健康奶牛阴道乳酸菌分离鉴定及作为候选益生菌株的体外评价
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- 英文论文题名：In vitro evaluation of candidate probiotics isolated from healthy dairy cow vaginas
- 论文作者：田丰松 ; 王军 ; 刘畅 ; 李长久 ; 丁赫 ; 杨雨江 ; 吕文发
- 英文论文作者：TIAN Feng-song ; WANG Jun ; LIU Chang ; LI Chang-jiu ; DING He ; YANG Yu-jiang ; LV Wen-fa ; College of Animal Science and Technology ; JiLin Agricultural University
- 年：2016
- 作者机构：吉林农业大学动物科学技术学院;
- 论文关键词：阴道 ; 乳酸菌 ; 抑菌活性
- 英文论文关键词：Vagina ; Lactobacillus ; Anti-bacteria activity
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S816.3
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：67k
- 原文格式：O
- 会议级别：全国
摘要
为分离健康奶牛阴道乳酸菌并鉴定其益生特性,本研究于采集的健康奶牛阴道临床样品中共分离筛选到4株乳酸菌,并对其抑菌活性、产酸性能、产H_2O_2能力及对抗生素敏感性进行检测,通过16S rDNA序列分析鉴定乳酸菌分离株。结果表明,4株乳酸菌具有较好的抑菌活性和产酸性能,在体外均能够产生H_2O_2,并对万古霉素具有抗性。经鉴定4株乳酸菌分别为唾液乳杆菌1株、黏膜乳杆菌2株、坚强肠球菌1株。4株乳酸菌均可以作为候选益生菌株用于奶牛子宫内膜炎防治。
This study aims to isolate probiotics from healthy dairy cow vaginas and evaluate the probiotic characterizations.Then four Lactobacillus were isolated from dairy cow vaginas,and the Lactobacillus isolates were tested for antibacterial activity,the acid and hydrogen peroxide producing abilities and antibiotics sensitivity.The results showed that all the isolates had the anti-bacteria activity,producting acid and hydrogen peroxide property and were also resistance to Vancomycin.According to the 16 S rDNA sequence identification,the 4 Lactobacillus isolates were 1L.salivarius,2 L.mucosae and 1 Enterococcus durans.The 4 Lactobacillus could be used as candidate probiotics to prevent and treat dairy cow endometritis.
This study aims to isolate probiotics from healthy dairy cow vaginas and evaluate the probiotic characterizations.Then four Lactobacillus were isolated from dairy cow vaginas,and the Lactobacillus isolates were tested for antibacterial activity,the acid and hydrogen peroxide producing abilities and antibiotics sensitivity.The results showed that all the isolates had the anti-bacteria activity,producting acid and hydrogen peroxide property and were also resistance to Vancomycin.According to the 16 S rDNA sequence identification,the 4 Lactobacillus isolates were 1L.salivarius,2 L.mucosae and 1 Enterococcus durans.The 4 Lactobacillus could be used as candidate probiotics to prevent and treat dairy cow endometritis.
引文
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[5].Parolin C,Marangoni A,Laghi L,et al.Isolation of Vaginal Lactobacilli and Characterization of Anti-Candida Activity[J].PloS one.2015;10(6):e0131220.
[2].Williams EJ,Fischer DP,Pfeiffer DU,et al.Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle[J].Theriogenology.2005;63(1):102-17.
[3].Mijac VD,Dukic SV,Opavski NZ,et al.Hydrogen peroxide producing lactobacilli in women with vaginal infections[J].EUR J OBSTET GYN R B.2006;129(1):69-76.
[4].Pendharkar S,Brandsborg E,Hammarstrom L,et al.Vaginal colonisation by probiotic lactobacilli and clinical outcome in women conventionally treated for bacterial vaginosis and yeast infection[J].BMC INFECT DIS.2015;15:255.
[5].Parolin C,Marangoni A,Laghi L,et al.Isolation of Vaginal Lactobacilli and Characterization of Anti-Candida Activity[J].PloS one.2015;10(6):e0131220.