一种基于等离子体共振增强组装体检测危害因子的通用方法
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- 英文论文题名：A general approach for detecting hazard factors with aptamer-induced plasmonic-enhanced complex
- 论文作者：赵森 ; 曹文博 ; 吴世嘉 ; 王周平
- 英文论文作者：Zhao Sen ; Cao Wenbo ; Wu Shijia ; Wang Zhouping ; Synergetic Innovation Center of Food Safety and Nutrition ; School of Food Science and Technology ; Jiangnan University ; State Key Laboratory of Food Science and Technology ; Jiangnan University
- 年：2016
- 作者机构：江南大学食品学院;江南大学国家重点实验室;
- 论文关键词：荧光增强 ; 上转换 ; 食品危害因子 ; 组装
- 英文论文关键词：upconversion ; hazard factors ; assembly ; enhanced luminescence ; aptamer
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS207.3;O657.3
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：106k
- 原文格式：O
- 会议级别：全国
摘要
上转换纳米材料具有反Stokes发光特性,是基于通过双光子或多光子机制把长波辐射转换成短波辐射的荧光材料。因其发光亮度高、斯托克斯位移大、化学稳定性好、灵敏度高、无背景荧光、生物相容性好而被认为是一种理想的荧光探针。上转换纳米粒子作为最新一代荧光标记已有广泛的应用研究,例如在临床医学、药物筛选、食品安全等方面得到了广泛的应用研究,特别在生物分子检测、组织细胞成像、光热治疗方面的应用尤为突出。本文首先利用单链DNA的易修饰、易组装特性,将上转换纳米粒子和金纳米颗粒进行组装,通过调节DNA碱基数目来控制粒子间距,利用金纳米粒子的等离子体共振效应在粒子间距最优条件下实现对上转换特定波长荧光发射强度的增强。在此模式下,以特异性的适配体替换链DNA进行复合体的组装,从而实现对食品中危害因子的超灵敏检测。该检测模式的建立,借助于贵金属的等离子体共振效应充分利用了上转换材料的荧光探针优势,实现了不同目标物的单模式快速灵敏检测。
Lanthanide-doped upconversion nanoparticles(UCNPs) have emerged as alternatives over conventional probes because of their unique ability to generate shorter wavelength emissions under longer wavelength excitations.This anti-Stokes luminescence process requires luminescent center to absorb the energy of two or multiple photons.Currently,UCNP has been considered as a perfect luminescent probe with high brightness,sharp emission bandwidths,large anti-Stokes shifts,high detection sensitivity,weak background luminescence and good biocompatibility.Precisely,it has been used widely in the field of clinical medicine,drug screening and food safety,especially in detecting biomolecule,cell or tissue imaging and photothermal therapy.In this work,UCNPs-gold nanoparticle complex was fabricated using single strand DNA.By tuning the gaps between UCNPs and gold nanoparticles,we obtained the expected luminescence signal enhanced by the plasmonic property of small gold nanoparticles.Based on the developed assembly approach,by replacing the single strand DNA with aptamer,variety of hazard factors in food could be detected sensitively with corresponding aptamer.This integrated complex shows great potential of the future application in food safety.
Lanthanide-doped upconversion nanoparticles(UCNPs) have emerged as alternatives over conventional probes because of their unique ability to generate shorter wavelength emissions under longer wavelength excitations.This anti-Stokes luminescence process requires luminescent center to absorb the energy of two or multiple photons.Currently,UCNP has been considered as a perfect luminescent probe with high brightness,sharp emission bandwidths,large anti-Stokes shifts,high detection sensitivity,weak background luminescence and good biocompatibility.Precisely,it has been used widely in the field of clinical medicine,drug screening and food safety,especially in detecting biomolecule,cell or tissue imaging and photothermal therapy.In this work,UCNPs-gold nanoparticle complex was fabricated using single strand DNA.By tuning the gaps between UCNPs and gold nanoparticles,we obtained the expected luminescence signal enhanced by the plasmonic property of small gold nanoparticles.Based on the developed assembly approach,by replacing the single strand DNA with aptamer,variety of hazard factors in food could be detected sensitively with corresponding aptamer.This integrated complex shows great potential of the future application in food safety.
引文