利用层层组装技术和主客体相互作用构建生物分子识别表面
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- 英文论文题名：Buildup of Biomolecule Recognition Surface Based on Layer-by-Layer Assembly and Host-guest Interaction
- 论文作者：胡昌明 ; 渠阳翠 ; 占文俊 ; 韦婷 ; 曹利敏 ; 于谦 ; 陈红
- 英文论文作者：Changming Hu ; Yangcui Qu ; Wenjun Zhan ; Ting Wei ; Limin Cao ; Qian Yu ; Hong Chen ; College of Chemistry ; Chemical Engineering and Material Science ; Soochow University
- 年：2016
- 作者机构：苏州大学材料与化学化工学部;
- 论文关键词：层层组装 ; 主客体相互作用 ; 生物分子识别
- 英文论文关键词：layer-by-layer assembly ; host-guest interaction ; biomolecule recognition
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十四分会：超分子组装与软物质材料
- 语种：中文
- 分类号：O641.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十四分会 ; 超分子组装与软物质材料
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：207k
- 原文格式：D
- 会议级别：全国
摘要
具有生物分子识别能力的材料表面在生物传感器领域具有重要意义。在本工作中,我们结合层层自组装和主客体相互作用制备了一种能够高容量负载生物活性分子且具有良好生物分子识别能力的材料表面。首先在带有正电的硅片表面通过层层组装技术交替沉积了含金刚烷基团的聚丙烯酸共聚物和聚烯丙基胺,得到了含有金刚烷客体分子的聚电解质多层膜;而后利用主客体相互作用将生物分子修饰的主体分子β-环糊精引入到多层膜内。这里我们选择生物素-亲和素作为模型分子对考察表面的识别能力。荧光标记蛋白质吸附和石英晶体微天平的测试结果表明:引入了生物素修饰的β-环糊精的多层膜表面能够特异性地识别亲和素,同时对非特异性蛋白质保持了良好的排斥能力。不同亲和素浓度的吸附实验结果反映了该表面对目标分子检测的高灵敏度。该表面设计不仅局限于生物素-亲和素分子对,同时可以推广应用到多种生物分子识别体系,在生物检测领域具有潜在的应用前景。
Developing the surface with biomolecule recognition properties is of great interest for application of biosensor and biodetector. Herein, we provided a novel and universal method to fabricate biomolecule recognition surfaces with high biomolecule loading capability by the combination of layer-by-layer assembly and host-guest interaction. In detail, the positively charged silicon surface was alternately deposited with poly(acrylic acid-co-adamantane) and poly(allylamine hydrochlorich), followed by incorproration of a β-cyclodextrin derivative with biotin ligand via host-guest interaction. The successful preparation of this surface was comfirmed by adsorption of cyclodextrin labeled with fluorescence groups and monitored by using quartz crystal microbalance. The resultant surface exhibited high specific affinity of avidin but non-specific protein resistance. In addition, the high sensibility of the surface was demonstrated by the adsorption of avidin with different concentration. This system can be further applied in biological field by changing the biomolecules.
Developing the surface with biomolecule recognition properties is of great interest for application of biosensor and biodetector. Herein, we provided a novel and universal method to fabricate biomolecule recognition surfaces with high biomolecule loading capability by the combination of layer-by-layer assembly and host-guest interaction. In detail, the positively charged silicon surface was alternately deposited with poly(acrylic acid-co-adamantane) and poly(allylamine hydrochlorich), followed by incorproration of a β-cyclodextrin derivative with biotin ligand via host-guest interaction. The successful preparation of this surface was comfirmed by adsorption of cyclodextrin labeled with fluorescence groups and monitored by using quartz crystal microbalance. The resultant surface exhibited high specific affinity of avidin but non-specific protein resistance. In addition, the high sensibility of the surface was demonstrated by the adsorption of avidin with different concentration. This system can be further applied in biological field by changing the biomolecules.
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