大豆分离蛋白制备硒化多肽复合物及其结构、性质研究
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- 英文论文题名：Study on preparation,structure and activity of soybean protein isolate peptide-se complex
- 论文作者：张馨元 ; 汪少芸
- 英文论文作者：Zhang Xinyuan ; Wang Shaoyun ; College of Biological Science and Technology ; Fuzhou University
- 年：2016
- 作者机构：福州大学生物科学与工程学院;
- 论文关键词：大豆分离蛋白 ; 活性肽 ; 螯合 ; 硒 ; 抗氧化
- 英文论文关键词：soybean protein isolate ; bioactive peptides ; chelation ; Se ; antioxidant
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS201.2
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：1
- 文件大小：46k
- 原文格式：O
- 会议级别：全国
摘要
本研究利用响应面法,以较高硒螯合力为指标,得到以复合风味蛋白酶酶解大豆分离蛋白的最优工艺为酶解温度50℃、底物浓度3%、酶/底物5%,水解度为23.57%;进一步利用酶解得到的大豆分离蛋白肽(SPIP)溶液与亚硒酸钠反应,通过响应面实验优化出具有高硒含量(硒含量为46.143mg/g)的大豆分离蛋白硒化多肽复合物(SPIP-Se),制备工艺条件为反应pH10、时间2h、温度78℃;并使用吸收光谱和荧光光谱对所制备的大豆分离蛋白肽-硒复合物进行结构表征,结果表明,此多肽复合物是一种不同于大豆分离蛋白肽的新型复合物;考察复合体羟基自由基清除率、还原力、金属螯合力、脂质过氧化抑制活性,结果表明,多肽复合物具有高于大豆分离蛋白肽的抗氧化活性。
Soybean protein isolate peptides(SPIP) were prepared by the biological enzymolysis technology,and the enzymolysis technology was optimized by response surface methodology using selenium binding capacity as an indicator.Results showed that optimized enzymatic hydrolysis condition was:temperature 50℃,enzyme-substrate ratio 5%(w/w),substrate concentration 3%,with a hydrolysis degree of 23.57%.To optimize the chelation technology of soybean protein isolate peptide-Se complex(SPIP-Se),the response surface method was used.Results showed that optimized chelation technology condition was;reaction time 2h,reaction temperature 78℃,pH10,the maximum selenium binding capacity of SPIP was 46.143mg/g.And the structure properties of SPIP-Se were characterized by absorption spectrum and fluorescence spectrum.The results indicated that the principal sites of selenium-binding corresponded to the carboxyl groups and carbonyl groups of SPIP.In addition,the research on hydroxyl radical scavenging activity,reducing power,metal chelating capacity and lipid peroxidation inhibition acticity showed that SPIP-Se had antioxidative effects in vitro to different degrees.
Soybean protein isolate peptides(SPIP) were prepared by the biological enzymolysis technology,and the enzymolysis technology was optimized by response surface methodology using selenium binding capacity as an indicator.Results showed that optimized enzymatic hydrolysis condition was:temperature 50℃,enzyme-substrate ratio 5%(w/w),substrate concentration 3%,with a hydrolysis degree of 23.57%.To optimize the chelation technology of soybean protein isolate peptide-Se complex(SPIP-Se),the response surface method was used.Results showed that optimized chelation technology condition was;reaction time 2h,reaction temperature 78℃,pH10,the maximum selenium binding capacity of SPIP was 46.143mg/g.And the structure properties of SPIP-Se were characterized by absorption spectrum and fluorescence spectrum.The results indicated that the principal sites of selenium-binding corresponded to the carboxyl groups and carbonyl groups of SPIP.In addition,the research on hydroxyl radical scavenging activity,reducing power,metal chelating capacity and lipid peroxidation inhibition acticity showed that SPIP-Se had antioxidative effects in vitro to different degrees.
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