渤海湾潮间带柱状沉积物中微生物群落结构
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摘要
采用变性凝胶梯度电泳(PCR-DGGE)技术初步调查了渤海湾子牙河和南排河入海口潮间带0-100cm柱状沉积物中微生物群落优势种分布特征。结果表明子牙河入海口柱状沉积物微生物群落多样性在2.85~3.35之间,南排河入海口柱状沉积物微生物群落多样性在2.97~3.22之间;两个潮间带沉积物中微生物群落随深度的加深,其优势种组成及其丰度、多样性产生一定的波动,但没有明显的规律性。
引文
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[2]SHI W,BECKER J,BISCHOFF M et al.Association of microbial community composition and activity with lead,chromium,and hydrocarbon contamination.Appl Environ Microbiol,2002,68(8):3859-3866.
[3]GORVAM MS,BULLIMORE J,BALL AS,et al.Responses of active bacterial and fungal communities in soils under winter wheat to different fertilizer and pesticide regimens.2004,Appl Environ Microbiol,70(5):2692-2701.
[4]ZOU X,WANG Y,WANG X et al.Environmental stress and policy guidelines for environmental management of sanya coastal zone,Hainan Island,China.J Coastal Res,2004,43(Special Issue):244-253.
[5]BONDI G,MIRZA UB,Al-OMAIR A et al.Spatial distribution of Polychlorinated Biphenyls in coastal marine sediments receiving industrial effluents in Kuwait Arch Environ Con Tox,2006,50;166-174.
[6]Chen MR,Yu LZ,Xu SY.Spatial distribution of Polychlorinated Biphenyls(PCBs)contaminants in the tidal-flat sediments of Yangtze Estuary.Mar Sci Bull,2003,5(2):22-28.
[7]MENG W,LEI K,ZHENG B,et al.Modern sedimentation rates in the intertidal zone on the west coast of the Bohai Gulf.Acta Oceanol Sin,2005,24(3):46-53.
[8]雷坤,孟伟,郑丙辉,等.渤海湾西岸潮间带沉积物粒度分布特征.海洋通报,2006,25(1):54-61.
[9]LI H,FU YZ,ZHOU CG et al.Distribution characteristics of organochlorine Pestieides and PCBs in the surface sediments in Dalian Bay and Jinzhou Bay.Mar Environ Sci,1998,17:73-76.
[10]MA M,FENG Z,GUAN C et al.DDT,PCBs and PAHs in sediment from the intertidal zones of the Bohai Sea and the Yellow Sea.Mar Poll Bull,2001,42(2):132-136.
[11]ZHANG P,SONGJM,YUAN HM.Persistent organic pollutants(POPs)residue in the sediments and mollusks of the Bohai Sea coastal areas,North China.Environ Int,2009,35:632-646.
[12]GUO YQ,ZHANG ZN,MU FH.Biomass of meiobenthic in the Bohai Sea,China.Acta Oceanol Sin,2001,20(3):435-442.
[13]王瑜,刘录三,刘存歧,等.渤海湾近岸海域春季大型底栖动物群落特征.环境科学研究,2010,23(4):430-436.
[14]SHELFFIELD VC,COX DR,MYERS RM.Attachment of a 40bp G+C rich sequence(GC clap)to genomic DNA fragments by polymerase chain reaction results in improved detection of single-base changes.Proc Natl Acad Sci USA,1989,86:232-236.
[15]GLORIA S,SARA S,LUCIA A,et al.Investigation of the microbial ecology of Ciauscolo,a traditional Italian salami,by culture-dependent techniques and PCR-DGGE.Meat Science,2007,77:413-423.
[16]PAYNE MS,HALL MR,BANNISTER R,et al.Microbial diversity within the water column of a larval rearing system for the ornate rock lobster(Panulirus omatus).Aquaculture,2006,258;80-90.
[17]YING YL,LV ZM,MIN H,et al.Dynamic changes of microbial community diversity in photohydrogen producing reactor monitored by PCR-DGGE.J Environ Sci,2008,20:1118-1125.
[18]罗海峰,齐鸿雁,薛凯,等.PCR-DGGE技术在农田土壤微生物多样性研究中的应用.生态学报,2003,23(8):1570-1575.
[19]赵兴青,杨柳燕,陈灿,等.PCR-DGGE技术用于湖泊沉积物种微生物群落结构多样性研究.生态学报,2006,26(11):3610-3616.
[20]BRAMBILLA E,HIPPE H,HAGELSTEIN A,et al.2001.16S rDNA diversity of cultured and uncultured prokaryotes of a mat sample from Lake Fryxell,McMurdo Dry Valleys,Antarctica.Extremophiles,5(1):23-33.
[21]TORSVIK V,DAAE FL,SANDAA RA,et al.Novel techniques for analyzing microbial diversity in natural and perturbed environments.J Biotechnol,1998,64(1):53-62.
[22]CLEMENT BG,KEHL LE,DEBORD KL,et al.Terminal restriction fragment patterns(TRFPs),a rapid,PCR-based method for the comparison of complex bacterial communities.J Microbiol Meth,1998,31:135-142.
[23]RANJARD L,POLY F,NAZARET S.Monitoring complex bacterial communities using culture-independent molecular techniques:application to soil environment Res Microbiol,2000,151:167-177.
[24]DIEZ B,PEDROS-ALIO C,MARSH TL,et al.Application of denaturing gradient gel electrophoresis(DGGE)to study the diversity of marine picoeukaryotic assemblages and comparison of DGGE with other molecular techniques.Appl Microbiol Biotech,2001,67:2943-2951.
[25]ROOMEY-VARGA JN,ANDERSON RT,FRAGA JL,et al.Microbial cummunities associated with anaerobic benzen degradation in a petroleum-contaminated aquifer.Appl Environ Microbiol,1999,56:3056-3063.
[26]SCHAUER M,MASSANA R,PEDROS-ALIO C,Spatial differences in bacterioplankton composition along the Catalan coast(NW Mediterranean)assessed by molecular fingerprinting.FEMS Microbiol Ecol,2000,33:51-59.
[27]ATLAS RM.Diversity of microbial communities.Adv Microb Ecol,1984,7:1-47.
[28]BOON N,MARLE C,TOP EM,et al.Comparison of the spatial homogeneity of physicochemical parameters and bacterial 16S rRNA genes in sediment samples from a dumping site for dredging sludge.Appl Microbiol Biotech,2000,53:742-747.
[29]秦延文,孟伟,郑丙辉,等.渤海湾天津段潮间带沉积物柱状样重金属污染特征.环境科学,2006,27(2):268-273.