GC-MS分析新益心宁神片挥发性成分
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摘要
目的采用气相色谱-质谱法对新益心宁神片中挥发性成分进行研究。方法采用水蒸气蒸馏法提取新益心宁神片挥发油,GC-MS对所提取的挥发油成分进行分离鉴定,按照峰面积归一化法计算出各成分的相对百分含量。结果从新益心宁神片挥发油中共分离出47个色谱峰,鉴定出35个组分,占挥发油总量的93. 02%。其挥发油成分主要以萜类及其衍生物为主,萜类主要是倍半萜,衍生物主要是醇和酯,其中含量高于1%的成分有15种,主要包括依兰烯(30. 64%)、长叶蒎烯(10. 70%)、γ-雪松烯(9. 42%)、4-(1,5-二甲基-4-己烯亚基)-1-甲基环己烯(8. 24%)、α-柏木烯(6. 30%)、脱氢香橙烯(4. 13%)、4-叔丁基邻苯二酚-2甲醚(2. 54%)、β-雪松烯(2. 5%)、4-庚基苯乙酮(2. 15%)等。结论本方法适用于新益心宁神片挥发性成分分析,新益心宁神片挥发油种类丰富,能够为新益心宁神片的进一步开发利用研究提供理论依据。
Objective To Study volatile constituents in new Yixin Ningshen tablets by Gas Chromatography-Mass Spectrometry( GC-MS). Methods Volatile oil was extracted from new Yixin Ningshen Tablets by steam distillation. The volatile oil was separated and identified by GC-MS and the relative percentage of each component was calculated by peak area normalization method. Results47 chromatographic peaks were detected,among which 35 compounds were identified,accounting for 93. 02% of the total volatile oil.The main components were terpenoids and their derivatives,and terpenoids were mainly sesquiterpenes,and derivatives were alcohol and ester. There were 15 ingredients with a content of more than 1%,mainly including elane( 30. 64%),Longipinene( 10. 70%),γ-Cedrene( 9. 42%),4-( 1,5-dimethyl-4-hexene)-1-methylcyclohexene( 8. 24%),α-Cedrene( 6. 30%),Dehydroorangeene( 4. 13%),4-tert-butyl catechol-2 methyl ether( 2. 54%),β-Cedrene( 2. 5%),4-heptanyl Acetophenone( 2. 15%),etc. Conclusion The method is suitable for the analysis of volatile components in new Yixin Ningshen tablets. There are many kinds of volatile oil in new Yixin Ningshen tablets. The study provides a theoretical evidence for the further development and utilization of new Yixin Ningshen tablets.
Objective To Study volatile constituents in new Yixin Ningshen tablets by Gas Chromatography-Mass Spectrometry( GC-MS). Methods Volatile oil was extracted from new Yixin Ningshen Tablets by steam distillation. The volatile oil was separated and identified by GC-MS and the relative percentage of each component was calculated by peak area normalization method. Results47 chromatographic peaks were detected,among which 35 compounds were identified,accounting for 93. 02% of the total volatile oil.The main components were terpenoids and their derivatives,and terpenoids were mainly sesquiterpenes,and derivatives were alcohol and ester. There were 15 ingredients with a content of more than 1%,mainly including elane( 30. 64%),Longipinene( 10. 70%),γ-Cedrene( 9. 42%),4-( 1,5-dimethyl-4-hexene)-1-methylcyclohexene( 8. 24%),α-Cedrene( 6. 30%),Dehydroorangeene( 4. 13%),4-tert-butyl catechol-2 methyl ether( 2. 54%),β-Cedrene( 2. 5%),4-heptanyl Acetophenone( 2. 15%),etc. Conclusion The method is suitable for the analysis of volatile components in new Yixin Ningshen tablets. There are many kinds of volatile oil in new Yixin Ningshen tablets. The study provides a theoretical evidence for the further development and utilization of new Yixin Ningshen tablets.
引文
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[2]蔡晓琴,顾霞敏,唐小琴,等.合欢花总黄酮含量的测定及其超声提取工艺研究[J].安徽农业科学,2016,44(1):151-153.
[3]王爱梅,李旻,陈亚奇,等.合欢花水提物改善抑郁模型大鼠学习记忆机制的研究[J].国医论坛,2015,30(2):57-58.
[4]何玉贤,谭新,谭玫,等.合欢花与南蛇藤果实水煎剂镇静催眠作用的比较[J].中药材,2009,12(5):36-37.
[5]陶丽,刘延庆.南蛇藤多萜对4种化疗药物增效作用的实验研究[J].江苏中医药,2018,50(12):71-73.
[6]赵磊,贾玲玲,杨莉,等.南蛇藤茎中三萜类成分的分离及含量测定[J].解放军药学学报,2018,34(6):545-548.
[7]肖寄平.合欢花及其伪品的鉴别[J].长春中医药大学学报,2009,25(2):188-189.
[8]苏嘉焱,张锦锦,王程成,等.南蛇藤、鸡血藤配伍对佐剂性关节炎大鼠药效学与毒性的影响[J].中成药,2018,40(9):2068-2072.
[9]李铣.南蛇藤果实化学成分和药理活性研究[A].沈阳:沈阳药科大学,2004:77-80.
[10]万丽娟,卢金清,许俊洁,等.基于芳香性药物镇定安神的物质基础研究进展[J].中国药业,2015,24(15):1-3.
[11]孙震,赵振东,李冬梅,等.高纯度α-柏木烯的制备研究[J].林产化工通讯,2002,36(2):10-12.
[12]李晓光,叶富强,徐鸿华,等.砂仁挥发油中乙酸龙脑酯的药理作用研究[J].华西药学杂志,2001,16(5):356-358.
[13]陈旭兵,陈光勇.石竹烯的研究进展[J].山东化工,2011,40(7):34-36.
[14]张季林,魏惠珍,张洁.β-石竹烯生物学功能的研究进展[J].山东医药,2018,58(38):110-112.