13种民间中草药体外抗肿瘤活性筛选研究
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- 英文论文题名：Screening of 13 kinds of Folk Chinese herbal medicine in vitro antitumor activity
- 论文作者：司金光 ; 张涛 ; 王孟华 ; 邹忠梅
- 英文论文作者：SI Jin-guang ; ZHANG Tao ; WANG Meng-hua ; ZOU Zhong-mei ; Henan University of Traditional Chinese Medicine ; Institute of Medicinal Plant Development ; Chinese Academy of Medical Sciences and Peking Union Medical College
- 年：2016
- 作者机构：河南中医药大学;中国医学科学院北京协和医学院药用植物研究所;
- 论文关键词：云南 ; 民间中草药 ; 提取物 ; 抗肿瘤 ; MTT
- 英文论文关键词：Yunnan Province ; Folk Chinese herbal medicine ; extracts ; anti-tumor ; MTT
- 会议召开时间：2016-09-27
- 会议录名称：中国药学会第十三届青年药学科研成果交流会论文集
- 语种：中文
- 分类号：R285
- 学会代码：YYWS
- 会议名称：中国药学会第十三届青年药学科研成果交流会
- 会议地点：中国江西南昌
- 主办单位：中国药学会
- 学会名称：中国药学会
- 页数：6
- 文件大小：885k
- 原文格式：O
- 会议级别：全国
摘要
目的:研究采自云南的12种(刺金刚、扁担藤、白川牛胆、羊屎果、艾堇、蜡烛果、古钩藤、下果藤、老虎芋、灯笼草、两面青、盒果藤)和1种采自海南的维沙杜共计13种民间中草药的提取物在抗肿瘤方面的应用价值。方法:采用MTT法,测定各中草药提取物对人肝癌(HepG2)细胞、人子宫宫颈癌(Hela)、人胃癌(MGC-803)细胞和人肺癌(A549)细胞等四株癌细胞系的增殖抑制作用。结果:蜡烛果和两面青不同浓度乙醇提取物对四株癌细胞系均具有显著的增殖抑制作用。其中蜡烛果50%乙醇提取物对四株癌细胞系的IC_(50)值分别为:44.66±8.69μg/mlCHepG2)、19.32±5.23 ug/ml(Hela)、16.27±3.16μg/ml(MGC-803)、49.32±1.17μg/ml(A549);蜡烛果95%乙醇提取物对四株癌细胞系的IC_(50)值分别为:53.17±5.99μg/ml(HepG2)、25.89±5.87μg/m1(Hela)、20.75±2,82μg/ml(MGC-803)、45.64±1.27μg/ml(A549);两面青50%乙醇提取物对四株癌细胞系的IC_(50)值分别为:11.50±2.70μg/ml(HepG2)、7.90±1.48μg/ml(Hela)、12.48±2.74μg/ml(MGC-803)、23.46±5.86μg/ml(A549)。结论:蜡烛果和两面青具有较好的体外抗肿瘤活性,为其进一步的开发提供科学依据。
Objective To evaluate the extracts of 13 kinds of Chinese herbal medicine used in folk,12 of these plants come from Yunnan Province.China,including Smilax china,Tetrastigma planicaule,Sheepear Inula,Syzygium cunini,Sauropus bacciformis,Aegiceras corniculatum,Cryptolepis buchananii,Gouania leptostachya,Alocasia cucullata,Clinopodium polycephalum,Maesa indica,Operculina turpethum and 1 come from Hainan Province.China named Wissadula periplocifolia,for their properties on anti-tumor.METHODS The inhibition effect of tumor cells,such as HepG2,Hela,MGC-803 and A549,were tested by MTT method in vitro.RESULTS Ethanol extract of different concentration of Aegiceras comiculatum and Maesa indica had a significant inhibition effect on the four tumor cell lines.The IC_(50)values of the extract of 50%ethanol of Aegiceras corniculatum were 44.66±8.69 μg/ml(HepG2)、19.32±5.23 μg/ml(Hela)、16.27±3.16 μg/ml(MGC-803)and 49.32±1.17 μg/ml(A549),respectively.The IC_(50)values of the extract of 95%ethanol of Aegiceras comiculatum were 53.17±5.99 μg/ml(HepG2)、25.89±5.87 μg/ml(Hela)、20.75±2,82 μg/ml(MGC-803)and 45.64±1.27μg/ml(A549),respectively.The IC_(50)values of the extract of 50%ethanol of Maesa indica were 11.50±2.70 μg/ml(HepG2)、7.90±1.48 μg/ml(Hela)、12.48±2.74 μg/ml(MGC-803)and 23.46±5.86 μg/ml(A549),respectively.CONCLUSION Aegiceras corniculatum and Maesa indica had a positive activity on anti-tumor in vitro,and it provide scientific basis for its further development.
Objective To evaluate the extracts of 13 kinds of Chinese herbal medicine used in folk,12 of these plants come from Yunnan Province.China,including Smilax china,Tetrastigma planicaule,Sheepear Inula,Syzygium cunini,Sauropus bacciformis,Aegiceras corniculatum,Cryptolepis buchananii,Gouania leptostachya,Alocasia cucullata,Clinopodium polycephalum,Maesa indica,Operculina turpethum and 1 come from Hainan Province.China named Wissadula periplocifolia,for their properties on anti-tumor.METHODS The inhibition effect of tumor cells,such as HepG2,Hela,MGC-803 and A549,were tested by MTT method in vitro.RESULTS Ethanol extract of different concentration of Aegiceras comiculatum and Maesa indica had a significant inhibition effect on the four tumor cell lines.The IC_(50)values of the extract of 50%ethanol of Aegiceras corniculatum were 44.66±8.69 μg/ml(HepG2)、19.32±5.23 μg/ml(Hela)、16.27±3.16 μg/ml(MGC-803)and 49.32±1.17 μg/ml(A549),respectively.The IC_(50)values of the extract of 95%ethanol of Aegiceras comiculatum were 53.17±5.99 μg/ml(HepG2)、25.89±5.87 μg/ml(Hela)、20.75±2,82 μg/ml(MGC-803)and 45.64±1.27μg/ml(A549),respectively.The IC_(50)values of the extract of 50%ethanol of Maesa indica were 11.50±2.70 μg/ml(HepG2)、7.90±1.48 μg/ml(Hela)、12.48±2.74 μg/ml(MGC-803)and 23.46±5.86 μg/ml(A549),respectively.CONCLUSION Aegiceras corniculatum and Maesa indica had a positive activity on anti-tumor in vitro,and it provide scientific basis for its further development.
引文
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[5].高嘉屿,杨櫹,尹卫平.11种伏牛山野生抗癌药用植物活性筛选及研究[J].辽宁中医杂志,2015,42(12):2393-2396
[2].代敏,李霓,李倩,等.中国肿瘤预防控制概况[J].中国肿瘤,2011,20(12):868-873.
[3].顾琳娜,顾吴.天然抗肿瘤药物筛选方法的研究进展[J].医药导报,2009,28(4):496-498.
[4].杨柳萌,郑昌博,普岳红,等.云南省药物研究的优势_现状和问题[J].云南科技管理,201 1,3:7-10.
[5].高嘉屿,杨櫹,尹卫平.11种伏牛山野生抗癌药用植物活性筛选及研究[J].辽宁中医杂志,2015,42(12):2393-2396