Mn-ZnS量子点室温磷光检测尿液中硫酸庆大霉素
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摘要
目的:基于硫酸庆大霉素对Mn掺杂ZnS量子点的磷光增敏作用,建立一种简便且灵敏测定硫酸庆大霉素浓度的新方法。方法:在水相中合成巯基丙酸(MPA)包裹的Mn掺杂ZnS量子点,实验中考察了缓冲溶液pH和类型、反应时间及量子点浓度等因素对室温磷光检测的影响。利用荧光分光光度计通过测定Mn掺杂ZnS量子点磷光强度的变化,实现对尿液中硫酸庆大霉素以及硫酸庆大霉素注射液浓度的测定。结果:成功合成了浓度为2×10~(-2)mg/L的Mn掺杂ZnS量子点。在pH=7.4的三羟甲基氨基甲烷-盐酸缓冲体系中,静置反应时间为30min条件下,硫酸庆大霉素浓度与Mn掺杂ZnS量子点磷光强度线性关系良好且相关系数R~2=0.9985。结论:该方法灵敏、快捷,可以用于测定尿液中硫酸庆大霉素的浓度。
Objective: To establish a simple and sensitive method for the determination of gentamicin sulfate based on the photosensitization of Mn-Zn S quantum dots. Methods: propionic acid(MPA) modified Mn-Zn S quantum dots were synthesized in aqueous phase. The effects of pH,type of buffer solution, reaction time and concentration of quantum dots on the room-temperature phosphorescent detection were investigated.The concentrations of gentamicin sulfate injection and gentamicin sulfate in urine were determined by measuring the phosphorescence intensity of Mn-Zn S quantum dots with a fluorescence spectrophotometer. Results: The Mn-Zn S quantum dots were successfully synthesized. The linear relationship between gentamicin sulfate and phosphorescence intensity of the Mn-Zn S quantum dots(2 ×10~(-2) mg/L) was obtained with a correlation of 0.998 5 after 30 min incubation in Tris-HCl buffer(pH =7.4) at room temperature. Conclusion:The proposed method is sensitive and simple and can be used for the determination of gentamicin sulfate in urine.
Objective: To establish a simple and sensitive method for the determination of gentamicin sulfate based on the photosensitization of Mn-Zn S quantum dots. Methods: propionic acid(MPA) modified Mn-Zn S quantum dots were synthesized in aqueous phase. The effects of pH,type of buffer solution, reaction time and concentration of quantum dots on the room-temperature phosphorescent detection were investigated.The concentrations of gentamicin sulfate injection and gentamicin sulfate in urine were determined by measuring the phosphorescence intensity of Mn-Zn S quantum dots with a fluorescence spectrophotometer. Results: The Mn-Zn S quantum dots were successfully synthesized. The linear relationship between gentamicin sulfate and phosphorescence intensity of the Mn-Zn S quantum dots(2 ×10~(-2) mg/L) was obtained with a correlation of 0.998 5 after 30 min incubation in Tris-HCl buffer(pH =7.4) at room temperature. Conclusion:The proposed method is sensitive and simple and can be used for the determination of gentamicin sulfate in urine.
引文
[1]朱立勤,娄建石,焦建杰,等.庆大霉素不同给药方案的药代动力学和肾功能损害的实验研究[J].中国药理学通报,2006,22(8):1009
[2]Garneau-Tsodikova S,Labby K J.Mechanisms of resistance to aminoglycoside antibiotics:Overview and perspectives[J].Medchemcomm,2016,7(1):11
[3]Li J,Woo C W.Finding ways to solve or preventaminoglycoside-inducedototoxicity[J].Ann Transl Med,2016,4(24):533
[4]Gerlach A T,Stawicki S P,Cook CH,et al.Risk factors for aminoglycoside-associated nephrotoxicity in surgical intensive care unit patients[J].Int J Crit Illn Inj Sci,2011,1(1):17
[5]Touw D J,Westerman E M,Sprij A J.Therapeutic drug monitoring of aminoglycosides in neonates[J].Clin Pharmacokinet,2009,48(2):71
[6]曾萍,杨镒宇,陶建平,等.危重病患儿口服庆大霉素血药质量浓度监测的意义[J].实用儿科临床杂志,2006,21(6):339
