基于Mn掺杂ZnS量子点的室温磷光检测吡柔比星
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摘要
以3-巯基丙酸(MPA)为稳定剂采用水相合成法合成Mn掺杂Zn S量子点,基于MPA包裹的Mn掺杂Zn S量子点的室温磷光性质,建立了一种高效、灵敏的检测蒽醌类抗癌药物吡柔比星(Pirarubicin,THP)的新方法。在p H=7.4的磷酸盐缓冲溶液中,THP通过光诱导电子转移可以猝灭Mn掺杂Zn S QDs在波长590 nm处的磷光,且在一定范围内THP的浓度与磷光猝灭强度呈正比例关系,线性范围为0.3~16.2μg/m L,相关系数为0.992,方法检出限为0.072μg/m L。
Using 3-mercaptopropionic acid( MPA) as the stabilizer,MPA-capped Mn-doped Zn S QDs were synthesized via water phase method. Based on the room temperature phosphorescence properties of quantum dots,a highperformance and high-sensitivity method was established for detection of anthraquinone anticancer drug pirarubicin( THP). In the phosphate buffer of p H = 7. 4,the room temperature phosphorescence( RTP) at 590 nm of Mn-doped quantum dots were quenched through photoinduced electron transfer principle,the concentration of THP had a good linear relationship with the phosphorescence quenching intensity,linear scope of 0. 3 ~ 16. 2 μg/m L,and the detection limit and standard addition recovery of 0. 072 μg/m L( correlation coefficient R = 0. 992).
Using 3-mercaptopropionic acid( MPA) as the stabilizer,MPA-capped Mn-doped Zn S QDs were synthesized via water phase method. Based on the room temperature phosphorescence properties of quantum dots,a highperformance and high-sensitivity method was established for detection of anthraquinone anticancer drug pirarubicin( THP). In the phosphate buffer of p H = 7. 4,the room temperature phosphorescence( RTP) at 590 nm of Mn-doped quantum dots were quenched through photoinduced electron transfer principle,the concentration of THP had a good linear relationship with the phosphorescence quenching intensity,linear scope of 0. 3 ~ 16. 2 μg/m L,and the detection limit and standard addition recovery of 0. 072 μg/m L( correlation coefficient R = 0. 992).
引文
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[2]Patra S,Satpati B,Pradhan S K.Quickest single-step mechanosynthesis of Cd S quantum dots and their microstructure characterization[J].Journal of nanoscience and nanotechnology,2011,11(6):4771-4780.
[3]Yoshimura A,Mizukami S,Hori Y,et al.Cell‐Surface Protein Labeling with Luminescent Nanoparticles through Biotinylation by Using Mutantβ‐Lactamase‐Tag Technology[J].Chem Bio Chem,2011,12(7):1031-1034.
[4]Geszke M,Murias M,Balan L,et al.Folic acid-conjugated core/shell Zn S:Mn/Zn S quantum dots as targeted probes for two photon fluorescence imaging of cancer cells[J].Acta biomaterialia,2011,7(3):1327-1338.
[5]Briscoe J,Dunn S.Extremely thin absorber solar cells based on nanostructured semiconductors[J].Materials Science and Technology,2011,27(12):1741-1756.
[6]Su S,Wu W,Gao J,et al.Nanomaterials-based sensors for applications in environmental monitoring[J].Journal of Materials Chemistry,2012,22(35):18101-18110.
[7]Ertas N,Kara H E S.l-Cysteine capped Mn-doped Zn S quantum dots as a room temperature phosphorescence sensor for in-vitro binding assay of idarubicin and DNA[J].Biosensors and Bioelectronics,2015,70:345-350.
[8]He Y,Wang H F,Yan X P.Exploring Mn-doped Zn S quantum dots for the room-temperature phosphorescence detection of enoxacin in biological fluids[J].Analytical chemistry,2008,80(10):3832-3837.
[9]Lu Z,Zhang Y,Liu H,et al.Transport of a cancer chemopreventive polyphenol,resveratrol:interaction with serum albumin and hemoglobin[J].Journal of fluorescence,2007,17(5):580-587.
[10]Costa-Fernández J M,Pereiro R,Sanz-Medel A.The use of luminescent quantum dots for optical sensing[J].Tr AC Trends in Analytical Chemistry,2006,25(3):207-218.
