分子量对海参岩藻聚糖硫酸酯空间构象及胃粘膜保护功能的影响
详细信息 查看官网全文
- 英文论文题名：The influence of molecular mass on the chain conformation and gastric protective effects of sea cucumber fucoidan
- 论文作者：常耀光 ; 续晓琪 ; 薛长湖
- 英文论文作者：Yaoguang Chang ; Xiaoqi Xu ; Changhu Xue ; College of Food Science and Engineering ; Ocean University of China
- 年：2016
- 作者机构：中国海洋大学食品科学与工程学院;
- 论文关键词：岩藻聚糖硫酸酯 ; 海参 ; 胃粘膜保护 ; 分子量 ; 空间构象
- 英文论文关键词：fucoidan ; sea cucumber ; gastric protective effect ; molecular mass ; conformation
- 会议召开时间：2016-11-02
- 会议录名称：2016年中国水产学会学术年会论文摘要集
- 语种：中文
- 分类号：TS254.1
- 学会代码：OGSB
- 会议名称：2016年中国水产学会学术年会
- 会议地点：中国四川成都
- 主办单位：中国水产学会、四川省水产学会
- 学会名称：中国水产学会
- 页数：1
- 文件大小：537k
- 原文格式：D
- 会议级别：全国
摘要
海参岩藻聚糖硫酸酯是海参体壁中主要的活性成分之一,已被证实具有多种生理调节功能,在功能食品开发方面展现出良好的应用前景,然而关于其的构效关系的研究仍鲜见报道。本研究以一级结构明确的梅花参岩藻聚糖硫酸酯(1379.7 kDa)为实验材料,酶法制备系列分子量海参岩藻聚糖硫酸酯降解产物(795.0 kDa、556.2 kDa、112.8 kDa)。利用在线多角度激光光散射及原子力显微镜分析四种多糖样品的空间构象,以大鼠乙醇胃损伤模型考察不同多糖的胃粘膜保护功能,并通过组织切片、Western blotting及qRT-PCR等技术探究功能机理。研究表明,随着分子量的降低,海参岩藻聚糖硫酸酯的分子尺寸逐渐减小,多糖空间构象由半刚性链转变为无规则卷曲链并最终转变为球形;各样品均能显著抑制乙醇型胃溃疡的发生,功能机理包括抗氧化及缓解炎症等多个方面;其胃粘膜保护功能与其分子量之间存在非线性的构效关系:当多糖呈链状时,溃疡抑制率随分子量及分子尺寸的降低逐渐降低;但当分子量继续降低,链构象由无规则卷曲链向球形的转变将带来溃疡抑制率的显著提高。
Using fucoidan from sea cucumber Thelenota ananas(1379.7 kDa) and fucoidanase,fucoidan degradation products with different a series of molecular mass(795.0 kDa, 556.2 kDa,112.8 kDa) were prepared. The chain conformation of those fucoidan samples was analyzed by using HPSEC-MALLS-Vis-RID and atomic force microscopy, the gastric protective effects were examined by ethanol-induced rat gastric ulcer model. With the decreasing of molecular mass, the chain size of sea cucumber fucoidan decreased, and chain conformation converted form semi-flexible coil to random coil and then to sphere. All examined samples could significantly inhibit the gastric ulcer induced by ethanol, which could be attributed to various mechanisms including anti-oxidant and anti-inflammatory functions. The relationship of the protective effect and molecular mass was non-linear: when fucoidans adopted coil conformations, their effects decreased with the decreasing of molecular mass and size; while the transformation of chain conformation from coil to sphere brought a significant increase in the effect.
Using fucoidan from sea cucumber Thelenota ananas(1379.7 kDa) and fucoidanase,fucoidan degradation products with different a series of molecular mass(795.0 kDa, 556.2 kDa,112.8 kDa) were prepared. The chain conformation of those fucoidan samples was analyzed by using HPSEC-MALLS-Vis-RID and atomic force microscopy, the gastric protective effects were examined by ethanol-induced rat gastric ulcer model. With the decreasing of molecular mass, the chain size of sea cucumber fucoidan decreased, and chain conformation converted form semi-flexible coil to random coil and then to sphere. All examined samples could significantly inhibit the gastric ulcer induced by ethanol, which could be attributed to various mechanisms including anti-oxidant and anti-inflammatory functions. The relationship of the protective effect and molecular mass was non-linear: when fucoidans adopted coil conformations, their effects decreased with the decreasing of molecular mass and size; while the transformation of chain conformation from coil to sphere brought a significant increase in the effect.
引文