FT-IR技术快速鉴定硫酸盐还原菌
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- 英文论文题名：Identification and Characterization of Sulfate Reducing Bacteria by FT-IR Spectroscopy Method
- 论文作者：郑碧娟 ; 刘娜 ; 罗婷 ; 吴亚楠 ; 刘宏伟 ; 刘宏芳
- 英文论文作者：ZHENG Bi-juan ; LIU Na ; LUO Ting ; WU Ya-nan ; LIU Hong-wei ; LIU Hong-fang ; School of Chemistry and Chemical Engineering ; Hubei Key Laboratory of Materials Chemistry and Service Failure ; Huazhong University of Science and Technology ; CCCC Wuhan Harbour Engineering Design and Research Co. ; Ltd.
- 年：2014
- 作者机构：华中科技大学化学与化工学院材料化学与服役失效湖北省重点实验室;中交武汉港湾工程设计研究院有限公司;
- 论文关键词：傅里叶变换红外光谱 ; 硫酸盐还原菌 ; 聚合酶链反应
- 英文论文关键词：fourier transform infrared spectroscopy ; sulfate reducingbacteria ; polymerase chain reaction
- 会议召开时间：2014-07-28
- 会议录名称：第十八届全国缓蚀剂学术讨论会论文集
- 语种：中文
- 分类号：Q93-331;TE980
- 学会代码：ZGHA
- 会议名称：第十八届全国缓蚀剂学术讨论及应用技术经验交流会
- 会议地点：中国四川成都
- 主办单位：中国腐蚀与防护学会缓蚀剂专业委员会
- 学会名称：中国腐蚀与防护学会缓蚀剂专业委员会
- 页数：4
- 文件大小：1434k
- 原文格式：O
- 会议级别：全国
摘要
本文采用傅里叶变换红外光谱(FT-IR)法,研究了湛江(ZJ)和渤海(BH)油田污水中硫酸盐还原菌(SRB)的特征峰,并结合两株细菌的生理生化特性进行了菌种鉴定,研究结果表明结合标准菌株的FT-IR特征峰位置的吸光度,FT-IR法可快速分析并判断ZJ油田SRB属于脱硫脱硫弧菌属,BH油田SRB为脱硫肠状菌属。同时运用分子生物学方法聚合酶链反应(PCR)对SRB的16S rDNA基因特征性序列进行测序,鉴定结果与FT-IR的结果吻合。因此FT-IR可作为一种快速鉴定SRB类群的方法。
Fourier transform infrared spectroscopy(FT-IR) was applied to study the specific peaks of sulfate reducing bacteria(SRB) of Zhanjiang(ZJ) and Bohai(BH) oil field wastewater,combined with the physiological and biochemical features of these bacteria species,the SRB of ZJ oil was primarily determined as Desulfovibrio and the SRB of BH oil as Desulfotomaculum.On the other hand,molecular biological method of polymerase chain reaction(PCR) was utilized to characterize SRB by the feature sequences of 16 S rDNA gene,the results by PCR coincided with the results by FT-IR.In conclusion,FT-IR method can be used to rapidly and accurately characterize and identify SRB.
Fourier transform infrared spectroscopy(FT-IR) was applied to study the specific peaks of sulfate reducing bacteria(SRB) of Zhanjiang(ZJ) and Bohai(BH) oil field wastewater,combined with the physiological and biochemical features of these bacteria species,the SRB of ZJ oil was primarily determined as Desulfovibrio and the SRB of BH oil as Desulfotomaculum.On the other hand,molecular biological method of polymerase chain reaction(PCR) was utilized to characterize SRB by the feature sequences of 16 S rDNA gene,the results by PCR coincided with the results by FT-IR.In conclusion,FT-IR method can be used to rapidly and accurately characterize and identify SRB.
引文
[1]刘宏芳,汪梅芳,许立铭.硫酸盐还原菌腐蚀的微生物防治研究进展[J].腐蚀科学与防护技术,2003,15(3):161-163.
[2]马放,魏利.油田硫酸盐还原菌分子生态学及其活性生态调控研究[M].北京:科学出版社,2009.
[3]刘宏芳,许立铭,郑家燊.硫酸盐还原菌生物膜下钢铁腐蚀研究概况[刀.油田化学.2000,17(1):93-95.
[4]王明义,梁小兵,郑娅萍,等.硫酸盐还原菌鉴定和检测方法的研究进展[J].微生物学杂志,2005,25(6):81-84.
[5]KRISTIAN DALY,RiCHARB J SHARP,AlAN J MCCARTHY.Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria[J].Microbiology,2000,146:1693-1705.
