Mn掺杂ZnS量子点荧光猝灭法测定对乙酰氨基酚
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摘要
利用水热合成法合成了L-谷胱甘肽(GSH)修饰的Mn掺杂ZnS量子点(QDs),基于对乙酰氨基酚对该量子点的荧光猝灭作用,建立了一种快速高效检测对乙酰氨基酚的方法.在最优实验条件下,量子点荧光强度的变化与对乙酰氨基酚浓度在0.25~10μmol·L-1范围内呈良好的线性关系(R=0.999 5),检出限为4.1×10-8mol·L-1,相对标准偏差为0.35%.该方法成功地应用于人尿液中对乙酰氨基酚的测定,样品加标回收率为95%~100%.
L-Glutathione(GSH) modified Mn-doped ZnS quantum dots(QDs) were prepared using hydrothermal synthesis. Based on the fluorescence quenching of the QDs by acetaminophen,a fast and efficient detection method for acetaminophen was developed. Under optimized experiment conditions,the change of fluorescence intensity had a good linear relationship with the concentration of acetaminophen(0.25-10 μmol·L-1),the detection limit and relative standard deviation were 4.1×10-8 mol·L-1 and 0.35%,respectively. The method has been successfully applied for the detection of acetaminophen in the human urine with the recoveries of 95%-100%.
L-Glutathione(GSH) modified Mn-doped ZnS quantum dots(QDs) were prepared using hydrothermal synthesis. Based on the fluorescence quenching of the QDs by acetaminophen,a fast and efficient detection method for acetaminophen was developed. Under optimized experiment conditions,the change of fluorescence intensity had a good linear relationship with the concentration of acetaminophen(0.25-10 μmol·L-1),the detection limit and relative standard deviation were 4.1×10-8 mol·L-1 and 0.35%,respectively. The method has been successfully applied for the detection of acetaminophen in the human urine with the recoveries of 95%-100%.
引文
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[3]WILLIAM A,TOBY L,GEORGE C,et al.2004 Annual report of the american association of poison control centers toxic exposure surveillance system[J].The American Journal of Emergency Medicine,2005,23(5):589-666.
[4]张金磊,黄广君,兰翠玲,等.新铜试剂分光光度法测定片剂中的对乙酰氨基酚含量及其反应机理的研究[J].化学研究与应用,2015,27(10):1518-1522.ZHANG J L,HUANG G J,LAN C L,et al.Determination of acetaminophen content in tablets by neocuproine spectrophotometry and the reaction mechanism research[J].Chemical Research and Application,2015,27(10):1518-1522.
[5]TUREK A K,SIKORA M,RYBICKI M,et al.Frontally eluted components procedure with thin layer chromatography as a mode of sample preparation for high performance liquid chromatography quantitation of acetaminophen in biological matrix[J].Journal of Chromatography A,2016,1436:19-27.
[6]沙翠翠,范跃娟,周学莉,等.碳纤维微电极伏安法测定对乙酰氨基酚[J].化学研究,2015,26(4):389-393.SHA C C,FAN Y J,ZHOU X L,et al.Voltammetric determination of acetaminophen with carbon fiber microelectrode[J].Chemical Research,2015,26(4):389-393.
[7]关瑾,牛秋玲,何传昌,等.毛细管电泳法测定对乙酰氨基酚的含量[J].华西药学杂志,2013,28(6):615-616.GUAN J,NIU Q L,HE C C,et al.Determination of paracetamol by high performance capillary electrophoresis[J].West China Journal of Pharmaceutical Sciences,2013,28(6):615-616.
[8]刘益庆,李一,戴铭骏.HPLC-ELSD法测定小儿氨酚烷胺颗粒中对乙酰氨基酚和盐酸金刚烷胺的含量[J].西北药学杂志,2017,32(2):158-160.LIU Y Q,LI Y,DAI M J.Determination of the content of paracetamol and amantadine hydrochloride in pediatric paracetamol and amantadine hydrochloride granules by HPLC-ELSD[J].Northwest Pharmaceutical Journal,2017,32(2):158-160.
[9]WANG Z Y,FAN Z F.Cu2+modulated nitrogen-doped grapheme quantum dots as a turn-off/on fluorescence sensor for the selective detection of histidine in biological fluid[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2018,189:195-201.
[10]BHATTACHARYYA D,SARSWAT P K,FREE M L.Quantum dots and carbon dots based fluorescent sensors for TB biomarkers detection[J].Vacuum,2017,146:606-613.
[11]TAN X P,LIU S P,SHEN Y Z,et al.Quantum dots(QDs)based fluorescence probe for the sensitive determination of kaempferol[J].Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy,2014,133:66-72.
[12]CHULLASAT K,NURERK P,KANATHARANA P,et al.A facile optosensing protocol based on molecularly imprinted polymer coated on Cd Te quantum dots for highly sensitive and selective amoxicillin detection[J].Sensors and Actuators B:Chemical,2018,254:255-263.
[13]PACHECO M E,CASTELLS C B,BRUZZONE L.Mndoped Zn S phosphorescent quantum dots:Coumarins optical sensors[J].Sensors and Actuators B:Chemical,2017,238:660-666.
[14]VAISHANAV S K,KORRAM J,NAGWANSHI R,et al.Mn2+doped-Cd Te/Zn S modified fluorescence nanosensor for detection of glucose[J].Sensors and Actuators B:Chemical,2017,245:196-204.
[15]LI Y,MIAO Y M,YANG M Q,et al.DNA detection based on Mn-doped Zn S quantum dots/methylene blue nanohybrids[J].Chinese Chemical Letters,2016,27(5):773-778.
[16]CHEN Z Q,LIAN C,ZHOU D,et al.Greatly enhanced and controlled manganese photoluminescence in watersoluble Zn Cd S:Mn/Zn S core/shell quantum dots[J].Chemical Physics Letters,2010,488:73-76.