Carbon Nanotubes Promote Interferon-γ Expression of T cell
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- 英文论文题名：Carbon Nanotubes Promote Interferon-γ Expression of T cell
- 论文作者：Dai Yali ; Meng Jie ; Xu haiyan
- 英文论文作者：Dai Yali ; Meng Jie ; Xu haiyan ; Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College
- 年：2016
- 作者机构：Institute of Basic Medical Sciences Chinese Academy of Medical Sciences School of Basic Medicine Peking Union Medical College;
- 英文论文关键词：carbon nanotube ; T cell ; polarization ; PMA
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：R730.51
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：2
- 文件大小：1335k
- 原文格式：D
- 会议级别：全国
摘要
Background: Carbon nanotubes are known to modulate a wide variety of cellular functions including inflammatory response [1]. Expression of interferon-γ(IFN-γ) in T lymphocytes is known to induce early inflammatory events and play important for evaluating toxicology of nanomaterials [2].Objectives: The study was aim to evaluate the effect of multiwalled carbon nanotubes(MWCNTs) to T cells and to explore the potential application of MWCNTs in antitumor immune therapy.Methods: We prepared oxidized MWCNTs, which was able to stably disperse in aqueous solution. The T cell morphology was observed by transmission electron microscopy(TEM). The reactive oxygen species production(ROS) was analyzed by flow cytometry. The calcium concentration was measured by confocal microscopy. The cell cytokine expression of T cells was studied by RT-PCR and ELISA. Results: Our results found that MWCNTs significantly enhanced reactive oxygen species production and increased calcium concentration in Jurkat T cells. The MWCNT inserted in the membrane of Jurkat T cell observed from the TEM. Interestingly, it was found that MWCNTs synergistic promoted Th1 type IFN-γ expression in Jurkat T cells activated by phorbol 12-myristate 13-acetate(PMA) without affecting cell viability. While Th2 type IL-10 expression were synergistic inhibited by MWCNT in activated T cells. Conclusion: These results suggest MWCNT synergizing PMA serve as a novel Th1 differentiation to regulate endogenous IFN-γ gene expression in Jurkat T cells.
Background: Carbon nanotubes are known to modulate a wide variety of cellular functions including inflammatory response [1]. Expression of interferon-γ(IFN-γ) in T lymphocytes is known to induce early inflammatory events and play important for evaluating toxicology of nanomaterials [2].Objectives: The study was aim to evaluate the effect of multiwalled carbon nanotubes(MWCNTs) to T cells and to explore the potential application of MWCNTs in antitumor immune therapy.Methods: We prepared oxidized MWCNTs, which was able to stably disperse in aqueous solution. The T cell morphology was observed by transmission electron microscopy(TEM). The reactive oxygen species production(ROS) was analyzed by flow cytometry. The calcium concentration was measured by confocal microscopy. The cell cytokine expression of T cells was studied by RT-PCR and ELISA. Results: Our results found that MWCNTs significantly enhanced reactive oxygen species production and increased calcium concentration in Jurkat T cells. The MWCNT inserted in the membrane of Jurkat T cell observed from the TEM. Interestingly, it was found that MWCNTs synergistic promoted Th1 type IFN-γ expression in Jurkat T cells activated by phorbol 12-myristate 13-acetate(PMA) without affecting cell viability. While Th2 type IL-10 expression were synergistic inhibited by MWCNT in activated T cells. Conclusion: These results suggest MWCNT synergizing PMA serve as a novel Th1 differentiation to regulate endogenous IFN-γ gene expression in Jurkat T cells.
Background: Carbon nanotubes are known to modulate a wide variety of cellular functions including inflammatory response [1]. Expression of interferon-γ(IFN-γ) in T lymphocytes is known to induce early inflammatory events and play important for evaluating toxicology of nanomaterials [2].Objectives: The study was aim to evaluate the effect of multiwalled carbon nanotubes(MWCNTs) to T cells and to explore the potential application of MWCNTs in antitumor immune therapy.Methods: We prepared oxidized MWCNTs, which was able to stably disperse in aqueous solution. The T cell morphology was observed by transmission electron microscopy(TEM). The reactive oxygen species production(ROS) was analyzed by flow cytometry. The calcium concentration was measured by confocal microscopy. The cell cytokine expression of T cells was studied by RT-PCR and ELISA. Results: Our results found that MWCNTs significantly enhanced reactive oxygen species production and increased calcium concentration in Jurkat T cells. The MWCNT inserted in the membrane of Jurkat T cell observed from the TEM. Interestingly, it was found that MWCNTs synergistic promoted Th1 type IFN-γ expression in Jurkat T cells activated by phorbol 12-myristate 13-acetate(PMA) without affecting cell viability. While Th2 type IL-10 expression were synergistic inhibited by MWCNT in activated T cells. Conclusion: These results suggest MWCNT synergizing PMA serve as a novel Th1 differentiation to regulate endogenous IFN-γ gene expression in Jurkat T cells.
引文
1.Meng J,Li X,Wang C,Guo H,Liu J,Xu H.ACS Appl Mater Interfaces.2015 Feb 11;7(5):3180-8.
2 .Hayashi K,Ishizuka S,Yokoyama C,Hatae T.Life Sci.2008 Jul 4;83(1-2):6-11.
2 .Hayashi K,Ishizuka S,Yokoyama C,Hatae T.Life Sci.2008 Jul 4;83(1-2):6-11.