A New Arylalkylamine N-Acetyltransferase in Silkworm(Bombyx mori) Affects Integument Pigmentation
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- 英文论文题名：A New Arylalkylamine N-Acetyltransferase in Silkworm(Bombyx mori) Affects Integument Pigmentation
- 论文作者：Yaohang Long ; Jiaorong Li ; Tianfu Zhao ; Guannan Li ; Yong Zhu
- 英文论文作者：Yaohang Long ; Jiaorong Li ; Tianfu Zhao ; Guannan Li ; Yong Zhu ; College of Biotechnology ; Southwest University
- 年：2016
- 作者机构：College of Biotechnology, Southwest University;
- 英文论文关键词：Arylalkylamine ; N-acetyltransferase(AANAT) ; Dopamine ; Melanin ; Transgenic silkworm ; Bombyx mori ; Integument color
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S881.2
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：498k
- 原文格式：D
- 会议级别：全国
摘要
Dopamine is a precursor for melanin synthesis. Arylalkylamine N-acetyltransferase(AANAT) is involved in the melatonin formation in insects because it could catalyze the transformation from dopamine to dopamine-N-acetyldopamine. In this study, we identified a new AANAT gene in the silkworm(Bombyx mori) and assessed its role in the silkworm. The c DNA of this gene encodes 233 amino acids that shares 57 % amino acid identity with the Bm-i AANAT protein. We thus refer to this gene as Bm-i AANAT2. To investigate the role of Bm-i AANAT2, we constructed a transgenic interference system using a 3xp3 promoter to suppress the expression of Bm-i AANAT2 in the silkworm. We observed that melanin deposition occurs in the head and integument in transgenic lines. To verify the melanism pattern, dopamine content and the enzyme activity of AANAT were determined by high-performance liquid chromatography(HPLC). We found that an increase in dopamine levels affects melanism patterns on the heads of transgenic B. mori. A reduction in the enzyme activity of AANAT leads to changes in dopamine levels. We analyzed the expression of the Bm-i AANAT2 genes by q PCR and found that the expression of Bm-i AANAT2 gene is significantly lower in transgenic lines. Our results lead us to conclude that Bm-i AANAT2 is a new arylalkylamine N-acetyl-transferase gene in the silkworm and is involved in the metabolism of the dopamine to avoid the generation of melanin.
Dopamine is a precursor for melanin synthesis. Arylalkylamine N-acetyltransferase(AANAT) is involved in the melatonin formation in insects because it could catalyze the transformation from dopamine to dopamine-N-acetyldopamine. In this study, we identified a new AANAT gene in the silkworm(Bombyx mori) and assessed its role in the silkworm. The c DNA of this gene encodes 233 amino acids that shares 57 % amino acid identity with the Bm-i AANAT protein. We thus refer to this gene as Bm-i AANAT2. To investigate the role of Bm-i AANAT2, we constructed a transgenic interference system using a 3xp3 promoter to suppress the expression of Bm-i AANAT2 in the silkworm. We observed that melanin deposition occurs in the head and integument in transgenic lines. To verify the melanism pattern, dopamine content and the enzyme activity of AANAT were determined by high-performance liquid chromatography(HPLC). We found that an increase in dopamine levels affects melanism patterns on the heads of transgenic B. mori. A reduction in the enzyme activity of AANAT leads to changes in dopamine levels. We analyzed the expression of the Bm-i AANAT2 genes by q PCR and found that the expression of Bm-i AANAT2 gene is significantly lower in transgenic lines. Our results lead us to conclude that Bm-i AANAT2 is a new arylalkylamine N-acetyl-transferase gene in the silkworm and is involved in the metabolism of the dopamine to avoid the generation of melanin.
Dopamine is a precursor for melanin synthesis. Arylalkylamine N-acetyltransferase(AANAT) is involved in the melatonin formation in insects because it could catalyze the transformation from dopamine to dopamine-N-acetyldopamine. In this study, we identified a new AANAT gene in the silkworm(Bombyx mori) and assessed its role in the silkworm. The c DNA of this gene encodes 233 amino acids that shares 57 % amino acid identity with the Bm-i AANAT protein. We thus refer to this gene as Bm-i AANAT2. To investigate the role of Bm-i AANAT2, we constructed a transgenic interference system using a 3xp3 promoter to suppress the expression of Bm-i AANAT2 in the silkworm. We observed that melanin deposition occurs in the head and integument in transgenic lines. To verify the melanism pattern, dopamine content and the enzyme activity of AANAT were determined by high-performance liquid chromatography(HPLC). We found that an increase in dopamine levels affects melanism patterns on the heads of transgenic B. mori. A reduction in the enzyme activity of AANAT leads to changes in dopamine levels. We analyzed the expression of the Bm-i AANAT2 genes by q PCR and found that the expression of Bm-i AANAT2 gene is significantly lower in transgenic lines. Our results lead us to conclude that Bm-i AANAT2 is a new arylalkylamine N-acetyl-transferase gene in the silkworm and is involved in the metabolism of the dopamine to avoid the generation of melanin.
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