免标记策略发展富勒烯基纳米荧光探针
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- 英文论文题名：Fullerene-based Fluorescent Probe: Preparation by Label-free Strategy
- 论文作者：熊凤霞 ; 刘富洋 ; 范宜康 ; 邰付菊 ; 王合中 ; 何睿
- 英文论文作者：Xiong F. ; Liu F. ; Fan Y. ; Tai F. ; Wang H. ; He R. ; Nano Agro Center ; Henan Agricultural University ; College of Life Science ; Henan Agricultural University
- 年：2016
- 作者机构：河南农业大学植物保护学院/农业纳米研究中心;河南农业大学生命科学学院;
- 论文关键词：免标记 ; 富勒烯 ; 氮宾反应 ; 荧光探针
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十八分会：纳米生物效应与纳米药物化学
- 语种：中文
- 分类号：O657.3
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十八分会 ; 纳米生物效应与纳米药物化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：219k
- 原文格式：D
- 会议级别：全国
摘要
基于富勒烯水溶性衍生物组建多模态分子影像探针,结合抗肿瘤药物,可实现恶性肿瘤的早期诊断及治疗,这是刚刚新兴的纳米诊疗技术研究新亮点。当前,利用免标记策略发展富勒烯基纳米荧光探针的报道尚不多见~([1])。本文采用前期工作中发展的[2+1]环加成氮宾反应法~([2]),由叠氮乙胺(N_3CH_2CH_2NH_2)与富勒烯C_(60)经多加成反应制备出加成数目在极窄范围分布的C_(60)多氮杂桥水溶性衍生物,即氮杂富勒胺,TGA测试平均分子式为C_(60)(NCH_2CH_2NH_2)_(10)(Fig.1a)。荧光光谱测试显示新合成的水溶性氮杂富勒胺能受激产生荧光(Fig.1b)。这意味着利用氮宾反应发展新型富勒烯多氮杂桥水溶性衍生物,免标记荧光自身即可作潜在的纳米荧光探针。这为免标记策略发展富勒烯基多模态分子影像探针乃至多功能纳米诊疗试剂奠定坚实基础。
The water-soluble fullerene-based materials have attracted considerable attentions as the new generation of theranostic agents in nanomedicine.We used multiple additions of azides to produce the water-soluble multiazafulleroid derivative by nitrene chemistry ~([2]) with adding 50 equivalents of azidoethylamine(N_3CH_2CH_2NH_2)into the 1,2-dichlorobenzene solution of C_(60) at 180℃ for 24 hours,which number of added groups was estimated by simultaneous TG-DTG-DSC analysis(Fig.1a).The average molecular formula could be written as C_(60)(NCH_2CH_2NH_2)_(10).The emission spectrum of C_(60)(NCH_2CH_2NH_2)_(10) in solution under UV irradiation showed strong fluorescence(Fig.1b).Although the exact mechanism for the generation of fluorescence is unclear~([1]),the preliminary results indicated that the water-soluble multiazafulleroid derivatives have potential applications as fluorescent nanoprobes by label-free strategy.~1
The water-soluble fullerene-based materials have attracted considerable attentions as the new generation of theranostic agents in nanomedicine.We used multiple additions of azides to produce the water-soluble multiazafulleroid derivative by nitrene chemistry ~([2]) with adding 50 equivalents of azidoethylamine(N_3CH_2CH_2NH_2)into the 1,2-dichlorobenzene solution of C_(60) at 180℃ for 24 hours,which number of added groups was estimated by simultaneous TG-DTG-DSC analysis(Fig.1a).The average molecular formula could be written as C_(60)(NCH_2CH_2NH_2)_(10).The emission spectrum of C_(60)(NCH_2CH_2NH_2)_(10) in solution under UV irradiation showed strong fluorescence(Fig.1b).Although the exact mechanism for the generation of fluorescence is unclear~([1]),the preliminary results indicated that the water-soluble multiazafulleroid derivatives have potential applications as fluorescent nanoprobes by label-free strategy.~1
引文
[1]Zheng J.,et al.Carbon.2013,65:175.
[2]Xiong F.,et al.Nanomed-Nanotechnol.2016,12(2):525.
[2]Xiong F.,et al.Nanomed-Nanotechnol.2016,12(2):525.