静态三维校正与动态四维校正模型用于动态复杂体系中多目标物实时定量分析的比较研究
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- 英文论文题名：A Comparison of Real-time Quantitation in Complex Matrices Using Static Three-way Calibration and Dynamic Four-way Calibration Models
- 论文作者：尹小丽 ; 吴海龙 ; 谷惠文 ; 胡勇 ; 谢丽霞 ; 刘志 ; 王丽 ; 俞汝勤
- 英文论文作者：Xiao-Li Yin ; Hai-Long Wu ; Hui-Wen Gu ; Yong Hu ; Li-Xia Xie ; Zhi Liu ; Li Wang ; Ru-Qin Yu ; State Key Laboratory of Chemo/Biosensing and Chemometrics ; University of Hunan
- 年：2016
- 作者机构：湖南大学化学生物传感与计量学国家重点实验室;
- 论文关键词：静态三维校正模型 ; 动态四维校正模型 ; 近实时定量 ; 伊立替康 ; 二阶优势
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十五分会：化学信息学与化学计量学
- 语种：中文
- 分类号：TQ460.72
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十五分会 ; 化学信息学与化学计量学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：120k
- 原文格式：D
- 会议级别：全国
摘要
目前,三维校正和四维校正方法都可用于动力学过程的近实时定量分析。在实际应用中如何选择合适的校正方法成为了一个急需解决的问题。为此,本文以抗癌药物伊立替康(CPT-11)的水解动力学过程为例,分别建立静态三维校正和动态四维校正模型用于人血浆复杂体系中CPT-11水解动力学过程的近实时定量分析。研究发现,静态三维校正和动态四维校正模型均可以实现动力学过程中目标物的近实时定量分析。其中采用静态三维校正方法可以极大地减少实验的工作量,且静态三维校正方法更为灵活,可用于多种类型动力学过程的近实时定量分析。但它受限于校正曲线的校正浓度范围,在目标物实时浓度过低的情况下会导致预测结果不准确。而动态四维校正方法因在校正样中增加了动力学维度的信息,可以很好地弥补静态三维校正中这一缺点,然而四维动态校正方法实验工作量很大,且基于四线性分解模型的四维校正方法只能用于一级动力学过程的近实时定量分析,还有引入强共线性的风险。这一研究工作可以为选择合理方法进行动态复杂体系中目标物的近实时定量分析提供指导。
Both static three-way calibration and dynamic four-way calibration models were built for real-time quantitation of the hydrolysis of Irinotecan(CPT-11) in complex human plasma.Both the static three-way calibration and the dynamic four-way calibration models can achieve the real-time quantitative analysis.Three-way calibration method requires less experimental measurements;moreover,it is more flexible and suitable for a variety of dynamic process.However,the quantitation of three-way calibration model is limited by the concentration range of static calibration curve.Owing to the addition of kinetic information of calibration samples in dynamic four-way calibration model,the problem in three-way calibration model can be solved.Unfortunately,the experimental task in dynamic four-way calibration model is heavy;in addition,dynamic four-way calibration based on quadrilinear decomposition model can only be used in dealing with dynamic process that obey 1~(st)-order kinetics and at the risk of introducing colinearity.The comparison study in this work will provide a guidance for the reasonable selection of multi-way calibration methods to achieve the real-time quantitation of target analyte(s) in complex systems.
Both static three-way calibration and dynamic four-way calibration models were built for real-time quantitation of the hydrolysis of Irinotecan(CPT-11) in complex human plasma.Both the static three-way calibration and the dynamic four-way calibration models can achieve the real-time quantitative analysis.Three-way calibration method requires less experimental measurements;moreover,it is more flexible and suitable for a variety of dynamic process.However,the quantitation of three-way calibration model is limited by the concentration range of static calibration curve.Owing to the addition of kinetic information of calibration samples in dynamic four-way calibration model,the problem in three-way calibration model can be solved.Unfortunately,the experimental task in dynamic four-way calibration model is heavy;in addition,dynamic four-way calibration based on quadrilinear decomposition model can only be used in dealing with dynamic process that obey 1~(st)-order kinetics and at the risk of introducing colinearity.The comparison study in this work will provide a guidance for the reasonable selection of multi-way calibration methods to achieve the real-time quantitation of target analyte(s) in complex systems.
引文
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