基于高通量测序的废次烟末水提液中细菌群落分析
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摘要
为了解造纸法再造烟叶生产过程中不同烟末提取液的微生物变化,利用Roche 454 FLX焦磷酸高通量测序法,比较废次烟末水提液中细菌16S r RNA基因序列的多样性,获得了废次烟末水提液中细菌种群的组成和丰度的变化。结果表明:在废次烟末水提液的原液(M1)、浓缩液(M2)及醇化液(M3)样品中,细菌种群组成非常丰富,其中占主导地位的细菌种群是厚壁菌门,而厚壁菌门中的优势菌属是乳杆菌属和赖氨酸芽孢杆菌属。同时发现,从M1到M3的过程中,乳杆菌属丰度呈下降趋势,而赖氨酸芽孢杆菌属丰度呈上升趋势。基于高通量测序的细菌群落变化规律,为改善造纸法再造烟叶的品质提供了科学依据。
引文
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[10]Yuan Y J,Lu Z X,Huang L J,et al.Biodegradation of nicotine from tobacco waste extract by Ochrobactrum intermedium DN2[J].Journal of Industrial Microbiology&Biotechnology,2007,34(8):567-570.
[11]Palliyarakkal M K,Ramaswamy M,Vadivel A.Microsatellites in palm(Arecaceae)sequences[J].Bioinformation,2011,7(7):347-351.
[12]Schloss P D,Westcott S L,Ryabin T,et al.Introducing mothur:open-source,platform-independent,community-supported software for describing and comparing microbial communities[J].Applied and Environmental Microbiology,2009,75(23):7537-7541.
[13]Yarza P,Richter M,Peplies J,et al.The All-Species Living Tree project:A 16S r RNA-based phylogenetic tree of all sequenced type strains[J].Systematic and Applied Microbiology,2008,31(4):241-250.
[14]Omran M G H,Engelbrecht A P,Salman A.An overview of clustering methods[J].Intelligent Data Analysis,2007,11(6):583-605.
[15]Wei Y M,Wang J Q,Liu T T,et al.Bacterial communities of Beijing surface waters as revealed by 454 pyrosequencing of the 16S r RNA gene[J].Environmental Science and Pollution Research,2015,22(16):12605-12614.
[16]Dan D,Zhang D P,Liu W C,et al.Diversity analysis of bacterial community from permafrost soil of Mo-he in China[J].Indian Journal of Microbiology,2014,54(1):111-113.
[17]Chao A,Shen T J.Nonparametric estimation of Shannon's index of diversity when there are unseen species in sample[J].Environmental and Ecological Statistics,2003,10(4):429-443.
[18]Hunter P R,Gaston M A.Numerical index of the discriminatory ability of typing systems:an application of Simpson's index of diversity[J].Journal of Clinical Microbiology,1988,26(11):2465-2466.
[19]Huang J W,Yang J K,Duan Y Q,et al.Bacterial diversities on unaged and aging flue-cured tobacco leaves estimated by 16S r RNA sequence analysis[J].Applied Microbiology and Biotechnology,2010,88(2):553-562.
[20]Su C,Gu W,Zhe W,et al.Diversity and phylogeny of bacteria on Zimbabwe tobacco leaves estimated by 16S r RNA sequence analysis[J].Applied Microbiology and Biotechnology,2011,92(5):1033-1044.
[21]陈泽斌,夏振远,雷丽萍,等.烟草可培养内生细菌的分离及多样性分析[J].微生物学通报,2011,38(9):1347-1354.CHEN Zebin,XIA Zhenyuan,LEI Liping,et al.Diversity of cultivable endophytic bacteria isolated from tobacco[J].Microbiology China,2011,38(9):1347-1354.
[22]Chen C M,Li X M,Yang J K,et al.Isolation of nicotine-degrading bacterium Pseudomonas sp.Nic22,and its potential application in tobacco processing[J].International Biodeterioration&Biodegradation,2008,62(3):226-231.
[23]Li H J,Li X M,Duan Y Q,et al.Biotransformation of nicotine by microorganism:the case of Pseudomonas spp.[J].Applied Microbiology and Biotechnology,2010,86(1):11-17.
[24]Ruan A D,Min H,Peng X H,et al.Isolation and characterization of Pseudomonas sp.strain HF-1,capable of degrading nicotine[J].Research in Microbiology,2005,156(5-6):700-706.