BmICE-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, Bombyx mori
详细信息 查看官网全文
- 英文论文题名：BmICE-2 is a novel pro-apoptotic caspase involved in apoptosis in the silkworm, Bombyx mori
- 论文作者：Hua-Shan Yi ; Cai-Xia Pan ; Chun Pan ; Juan Song ; Yan-Fen Hu ; La Wang ; Min-Hui Pan ; Cheng Lu
- 英文论文作者：Hua-Shan Yi ; Cai-Xia Pan ; Chun Pan ; Juan Song ; Yan-Fen Hu ; La Wang ; Min-Hui Pan ; Cheng Lu ; State Key Laboratory of Silkworm Genome Biology ; Southwest University ; Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry ; Southwest University
- 年：2016
- 作者机构：State Key Laboratory of Silkworm Genome Biology, Southwest University;Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University;
- 英文论文关键词：Bombyx mori ; Apoptosis ; Caspase ; BmICE-2
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S881.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：498k
- 原文格式：D
- 会议级别：全国
摘要
In this study we identified a potential pro-apoptotic caspase gene, Bombyx mori(B. mori)ICE- 2(BmICE-2) which encoded a polypeptide of 284 amino acid residues, including a 169 QACRG 173 sequence which surrounded the catalytic site and contained a p20 and a p10 domain. BmICE-2 expressed in Escherichia coli(E. coli) exhibited high proteolytic activity for the synthetic human initiator caspase-9 substrates Ac-LEHD-pNA, but little activity towards the effector caspase-3 substra tes Ac-DEVD-pNA. When BmICE- 2 was transiently expressed in BmN-SWU1 silkworm B. mori cells, we found that the high proteolytic activity for Ac-LEHD-pNA triggered caspase-3-like protease activity resulting in spontaneous cleavage and apoptosis in these cells. This effect was not replicated in Spodoptera frugiperda 9 cells. In addition, spontaneous cleavage of endogenous BmICE-2 in BmN-SWU1 cells could be induced by actinomycin D. These results suggest that BmICE-2 may be a novel pro-apoptotic gene with caspase-9 activity which is involved apoptotic processes in BmN-SWU1 silkworm B. mori cells.
In this study we identified a potential pro-apoptotic caspase gene, Bombyx mori(B. mori)ICE- 2(BmICE-2) which encoded a polypeptide of 284 amino acid residues, including a 169 QACRG 173 sequence which surrounded the catalytic site and contained a p20 and a p10 domain. BmICE-2 expressed in Escherichia coli(E. coli) exhibited high proteolytic activity for the synthetic human initiator caspase-9 substrates Ac-LEHD-pNA, but little activity towards the effector caspase-3 substra tes Ac-DEVD-pNA. When BmICE- 2 was transiently expressed in BmN-SWU1 silkworm B. mori cells, we found that the high proteolytic activity for Ac-LEHD-pNA triggered caspase-3-like protease activity resulting in spontaneous cleavage and apoptosis in these cells. This effect was not replicated in Spodoptera frugiperda 9 cells. In addition, spontaneous cleavage of endogenous BmICE-2 in BmN-SWU1 cells could be induced by actinomycin D. These results suggest that BmICE-2 may be a novel pro-apoptotic gene with caspase-9 activity which is involved apoptotic processes in BmN-SWU1 silkworm B. mori cells.
In this study we identified a potential pro-apoptotic caspase gene, Bombyx mori(B. mori)ICE- 2(BmICE-2) which encoded a polypeptide of 284 amino acid residues, including a 169 QACRG 173 sequence which surrounded the catalytic site and contained a p20 and a p10 domain. BmICE-2 expressed in Escherichia coli(E. coli) exhibited high proteolytic activity for the synthetic human initiator caspase-9 substrates Ac-LEHD-pNA, but little activity towards the effector caspase-3 substra tes Ac-DEVD-pNA. When BmICE- 2 was transiently expressed in BmN-SWU1 silkworm B. mori cells, we found that the high proteolytic activity for Ac-LEHD-pNA triggered caspase-3-like protease activity resulting in spontaneous cleavage and apoptosis in these cells. This effect was not replicated in Spodoptera frugiperda 9 cells. In addition, spontaneous cleavage of endogenous BmICE-2 in BmN-SWU1 cells could be induced by actinomycin D. These results suggest that BmICE-2 may be a novel pro-apoptotic gene with caspase-9 activity which is involved apoptotic processes in BmN-SWU1 silkworm B. mori cells.
引文