牛初乳中功能性寡糖的分离纯化技术研究
详细信息 查看官网全文
- 英文论文题名：Technology forseparationand purification of functional oligosaccharides in bovine colostrum
- 论文作者：李明 ; 周家春 ; 刘振民 ; 秦臻 ; 陈启明 ; 赵黎明
- 英文论文作者：Li Ming ; Zhou Jiachun ; Liu Zhenmin ; Qin Zhen ; Chen Qiming ; Zhao Liming ; East China University of Scienceand Technology ; State Key Laboratory of Bioreactor Engineering ; Center of Separationand Extraction Technology in Fermentation Industry ; State Key Laboratory of Dairy Biotechnology ; Bright Dairy &Food Co ; Ltd
- 年：2016
- 作者机构：华东理工大学生物工程学院生物反应器工程国家重点实验室发酵工业分离提取技术研发中心;光明乳业股份有限公司技术中心乳业生物技术国家重点实验室;
- 论文关键词：牛初乳 ; 功能性寡糖 ; 分离纯化 ; 唾液酸化寡糖
- 英文论文关键词：bovine colostrum ; function aloligo saccharides ; separation and separation ; sialy-oligosacharides
- 会议召开时间：2016-11-09
- 会议录名称：中国食品科学技术学会第十三届年会论文摘要集
- 英文会议录名称：Abstracts of the 13th Annual Meeting of CIFST
- 语种：中文
- 分类号：TS252.4
- 学会代码：ZGSP
- 会议名称：中国食品科学技术学会第十三届年会
- 会议地点：中国北京
- 主办单位：中国食品科学技术学会
- 学会名称：中国食品科学技术学会
- 页数：2
- 文件大小：109k
- 原文格式：O
- 会议级别：全国
摘要
牛初乳中含有多种具有重要生理活性的寡糖,但是由于对牛初乳中寡糖的研究较少且缺乏有效分离提取方法,导致牛初乳资源极大浪费。本研究是以光明牧场牛初乳为原料,采用膜技术对牛初乳中的功能性寡糖进行初步分离,构建出一种新型树脂介质进一步分离纯化,并利用液质联用方法对纯化得到的寡糖进行结构鉴定。结果表明:10kDa超滤膜可以去除98.9%的蛋白质且总糖的回收率为96.7%;利用500Da纳滤膜去除了部分乳糖及单糖,最后得到的寡糖混合物中功能性寡糖占总合量的44%;通过液质联用鉴定出10种寡糖,其中唾液酸乳糖含量最高为1.7g/l,是牛初乳中除了乳糖外含量最高的一种功能性寡糖;构建的新型树脂对牛初乳中的唾液酸化寡糖具有较好的分离效果,同时由于其制备过程简单、成本低廉,具有大规模化分离纯化牛初乳中唾液酸化寡糖的潜力。
Bovine colostrum contains many functional oligosaccharides,but the utilization of them was low because of less research and no effective method to separation and purification.This study using membrane technology in preliminary separation,then developed a new Chromatographic packing for its further purification,use Liquid Chromatography-Mass Spectrometry to qualitative analysis of the oligosaccharides.The results show that thelOkDa ultrafiltration membrane were remove 98.9%of protein and obtained 96.7%of total sugar;500Da nanofiltration membrane to separate lactose and monosaccharide,finally obtained 44%of functional Oligosaccharides in the mixed oligosaccharide,10 oligosaccharides in the isolate was identified by LCMS and in it sialyllactose was about 1.7g/l,which was the most abundant oligosaccharides in bovine colostrum except lactose.The new material 1 have high separation efficiency for sialy-oligosacharides,due to its low cost,easer to produce it has potential to separation and purification sialy-oligosacharides in large-scale.
Bovine colostrum contains many functional oligosaccharides,but the utilization of them was low because of less research and no effective method to separation and purification.This study using membrane technology in preliminary separation,then developed a new Chromatographic packing for its further purification,use Liquid Chromatography-Mass Spectrometry to qualitative analysis of the oligosaccharides.The results show that thelOkDa ultrafiltration membrane were remove 98.9%of protein and obtained 96.7%of total sugar;500Da nanofiltration membrane to separate lactose and monosaccharide,finally obtained 44%of functional Oligosaccharides in the mixed oligosaccharide,10 oligosaccharides in the isolate was identified by LCMS and in it sialyllactose was about 1.7g/l,which was the most abundant oligosaccharides in bovine colostrum except lactose.The new material 1 have high separation efficiency for sialy-oligosacharides,due to its low cost,easer to produce it has potential to separation and purification sialy-oligosacharides in large-scale.
引文