[6]刘宏芳,黄茁,许立铭,等.气相色谱质谱在不同种类硫酸盐还原菌的鉴定中应用探讨[J].分析化学,2000,28(2):190-193.
[7]NGO-THI N A,KIRSCHNER C,NAUMANN D.Characterization and identi?cation of microorganisms by FT-IR microspectrometry[J].Journal of Molecular Structure,2003:371-380.
[8]CelBStE RUBIO,ChrlSTEIIE Ott,CarOIINE AMIEL Sulfato/thiosulfato reducing bacteria characterization by FT-IR spectroscopy:A new approach to biocorrosion control[J].Microbiological Methods,2006,26:287-296.
[9]布坎南R E,吉本斯编N E.伯杰细菌鉴定手册[M].中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组译,(第八版)1984.
[10]穆军,韦华生,焦炳华.一株抗菌海洋硫酸盐还原菌的筛选和鉴定[J].大连铁道学院学报,2006,27(3):89-92.
[11]刘宏芳,刘涛.嗜热硫酸盐还原菌生长特征及其对碳钢腐蚀的影响[J].中国腐蚀与防护报,2009,29(2):93-98.
[12]DONG Z H,LIU T,LIU H F.Influence of EPS isolated from thermophilic sulphate-reducing bacteria on carbon steel corrosion[J].Biofouling:The Journal of Bioadhesion and Biofilm Research,2011,27(5):487-495
[13]陈悟,余龙江,李春艳,等.从油田分离的两株嗜热产孢子硫酸盐还原茵系统进化分析[J].应用与环境生物学报,2005,11(6):733-737.
[14]PIERRE-POI LIEBGTT,MANON JOSEPH,MARIE-LAURE FARDEAU.Clostridiisalibacter paucivorans gen.nov.,sp.nov.,a novel moderately halophilic bacterium isolated from olive mill wastewater[J].International Journal of Systematic and Evolutionary Microbiology.2008,58:61-67.
[2]马放,魏利.油田硫酸盐还原菌分子生态学及其活性生态调控研究[M].北京:科学出版社,2009.
[3]刘宏芳,许立铭,郑家燊.硫酸盐还原菌生物膜下钢铁腐蚀研究概况[刀.油田化学.2000,17(1):93-95.
[4]王明义,梁小兵,郑娅萍,等.硫酸盐还原菌鉴定和检测方法的研究进展[J].微生物学杂志,2005,25(6):81-84.
[5]KRISTIAN DALY,RiCHARB J SHARP,AlAN J MCCARTHY.Development of oligonucleotide probes and PCR primers for detecting phylogenetic subgroups of sulfate-reducing bacteria[J].Microbiology,2000,146:1693-1705.
[6]刘宏芳,黄茁,许立铭,等.气相色谱质谱在不同种类硫酸盐还原菌的鉴定中应用探讨[J].分析化学,2000,28(2):190-193.
[7]NGO-THI N A,KIRSCHNER C,NAUMANN D.Characterization and identi?cation of microorganisms by FT-IR microspectrometry[J].Journal of Molecular Structure,2003:371-380.
[8]CelBStE RUBIO,ChrlSTEIIE Ott,CarOIINE AMIEL Sulfato/thiosulfato reducing bacteria characterization by FT-IR spectroscopy:A new approach to biocorrosion control[J].Microbiological Methods,2006,26:287-296.
[9]布坎南R E,吉本斯编N E.伯杰细菌鉴定手册[M].中国科学院微生物研究所《伯杰细菌鉴定手册》翻译组译,(第八版)1984.
[10]穆军,韦华生,焦炳华.一株抗菌海洋硫酸盐还原菌的筛选和鉴定[J].大连铁道学院学报,2006,27(3):89-92.
[11]刘宏芳,刘涛.嗜热硫酸盐还原菌生长特征及其对碳钢腐蚀的影响[J].中国腐蚀与防护报,2009,29(2):93-98.
[12]DONG Z H,LIU T,LIU H F.Influence of EPS isolated from thermophilic sulphate-reducing bacteria on carbon steel corrosion[J].Biofouling:The Journal of Bioadhesion and Biofilm Research,2011,27(5):487-495
[13]陈悟,余龙江,李春艳,等.从油田分离的两株嗜热产孢子硫酸盐还原茵系统进化分析[J].应用与环境生物学报,2005,11(6):733-737.
[14]PIERRE-POI LIEBGTT,MANON JOSEPH,MARIE-LAURE FARDEAU.Clostridiisalibacter paucivorans gen.nov.,sp.nov.,a novel moderately halophilic bacterium isolated from olive mill wastewater[J].International Journal of Systematic and Evolutionary Microbiology.2008,58:61-67.