功能化材料在磷酸化肽和糖基化肽富集中的应用
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- 英文论文题名：Application of functional nanomaterials in the enrichment of phosphopeptide/glycopeptide enrichment
- 论文作者：白玉 ; 徐林楠 ; 马雯 ; 刘虎威
- 英文论文作者：Yu Bai ; Linnan Xu ; Wen Ma ; Huwei Liu ; College of Chemistry and Molecular Engineering ; Peking University
- 年：2016
- 作者机构：北京大学化学与分子工程学院;
- 论文关键词：功能化石墨烯 ; 单分散纳米硅球 ; 酰肼基 ; 麦芽糖 ; 磷酸化肽 ; 糖基化肽
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第二十三分会：复杂样品分离分析
- 语种：中文
- 分类号：TB34;O629.7
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第二十三分会 ; 复杂样品分离分析
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：143k
- 原文格式：D
- 会议级别：全国
摘要
糖基化和磷酸化是两种非常重要蛋白质翻译后修饰,参与了诸多的生物学过程,例如糖基化参与细胞识别,蛋白质折叠和免疫响应等,而蛋白质的可逆磷酸化则调控着信号传导、基因表达、细胞周期等诸多过程,蛋白质的翻译后修饰也受到越来越多的关注。然而,由于生物样品中磷酸化蛋白的低天然丰度及复杂基质的干扰,磷酸化蛋白的质谱检测面临很大困难,而糖蛋白中的糖链具有微观不均一性和大量非糖基化肽段的干扰,其检测一直是难题。都面临着分析前对糖基化/磷酸化蛋白/肽段进行选择性富集地问题。我们的研究中合成了胍基功能化石墨烯,酰肼基功能化单分散的纳米硅球,并成功用于磷酸化多肽的特异性富集,酰肼基功能化单分散的纳米硅球可以对不同磷酸化位点的磷酸化多肽进行区分[1,2];此外,合成了麦芽糖功能化的金属有机骨架(MOFs)材料,成功用于用于糖基化多肽的高灵敏度高选择性富集。
Glycosylation and phosphorylation are the most significant post translational modification that are involved in varios biological process.The direct analysis of phosphorylated proteins/peptides by mass spectrometry(MS) can hardly be realized because of their low stoichiometry,poor ionization efficiency and signal suppression by non-phosphorylated species with high abundance.On the other side,the low abundance of glycopeptides together with the heterogeneity of glycosylation sites and severe signal suppression by high abundant non-glycopeptides make it difficult to analyze glycosylation byMS.Therefore,the enrichment of phosphopeptides/glycopeptide prior to MS analysis are demanded for their effective detection.In our research,guanidyl-functionalized graphene(GFG) and hydrazide functionalized monodispersed silica microsphere(HFMSM) were developed to enrich the phosphopeptides,and the maltose-functionalized MIL-101(Cr) was synthesized for glycopeptide enrichment.
Glycosylation and phosphorylation are the most significant post translational modification that are involved in varios biological process.The direct analysis of phosphorylated proteins/peptides by mass spectrometry(MS) can hardly be realized because of their low stoichiometry,poor ionization efficiency and signal suppression by non-phosphorylated species with high abundance.On the other side,the low abundance of glycopeptides together with the heterogeneity of glycosylation sites and severe signal suppression by high abundant non-glycopeptides make it difficult to analyze glycosylation byMS.Therefore,the enrichment of phosphopeptides/glycopeptide prior to MS analysis are demanded for their effective detection.In our research,guanidyl-functionalized graphene(GFG) and hydrazide functionalized monodispersed silica microsphere(HFMSM) were developed to enrich the phosphopeptides,and the maltose-functionalized MIL-101(Cr) was synthesized for glycopeptide enrichment.
引文
[1]Xu L.N.,Li L.P.,Jin L.,Bai Y.and Liu H.W.Chem.Commun.,2014,50:10963.
[2]Xu L.N.,Ma W.,Shen S.S.,Li L.P.,Bai Y.and Liu H.W.,Chem.Commun.,2016,52:1162.
[2]Xu L.N.,Ma W.,Shen S.S.,Li L.P.,Bai Y.and Liu H.W.,Chem.Commun.,2016,52:1162.