CO_2响应的蜂窝状多孔膜及其在细胞培养中的应用
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- 英文论文题名：CO_2-induced smart honeycomb film for cell adhesion
- 论文作者：殷鸿尧 ; 冯玉军
- 英文论文作者：Hongyao Yin ; Yujun Feng ; Polymer Research Institute ; Sichuan University
- 年：2016
- 作者机构：四川大学高分子研究所高分子材料工程国家重点实验室;
- 论文关键词：两亲嵌段共聚物 ; 有序多孔膜 ; 表面润湿性
- 会议召开时间：2016-07-01
- 会议录名称：中国化学会第30届学术年会摘要集-第三十一分会：胶体与界面化学
- 语种：中文
- 分类号：TQ317
- 学会代码：ZGHY
- 会议名称：中国化学会第30届学术年会-第三十一分会 ; 胶体与界面化学
- 会议地点：中国辽宁大连
- 主办单位：中国化学会
- 学会名称：中国化学会
- 页数：1
- 文件大小：158k
- 原文格式：D
- 会议级别：全国
摘要
蜂窝状有序多孔膜因其表面具有高度规整的微米或纳米级的孔结构,所以在纳米颗粒自组装、疏水材料、组织工程等领域具有重要的应用前景。作为一种界面材料,其表面性能在实际应用中至关重要。近年来,构建表面润湿性具有刺激响应性能的多孔膜已经成为该领域的研究热点。本文利用原子转移自由基聚合方法成功制备了系列两嵌段聚合物PS-b-PDMAEMA,再通过"呼吸图案法"(Breath Figure)制备出高度规整的有序多孔膜。研究发现,基于PS-b-PDMAEMA的多孔膜由于聚合物本身的疏水性及表面微孔的存在而表现出较强的疏水性能。利用CO_2质子化含叔胺结构的PDMAEMA后,多孔膜由疏水性转变为亲水性。进一步研究发现,将该系列多孔膜用作HaCaT细胞培养的基底,5%CO_2氛围能够直接改善多孔膜的表面润湿性,因而导致HaCaT细胞更容易附着在多孔膜表面。
Honeycomb-patterned porous films with reversible stimuli-responsive surface wettability between hydrophobicity and hydrophilicity have versatile potentials in many applications,including controlled drug delivery,cells culture and release,catalysis,and oil/water separation.In this work,highly ordered porous films were prepared from a series of diblock copolymers polystyrene-b-poly(N,N-diethylamino ethyl methacrylate)(PS-b-PDMAEMA) using Breath Figure technique.It was demonstrated that molecular hydrophilicity plays an essential role in determining film structure,including pore size,porous layers and regularity.Moreover,reversible and tunable surface wettability of these porous films has been achieved simply by alternate introduction and removal of CO_2.For the first time,the original hydrophobic porous surfaces are directly used as scaffolds for cell culture with 5%CO_2atmosphere to enhance the interaction with cells during cell culture process,thus resulting in better cell attachment and proliferation,indicative of these smart honeycomb films shows advantages over normal honeycomb films.
Honeycomb-patterned porous films with reversible stimuli-responsive surface wettability between hydrophobicity and hydrophilicity have versatile potentials in many applications,including controlled drug delivery,cells culture and release,catalysis,and oil/water separation.In this work,highly ordered porous films were prepared from a series of diblock copolymers polystyrene-b-poly(N,N-diethylamino ethyl methacrylate)(PS-b-PDMAEMA) using Breath Figure technique.It was demonstrated that molecular hydrophilicity plays an essential role in determining film structure,including pore size,porous layers and regularity.Moreover,reversible and tunable surface wettability of these porous films has been achieved simply by alternate introduction and removal of CO_2.For the first time,the original hydrophobic porous surfaces are directly used as scaffolds for cell culture with 5%CO_2atmosphere to enhance the interaction with cells during cell culture process,thus resulting in better cell attachment and proliferation,indicative of these smart honeycomb films shows advantages over normal honeycomb films.
引文
[1]Bunz,U.H.F.Adv.Mater.2006,18:973.
[2]Escalé,P.;Rubatat,L.;Billon,L.;Save,M.Eur.Polym.J.2012,48:1001.
[3]Yin,H.;Bulteau,A.L.;Feng,Y.;Billon,L.Adv.Mater.Interfaces 2016,10.1002/admi.201500623.
[2]Escalé,P.;Rubatat,L.;Billon,L.;Save,M.Eur.Polym.J.2012,48:1001.
[3]Yin,H.;Bulteau,A.L.;Feng,Y.;Billon,L.Adv.Mater.Interfaces 2016,10.1002/admi.201500623.