的机制[J].国际神经病学神经外科学杂志,2016,43(2):119-123.
[18]向勇,朱建萍,刘丹荣.rhEPO通过调节PI3K/AKT信号通路改善大鼠脑出血后神经元损伤[J].中南医学科学杂志,2017,45(5):454-458.
[19]J Li,Z Song,Y Wang,Y Yin,et al. Overexpression of SphK1enhances cell proliferation and invasion in triple-negative breastcancer via the PI3K/AKT signaling pathway[J]. Tumor Biology,2016,37(8):1-7.
[20]仇志富,吴晓光,孟杰,等.补阳还五汤提取物灌胃对脑出血大鼠脑组织中PI3K、AKT、caspase-3表达的影响[J].山东医药,2016,56(9):29-31.
[21]刘志恒,曹圆圆.中西医结合疗法在脑出血急性期治疗中的应用[J].中医临床研究,2017,9(8):81-82.
[22]李蕊,王昀,赵海滨,等.中医药治疗急性脑出血的临床研究进展[J].世界中西医结合杂志,2018(3):441-444.
[23]薛群星,赵炳海,边群,等.羚角钩藤汤化裁联合注射用鼠神经生长因子对急性脑出血患者脑血流动力学及炎症因子的影响[J].现代中西医结合杂志,2017,26(4):393-395.
[24]周艳霞,韩漫夫,黄楚明,等.腔隙性脑梗死与小量脑出血患者脑动静脉血流动力学特征分析[J].疑难病杂志,2016,15(2):111-115.
[25]Rumalla K,Smith KA,Arnold PM,et al. SubarachnoidHemorrhage and Readmissions:National Rates,Causes,RiskFactors,and Outcomes in 16,001 Hospitalized Patients[J].World Neurosurg,2018,110:e100-e111.
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[27]马玉兰,马青海,马骁.补阳还五汤合三七粉治疗缺血性中风恢复期气虚血瘀证的疗效观察[J].中药药理与临床,2016(5):93-96.