纳米水凝胶负载基于BODIPY的荧光探针用于钙离子检测
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- 英文论文题名：Nanogel-Incorporating Fluorescent Probe Based on BODIPY for Calcium Ion Detection
- 论文作者：郭旭东 ; 杨国强
- 英文论文作者：Xudong Guo ; Guoqiang Yang ; Institute of Chemistry ; Chinese Academy of Sciences
- 年：2016
- 作者机构：中国科学院化学研究所;
- 论文关键词：荧光探针 ; 钙离子检测 ; BODIPY
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十七分会：光化学
- 语种：中文
- 分类号：O657.3;O648.17
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十七分会 ; 光化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：204k
- 原文格式：D
- 会议级别：全国
摘要
钙离子在生命体系中起着重要的作用。生物体内钙离子含量与多种生物过程密切相关,如神经兴奋、神经递质的释放、基因转录、细胞增殖和激素分泌等。使用荧光法检测钙离子,具有灵敏度高、响应迅速、易于可视化检测等优点,且可用于生物体成像,因此受到广泛关注。本工作设计了一种基于BODIPY衍生物荧光团和钙离子特异基团1,2-双(2-氨基苯氧基)乙烷-N,N,N',N'-四乙酸(BAPTA)的油溶性分子,该分子与钙离子反应之后,光致电子转移效应消失,原本被猝灭的发光重新显现,故可作为荧光发光型钙离子探针。使用纳米水凝胶负载该分子制备荧光纳米器件,可灵敏、高效、准确地检测水体系中的钙离子含量。
Calcium ions play vital role in organism. The level of calcium ions is sensitive to many biological processes, such as neural excitation, release of neurotransmitter and cell proliferation. Herein, a fluorescent probe for calcium ion detection was reported. The probe, which consisted of a BODIPY-derived fluorophore and a calcium-specific 1,2-bis(O-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid(BAPTA) moiety, could recuperate its fluorescence upon the calcium ions addition because the photon-induced electron transfer process was exhibited. After incorporated with nanogel, the water-insoluble probe could be applied in aqueous system with fast response, high sensitivity and selectivity to calcium ions.
Calcium ions play vital role in organism. The level of calcium ions is sensitive to many biological processes, such as neural excitation, release of neurotransmitter and cell proliferation. Herein, a fluorescent probe for calcium ion detection was reported. The probe, which consisted of a BODIPY-derived fluorophore and a calcium-specific 1,2-bis(O-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid(BAPTA) moiety, could recuperate its fluorescence upon the calcium ions addition because the photon-induced electron transfer process was exhibited. After incorporated with nanogel, the water-insoluble probe could be applied in aqueous system with fast response, high sensitivity and selectivity to calcium ions.
引文
[1]Chen,Y;Zhao,J;Guo,H;Xie,L.J.Org.Chem.2012,77,2192.
[2]Kamiya,M;Johnsson,K.Anal.Chem.2010,82,6472.
[2]Kamiya,M;Johnsson,K.Anal.Chem.2010,82,6472.