[7]彭小林,范亚新,张亮,等.氨基糖苷类抗生素治疗药物浓度监测的研究进展[J].中国感染与化疗杂志,2017,17(1):104
[8]Mahmoudi L,Mohammadpour A H,Ahmadi A,et al.Influence of sepsis on higher daily dose of amikacin pharmacokinetics in critically ill patients[J].Eur Rev Med Pharmacol Sci,2013,17(3):285
[9]Rodriquez M,Cretoso D S,Euterpio M A,et al.Fast determination of underivatized gentamicin C components and impurities by LC-MS using a porous graphitic carbon stationary phase[J].Anal Bioanal Chem,2015,407(25):7691
[10]李玮,胡昌勤,王明娟.高效液相色谱法测定庆大霉素C组分的不同检测方式测定结果的比较[J].色谱,2007,25(4):557
[11]杨利红,常艳,姚尚辰,等.HPLC法同时测定庆大霉素的组分纯度和效价活性[J].药学学报,2012,12:105
[12]潘进,邱凌峰,方圣琼,等.衍生化紫外分光光度法测定硫酸庆大霉素含量[J].环境工程,2015,33:295
[13]Tan L,Li Y,Tang Y,et al.Room temperature phosphorescence sensor for Hg2+based on Mn-doped Zn S quantum dots[J].J Nanosci Nanotechnol,2012,12(10):7788
[14]Zhao M X,Zeng E Z.Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging[J].Nanoscale Res Lett,2015,10(1):171
[15]Wu P,He Y,Wang H F,et al.Conjugation of glucose oxidase onto Mn-doped Zn S quantum dots for phosphorescent sensing of glucose in biological fluids[J].Anal Chem,2010,82(4):1427
[16]Zhang Z,Miao Y,Zhang Q,et al.Facile and sensitive detection of protamine by enhanced room-temperature phosphorescence of Mndoped Zn S quantum dots[J].Anal Biochem,2015,478:90
[2]Garneau-Tsodikova S,Labby K J.Mechanisms of resistance to aminoglycoside antibiotics:Overview and perspectives[J].Medchemcomm,2016,7(1):11
[3]Li J,Woo C W.Finding ways to solve or preventaminoglycoside-inducedototoxicity[J].Ann Transl Med,2016,4(24):533
[4]Gerlach A T,Stawicki S P,Cook CH,et al.Risk factors for aminoglycoside-associated nephrotoxicity in surgical intensive care unit patients[J].Int J Crit Illn Inj Sci,2011,1(1):17
[5]Touw D J,Westerman E M,Sprij A J.Therapeutic drug monitoring of aminoglycosides in neonates[J].Clin Pharmacokinet,2009,48(2):71
[6]曾萍,杨镒宇,陶建平,等.危重病患儿口服庆大霉素血药质量浓度监测的意义[J].实用儿科临床杂志,2006,21(6):339
[7]彭小林,范亚新,张亮,等.氨基糖苷类抗生素治疗药物浓度监测的研究进展[J].中国感染与化疗杂志,2017,17(1):104
[8]Mahmoudi L,Mohammadpour A H,Ahmadi A,et al.Influence of sepsis on higher daily dose of amikacin pharmacokinetics in critically ill patients[J].Eur Rev Med Pharmacol Sci,2013,17(3):285
[9]Rodriquez M,Cretoso D S,Euterpio M A,et al.Fast determination of underivatized gentamicin C components and impurities by LC-MS using a porous graphitic carbon stationary phase[J].Anal Bioanal Chem,2015,407(25):7691
[10]李玮,胡昌勤,王明娟.高效液相色谱法测定庆大霉素C组分的不同检测方式测定结果的比较[J].色谱,2007,25(4):557
[11]杨利红,常艳,姚尚辰,等.HPLC法同时测定庆大霉素的组分纯度和效价活性[J].药学学报,2012,12:105
[12]潘进,邱凌峰,方圣琼,等.衍生化紫外分光光度法测定硫酸庆大霉素含量[J].环境工程,2015,33:295
[13]Tan L,Li Y,Tang Y,et al.Room temperature phosphorescence sensor for Hg2+based on Mn-doped Zn S quantum dots[J].J Nanosci Nanotechnol,2012,12(10):7788
[14]Zhao M X,Zeng E Z.Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging[J].Nanoscale Res Lett,2015,10(1):171
[15]Wu P,He Y,Wang H F,et al.Conjugation of glucose oxidase onto Mn-doped Zn S quantum dots for phosphorescent sensing of glucose in biological fluids[J].Anal Chem,2010,82(4):1427
[16]Zhang Z,Miao Y,Zhang Q,et al.Facile and sensitive detection of protamine by enhanced room-temperature phosphorescence of Mndoped Zn S quantum dots[J].Anal Biochem,2015,478:90