微生物膜在有机毒性物质监测中的应激反应
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- 英文论文题名：Stress response of microbial biofilm during monitoring organic toxic substances
- 论文作者：刘玲 ; 高英 ; 曾佳佳 ; 董绍俊
- 英文论文作者：Ling Liu ; Ying Gao ; Jiajia Zeng ; Shaojun Dong ; State Key Laboratory of Electroanalytical Chemistry ; Changchun Institute of Applied Chemistry ; Chinese Academy of Sciences ; School of Science ; Changchun Institute of Technology
- 年：2016
- 作者机构：中国科学院长春应用化学研究所;长春工程学院理学院;
- 论文关键词：水体有机毒性检测 ; 微生物膜 ; 微生物膜应激反应
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十六分会：环境化学
- 语种：中文
- 分类号：X832;X172
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十六分会 ; 环境化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：2
- 文件大小：184k
- 原文格式：D
- 会议级别：全国
摘要
本研究以微生物呼吸作用消耗水中溶解氧为探针,以电化学还原溶解氧(DO)的电流变化表征水体中具有生物毒性有机物质的含量。有机毒性物质对微生物膜的影响可能存在多种情况:如毒性影响将使电化学信号降低;而有机毒性物质作为呼吸底物或微生物发生应激作用则将使信号升高。为了便于分析,我们以单一的有机毒性物质3’5-二氯苯酚(DCP)为研究对象。结果为负抑制(即促进呼吸,如图1所示)。但DCP又不是简单的作为代谢底物被微生物降解。因为如果是作为底物被微生物降解的情况,随着DCP浓度增加,负抑制应该增强,但实际的结果是随着DCP浓度的增加负抑制减弱。说明负抑制的出现是微生物的应激反应,是一种自我保护机制,而非利用DCP做为代谢底物。将DCP对微生物膜的抑制规律摸清有助于认识微生物毒性测试中有机毒性物质与有机底物的关系,以解决我们在毒性测试中经常遇到的出现负抑制,或者抑制规律混乱的问题[1,2]。
In this study,the consumption of dissolved oxygen by microbial respiration was used as a probe to characterize the organic toxicity in water.The electrochemical method was used for detecting the change of dissolve oxygen(DO).There are varieties of situations of organic toxic substances to microbial membrane:the toxicity effect will make the electrochemical signal decreased,but if organic toxic substrates will be used as respiration substances and the stress action of biofilm,which will make the signal increase.In the present study,we employed 3,5-dichlorophenol(DCP) as the sample.The result showed a negative inhibition(promote respiration,Figure 1).But DCP is not as simple as the metabolic substrate.If the DCP was used as the substrate degraded by microorganisms,the negative inhibition should be enhanced with the increase of the DCP concentration.But the present result showed a decrease with the increase of DCP concentration.The significance of this work is that helps us to rethink about the relationship between organic toxic substances and organic substrates of respiration [1,2].
In this study,the consumption of dissolved oxygen by microbial respiration was used as a probe to characterize the organic toxicity in water.The electrochemical method was used for detecting the change of dissolve oxygen(DO).There are varieties of situations of organic toxic substances to microbial membrane:the toxicity effect will make the electrochemical signal decreased,but if organic toxic substrates will be used as respiration substances and the stress action of biofilm,which will make the signal increase.In the present study,we employed 3,5-dichlorophenol(DCP) as the sample.The result showed a negative inhibition(promote respiration,Figure 1).But DCP is not as simple as the metabolic substrate.If the DCP was used as the substrate degraded by microorganisms,the negative inhibition should be enhanced with the increase of the DCP concentration.But the present result showed a decrease with the increase of DCP concentration.The significance of this work is that helps us to rethink about the relationship between organic toxic substances and organic substrates of respiration [1,2].
引文
[1]刘畅.基于铁氰化钾的微生物传感技术的研究,东北大学,2009.
[2]雍达明.基于人工媒介体的水体综合生物毒性测试方法的开发与应用,中国科学院大学,2013.
[2]雍达明.基于人工媒介体的水体综合生物毒性测试方法的开发与应用,中国科学院大学,2013.