Identification, Diversity and Evolution of MITEs in the Genomes of Microsporidian Nosema Parasites
详细信息 查看官网全文
- 英文论文题名：Identification, Diversity and Evolution of MITEs in the Genomes of Microsporidian Nosema Parasites
- 论文作者：何强 ; Zhenggang Ma ; Xiaoqun Dang ; 许金山 ; Zeyang Zhou
- 英文论文作者：Qiang He ; Zhenggang Ma ; Xiaoqun Dang ; Jinshan Xu ; Zeyang Zhou ; College of Life Sciences ; Chongqing Normal University ; State Key Laboratory of Silkworm Genome Biology ; Southwest University
- 年：2016
- 作者机构：College of Life Sciences, Chongqing Normal University;State Key Laboratory of Silkworm Genome Biology, Southwest University;
- 英文论文关键词：MITEs ; microsporidia ; evolution
- 会议召开时间：2016-07-01
- 会议录名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会论文集（摘要汇编）
- 语种：英文
- 分类号：S884
- 学会代码：ZGCU
- 会议名称：第十二届家（柞）蚕遗传育种暨良种繁育学术研讨会
- 会议地点：中国重庆
- 主办单位：中国蚕学会、国家蚕桑产业技术体系
- 学会名称：中国蚕学会
- 页数：1
- 文件大小：477k
- 原文格式：D
- 会议级别：全国
摘要
Miniature inverted-repeat transposable elements(MITEs) are short, non-autonomous DNA transposons, which are widespread in most eukaryotic genomes. However, genome-wide identification, origin and evolution of MITEs remain largely obscure in microsporidia. In this study, we investigated structural features for de novo identification of MITEs in genomes of silkworm microsporidia Nosema bombycis and Nosema antheraeae, as well as a honeybee microsporidia Nosema ceranae. A total of 1490, 149 and 83 MITE-related sequences from 89, 17 and five families, respectively, were found in the genomes of the above-mentioned species. Species-specific MITEs are predominant in each genome of microsporidian Nosema, with the exception of three MITE families that were shared by N. bombycis and N.antheraeae. One or multiple rounds of amplification occurred for MITEs in N. bombycis after divergence between N. bombyci sand the other two species, suggesting that the more abundant families in N. bombycis could be attribute d to the recent amplification of new MITEs. Significantly, some MITEs that inserted into the homologous protein-coding region of N. bombycis were recruited as introns, indicating that gene expansion occurred during the evolution of microsporidia. Nb S3 1and Nb S2 4had polymorphisms in diff erent geogra phi-cal strain s of N. bombycis, indicating that they could still be active. In addition, sever al small RNAs in the MITEs in N. bombycis are mainly produced from both ends of the MITEs sequence.
Miniature inverted-repeat transposable elements(MITEs) are short, non-autonomous DNA transposons, which are widespread in most eukaryotic genomes. However, genome-wide identification, origin and evolution of MITEs remain largely obscure in microsporidia. In this study, we investigated structural features for de novo identification of MITEs in genomes of silkworm microsporidia Nosema bombycis and Nosema antheraeae, as well as a honeybee microsporidia Nosema ceranae. A total of 1490, 149 and 83 MITE-related sequences from 89, 17 and five families, respectively, were found in the genomes of the above-mentioned species. Species-specific MITEs are predominant in each genome of microsporidian Nosema, with the exception of three MITE families that were shared by N. bombycis and N.antheraeae. One or multiple rounds of amplification occurred for MITEs in N. bombycis after divergence between N. bombyci sand the other two species, suggesting that the more abundant families in N. bombycis could be attribute d to the recent amplification of new MITEs. Significantly, some MITEs that inserted into the homologous protein-coding region of N. bombycis were recruited as introns, indicating that gene expansion occurred during the evolution of microsporidia. Nb S3 1and Nb S2 4had polymorphisms in diff erent geogra phi-cal strain s of N. bombycis, indicating that they could still be active. In addition, sever al small RNAs in the MITEs in N. bombycis are mainly produced from both ends of the MITEs sequence.
Miniature inverted-repeat transposable elements(MITEs) are short, non-autonomous DNA transposons, which are widespread in most eukaryotic genomes. However, genome-wide identification, origin and evolution of MITEs remain largely obscure in microsporidia. In this study, we investigated structural features for de novo identification of MITEs in genomes of silkworm microsporidia Nosema bombycis and Nosema antheraeae, as well as a honeybee microsporidia Nosema ceranae. A total of 1490, 149 and 83 MITE-related sequences from 89, 17 and five families, respectively, were found in the genomes of the above-mentioned species. Species-specific MITEs are predominant in each genome of microsporidian Nosema, with the exception of three MITE families that were shared by N. bombycis and N.antheraeae. One or multiple rounds of amplification occurred for MITEs in N. bombycis after divergence between N. bombyci sand the other two species, suggesting that the more abundant families in N. bombycis could be attribute d to the recent amplification of new MITEs. Significantly, some MITEs that inserted into the homologous protein-coding region of N. bombycis were recruited as introns, indicating that gene expansion occurred during the evolution of microsporidia. Nb S3 1and Nb S2 4had polymorphisms in diff erent geogra phi-cal strain s of N. bombycis, indicating that they could still be active. In addition, sever al small RNAs in the MITEs in N. bombycis are mainly produced from both ends of the MITEs sequence.
引文