GCRV免疫草鱼母本对子代早期发育中IgM、LSZ和C_3的表达影响
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- 英文论文题名：Effect of maternal GCRV immunization on the expression of IgM、LSZ and C_3 in Ctenopharyngodon idella offspring
- 论文作者：周智愚 ; 肖调义 ; 王荣华 ; 金生振 ; 汪小冬
- 英文论文作者：ZHOU Zhi-Yu ; XIAO Tiao-Yi ; WANG Rong-Hua ; JIN Sheng-Zhen ; WANG Xiao-Dong ; Hunan Engineering Technology Research Center of Featured Aquatic Resources Utilization ; Hunan Agricultural University ; Collaborative Innovation Center for Efficient and Health Production of Fisheries in Hunan Province
- 年：2016
- 作者机构：湖南省特色水产资源利用工程技术中心湖南农业大学;水产高效健康生产湖南省协调同创新中心;
- 论文关键词：草鱼 ; 母源免疫 ; 早期发育 ; 免疫因子 ; IgM
- 英文论文关键词：Ctenopharyngodon idella ; maternal immunity ; early development ; immune factor
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：S917.4
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：563k
- 原文格式：D
- 会议级别：全国
摘要
为探究体液免疫因子IgM、LSZ、C_3在草鱼代间的传递机制和免疫时效,本实验以草鱼出血病弱毒疫苗免疫产前40d草鱼母本,检测母本血液与子代早期发育阶段中3种免疫因子的基因表达和蛋白活性。Real-Time PCR结果表明,疫苗免疫后,母本血液中IgM、LSZ、C_3基因表达显著高于对照组(P<0.05)。实验组卵子中IgM(是对照组的2.4倍)与LSZ基因(是对照组的3.1倍)在卵子-12h(原肠胚期)表达水平急剧下降,24h(器官形成期)-3d(水花)检测不到表达,而C_3基因在24h(器官形成期)才开始出现表达。ELISA等结果表明,母本血液中3种免疫因子蛋白活性表达显著高于对照组(P<0.05),在胚胎和仔鱼期蛋白活性呈先下降后升高趋势,实验组IgM抗体水平在各发育时期显著高于对照组(P<0.05);LSZ和C_3蛋白活性在3d(水花)以后,与对照组无显著差异。2月龄鱼种实验组IgM基因在免疫组织(脾、肾、头肾)表达量均显著上调。研究显示,通过GCRV疫苗免疫能提高草鱼母源性免疫因子由亲本向子代传递能力,并对子代早期发育起免疫保护作用。
In order to explore the transfer mechanismand chronergyof humoral immune factors IgM, LSZ and C_3 between generations in Grass Carp,we vaccinated the broodstocks of the fish which was 40 d before antenatal with GCRV attenuated vaccine todetect gene expression and protein activityof parental blood and early developmental stages. The results of Real-Time PCR showed Grass Carp maternal blood IgM, LSZ and C_3 gene expression was significantly higher than the control group(P<0.05)after vaccination. The level of IgM geneand LSZ gene's expression during gastrulation was decreased rapidly,organ formation period-Fry was not detected expression,C_3 gene's expression duringorgan formation period began appearing. The ELISA indicated maternal blood 3 immune factors protein expression was significantly higher than the control group(P<0.05), The activities of protein in embryos and larvae Showed a trend from decline to rise.
In order to explore the transfer mechanismand chronergyof humoral immune factors IgM, LSZ and C_3 between generations in Grass Carp,we vaccinated the broodstocks of the fish which was 40 d before antenatal with GCRV attenuated vaccine todetect gene expression and protein activityof parental blood and early developmental stages. The results of Real-Time PCR showed Grass Carp maternal blood IgM, LSZ and C_3 gene expression was significantly higher than the control group(P<0.05)after vaccination. The level of IgM geneand LSZ gene's expression during gastrulation was decreased rapidly,organ formation period-Fry was not detected expression,C_3 gene's expression duringorgan formation period began appearing. The ELISA indicated maternal blood 3 immune factors protein expression was significantly higher than the control group(P<0.05), The activities of protein in embryos and larvae Showed a trend from decline to rise.
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