[11]Sánchez-Barragán I,Costa-Fernández J M,Sanz-Medel A,et al.Room-temperature phosphorescence(RTP)for optical sensing[J].Tr AC Trends in Analytical Chemistry,2006,25(10):958-967.
[12]UMEZAWA H,TAKAHASHI Y,TAKEUCHI T,et al.The absolute structures of THP-adriamycins[J].The Journal of antibiotics,1984,37(9):1094-1097.
[13]He Y,Wang H F,Yan X P.Exploring Mn-doped Zn S quantum dots for the room-temperature phosphorescence detection of enoxacin in biological fluids[J].Analytical chemistry,2008,80(10):3832-3837.
[14]Costa-Fernández J M,Pereiro R,Sanz-Medel A.The use of luminescent quantum dots for optical sensing[J].Tr AC Trends in Analytical Chemistry,2006,25(3):207-218.
[15]Wu H,Wu J,Saez C,et al.Luminescence response of an osmium(Ⅱ)complex to macromolecular polyanions for the detection of heparin and chondroitin sulfate in biomedical preparations[J].Analytica chimica acta,2013,804:221-227.
[16]Tsuruo T,Iida H,Tsukagoshi S,et al.4'-OTetrahydropyranyladriamycin as a potential new antitumor agent[J].Cancer research,1982,42(4):1462-1467.
[17]HISAMATSU T,Suzu KI K,SAKAKIBARA S,et al.Antitumor spectrum of a new anthracycline,(2"R)-4'-O-tetrahydropyranyladriamycin,and effect on the cellular immune response in mice[J].Japanese Journal of Cancer Research GANN,1985,76(10):1008-1020.
[18]Majima H,Ohta K.Clinical studies of(2''R)-4'-Otetrahydropyranyl adriamycin(THP)[J].Biomedicine&pharmacotherapy=Biomedecine&pharmacotherapie,1987,41(5):237-243.
[19]孙宏斌,刘军,苏江浩,等.吡柔比星膀胱灌注预防浅表性膀胱癌术后复发[J].临床泌尿外科杂志,2002,17(6):284-285.
[20]Miller A A,Schmidt C G.Clinical pharmacology and toxicity of 4'-O-tetrahydropyranyladriamycin[J].Cancer research,1987,47(5):1461-1465.
[21]Dantchev D,Paintrand M,Hayat M,et al.Low heart and skin toxicity of a tetrahydropyranyl derivative of adriamycin(THP-ADM)as observed by electron and light microscopy[J].The Journal of antibiotics,1979,32(10):1085-1086.
[22]张琰,程建峰.高效液相色谱法检测吡柔比星的血药浓度[J].中国抗生素杂志,2000,25(5):356-358.
[23]Zhuang J,Zhang X,Wang G,et al.Synthesis of water-soluble Zn S:Mn2+nanocrystals by using mercaptopropionic acid as stabilizer[J].Journal of Materials Chemistry,2003,13(7):1853-1857.
[24]Wu P,He Y,Wang H F,et al.Conjugation of glucose oxidase onto Mn-doped Zn S quantum dots for phosphorescent sensing of glucose in biological fluids[J].Analytical chemistry,2010,82(4):1427-1433.
[25]Zhuang J,Zhang X,Wang G,et al.Synthesis of water-soluble Zn S:Mn2+nanocrystals by using mercaptopropionic acid as stabilizer[J].Journal of Materials Chemistry,2003,13(7):1853-1857.
[26]Liu S P,Yang Z,Liu Z F,et al.Resonance Rayleigh scattering study on the interaction of gold nanoparticles with berberine hydrochloride and its analytical application[J].Analytica chimica acta,2006,572(2):283-289.
[27]闫曙光,刘绍璞,何佑秋.Cd Te/Zn S量子点荧光可逆调控研究ct DNA与吡柔比星的相互作用[J].科学通报,2011,56(22):1804-1811.
[28]Burda C,Green T C,Link S,et al.Electron shuttling across the interface of Cd Se nanoparticles monitored by femtosecond laser spectroscopy[J].The Journal of Physical Chemistry B,1999,103(11):1783-1788.
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