Analysis of the Interaction of Endogenous Retrovirus ev21 and Avian Leukosis Virus Subgroup J in Late-feathering Chicken
详细信息 查看官网全文
- 英文论文题名：Analysis of the Interaction of Endogenous Retrovirus ev21 and Avian Leukosis Virus Subgroup J in Late-feathering Chicken
- 论文作者：冯敏 ; Zhang xiquan
- 英文论文作者：Zhang xiquan ; College of Animal Science ; South China Agricultural University
- 年：2016
- 作者机构：College of Animal Science,South China Agricultural University;
- 英文论文关键词：ev21 ; late feathering chicken ; ALV-J ; ISGs ; envelope protein
- 会议召开时间：2016-11-04
- 会议录名称：第十一届全国免疫学学术大会摘要汇编
- 英文会议录名称：Abstracts of 2016 CSI Congress on Immunology
- 语种：英文
- 分类号：S852.65
- 学会代码：IGMD
- 会议名称：第十一届全国免疫学学术大会
- 会议地点：中国安徽合肥
- 主办单位：中国免疫学会
- 学会名称：中国免疫学会
- 页数：1
- 文件大小：782k
- 原文格式：D
- 会议级别：全国
摘要
Avian leukosis virus subgroup J(ALV-J) infection can cause tumors and immunosuppression. Endogenous viruses integrate into host genomes and can recombine with exogenous avian leukosis virus(ALV). In this study, we analyzed the interaction of endogenous retrovirus 21(ev21) with the ALV-J in late-feathering Chinese yellow chicken. Two ALV-J strains M180 and K243 were isolated from late-feathering and fast-feathering Chinese yellow chicken flocks, respectively. The env gene of the two strains showed 94.2-94.8% nucleotide identity with reference ALV-J strains. Compared with the env gene and the LTR of ev21 and M180, the nucleotide identity of LTR was 69.7% and env gene was 58.4%, respectively, especially the amino acid identity of env gene as low as 14.2%. Phylogenetic analysis of the nucleotide sequence of the env gene and the 3'LTR showed that M180 was closely related to ALV-J, and was located in a distinct group with ev21 in the phylogenetic tree. Using co-immunoprecipitation(co-IP), we next demonstrate that the envelope protein of ev21 does not interact with the M180 envelope protein. We further show that the envelope protein of ev21 cannot activate ALV-J LTR promoter activity using luciferase-reporter assays. qPCR and western blot analysis revealed that envelope protein of endogenous ev21 can facilitate the expression of PKR at 6h post ALV-J infection(hpi) and facilitate the expression of ISG12 and CH25 H at 24 hpi. However, the expression of the env gene of M180 strain was not significantly at 6 hpi and 24 hpi. We found no evidence of recombination between ALV-J strain M180 and the endogenous retrovirus ev21 in late-feathering Chinese yellow chicken. The envelope protein of ev21 can affect the expression of host ISGs, but appears not to influence the replication of ALV-J strain M180.
Avian leukosis virus subgroup J(ALV-J) infection can cause tumors and immunosuppression. Endogenous viruses integrate into host genomes and can recombine with exogenous avian leukosis virus(ALV). In this study, we analyzed the interaction of endogenous retrovirus 21(ev21) with the ALV-J in late-feathering Chinese yellow chicken. Two ALV-J strains M180 and K243 were isolated from late-feathering and fast-feathering Chinese yellow chicken flocks, respectively. The env gene of the two strains showed 94.2-94.8% nucleotide identity with reference ALV-J strains. Compared with the env gene and the LTR of ev21 and M180, the nucleotide identity of LTR was 69.7% and env gene was 58.4%, respectively, especially the amino acid identity of env gene as low as 14.2%. Phylogenetic analysis of the nucleotide sequence of the env gene and the 3'LTR showed that M180 was closely related to ALV-J, and was located in a distinct group with ev21 in the phylogenetic tree. Using co-immunoprecipitation(co-IP), we next demonstrate that the envelope protein of ev21 does not interact with the M180 envelope protein. We further show that the envelope protein of ev21 cannot activate ALV-J LTR promoter activity using luciferase-reporter assays. q PCR and western blot analysis revealed that envelope protein of endogenous ev21 can facilitate the expression of PKR at 6h post ALV-J infection(hpi) and facilitate the expression of ISG12 and CH25 H at 24 hpi. However, the expression of the env gene of M180 strain was not significantly at 6 hpi and 24 hpi. We found no evidence of recombination between ALV-J strain M180 and the endogenous retrovirus ev21 in late-feathering Chinese yellow chicken. The envelope protein of ev21 can affect the expression of host ISGs, but appears not to influence the replication of ALV-J strain M180.
Avian leukosis virus subgroup J(ALV-J) infection can cause tumors and immunosuppression. Endogenous viruses integrate into host genomes and can recombine with exogenous avian leukosis virus(ALV). In this study, we analyzed the interaction of endogenous retrovirus 21(ev21) with the ALV-J in late-feathering Chinese yellow chicken. Two ALV-J strains M180 and K243 were isolated from late-feathering and fast-feathering Chinese yellow chicken flocks, respectively. The env gene of the two strains showed 94.2-94.8% nucleotide identity with reference ALV-J strains. Compared with the env gene and the LTR of ev21 and M180, the nucleotide identity of LTR was 69.7% and env gene was 58.4%, respectively, especially the amino acid identity of env gene as low as 14.2%. Phylogenetic analysis of the nucleotide sequence of the env gene and the 3'LTR showed that M180 was closely related to ALV-J, and was located in a distinct group with ev21 in the phylogenetic tree. Using co-immunoprecipitation(co-IP), we next demonstrate that the envelope protein of ev21 does not interact with the M180 envelope protein. We further show that the envelope protein of ev21 cannot activate ALV-J LTR promoter activity using luciferase-reporter assays. q PCR and western blot analysis revealed that envelope protein of endogenous ev21 can facilitate the expression of PKR at 6h post ALV-J infection(hpi) and facilitate the expression of ISG12 and CH25 H at 24 hpi. However, the expression of the env gene of M180 strain was not significantly at 6 hpi and 24 hpi. We found no evidence of recombination between ALV-J strain M180 and the endogenous retrovirus ev21 in late-feathering Chinese yellow chicken. The envelope protein of ev21 can affect the expression of host ISGs, but appears not to influence the replication of ALV-J strain M180.
引文