H9N2 AIV对肉鸡肠道菌群和肠道组织紧密连接的影响
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- 英文论文题名：The Effect of Intestinal Flora and Intestinal Tight Junctions in Broilers Infected with H9N2 Subtype Influenza Virus
- 论文作者：刘晓琳 ; 吴彻 ; 陈彤 ; 李鸿鑫 ; 严一铭 ; 谢青梅
- 英文论文作者：Xiaolin Liu ; Che Wu ; Tong chen ; Hongxin Li ; Yiming Yan ; Qinmei Xie ; College of Animal Science ; South China Agricultural University
- 年：2016
- 作者机构：华南农业大学动物科学学院;
- 论文关键词：H9N2AIV ; 肠道菌群 ; Illumina Hiseq 2000测序平台 ; qPCR ; 紧密连接
- 英文论文关键词：H9N2 AIV ; Intestinal flora ; Illumina Hiseq 2000 sequencing flat ; qPCR ; Tight junction
- 会议召开时间：2016-11-04
- 会议录名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会论文集
- 语种：中文
- 分类号：S858.31
- 学会代码：ZGXJ
- 会议名称：中国畜牧兽医学会动物微生态学分会第五届第十二次全国学术研讨会
- 会议地点：中国山东青岛
- 主办单位：中国畜牧兽医学会动物微生态学分会
- 学会名称：中国畜牧兽医学会
- 页数：1
- 文件大小：81k
- 原文格式：O
- 会议级别：全国
摘要
H9N2亚型禽流感病毒(H9N2AIV)是一种低致病性禽流感病毒,其临床症状表现温和,易继发细菌感染和支原体感染,对我国畜牧业造成严重的经济损失。本研究通过Illumina Hiseq 2000测序技术和实时荧光定量PCR(qPCR)的方法研究H9N2AIV感染后肉鸡肠道菌群结构及其肠道粘膜组织紧密连接的变化,为H9N2AIV继发感染的防控提供理论指导,为进一步研发抗流感病毒的微生态制剂提供理论依据。研究表明:1)感染H9N2 AIV改变了肉鸡肠道菌群组成和各菌属相对丰度,且盲肠粘膜上缺少考拉杆菌属,出现Sutterella;2)与空白组相比,攻毒组鸡在攻毒后3天、5天埃希氏菌属在回肠粘膜和盲肠粘膜上显著增加(p<0.05);攻毒后第12天埃希氏菌属在回肠粘膜和盲肠粘膜上极显著减少(p<0.01);3)攻毒后第5天,回肠绒毛长度显著降低(p<0.05);盲肠粘膜结构蛋白ZO-1和Occludin极显著下调(p<0.01)。攻毒后第12天,回肠隐窝显著降低(p<0.05),绒毛长度/隐窝深度显著减少(p<0.05),Occludin mRNA表达量显著下调(p<0.05);盲肠粘膜上Occludin mRNA表达量下调极显著(p<0.01)。本研究表明H9N2AIV感染能够造成肠道菌群紊乱,可显著影响条件致病菌数量,感染后肠道粘膜组织紧密连接降低,增加了宿主继发感染的机会。
H9N2 subtype avian influenza virus(H9N2 AIV) is a low pathogenic avian influenza virus with the mild clinical symptoms,and it causes secondary infection of bacterium or some virus easily,which causing serious economic losses to livestock in China.This study investigated changes of the structure of intestinal flora and intestinal tight junctions on broilers infected with H9N2 AIV by Illumina the Hiseq 2000 sequencing technology and Real-time Quantitative polymerase chain reaction(qPCR) technology,providing theoretical guidance for the H9N2 AIV secondary infection prevention and control,and provide a new theoretical basis for the further development of anti-influenza virus probiotics.Results show as follow:1) The composition and relative abundance of intestinal flora in the infection group were changed.The cecal mucosa flora of boilers infected with H9N2 AIV existed Sutterella but Phascolarctobacterium.2)Compared with the mock group,at 5 days post-infection,Escherichia of the infection group increased significantly(p<0.05) in the ileal mucosa,increase in cecal mucosa;at day 12 post-infection,Escherichia the infection group decreased significantly(p<0.01) in the ileal mucosa,reduceing in cecal mucosa.3) At day 5 post-infection,the ileal intestinal villus length decreased significantly(p<0.05);Cecal mucosa structural protein ZO-1 and Occludin decreased very significantly(p<0.01);at day 12 post-infection,ileal crypt reduced significantly(p<0.05),the ratro of villus length and crypt depth was significantly reduced(p<0.05),Occludin mRNA expression quantity decreased significantly(p<0.05);cecal mucosa Occludin mRNA expression quantity decreased very significantly(p<0.01).This study indicated that H9N2 AIV infected boilers could cause intestinal flora disorder,variation of conditioned pathogen and the infection caused intestinal mucosal tight junction reduced,which increase the chance of secondary infection.
H9N2 subtype avian influenza virus(H9N2 AIV) is a low pathogenic avian influenza virus with the mild clinical symptoms,and it causes secondary infection of bacterium or some virus easily,which causing serious economic losses to livestock in China.This study investigated changes of the structure of intestinal flora and intestinal tight junctions on broilers infected with H9N2 AIV by Illumina the Hiseq 2000 sequencing technology and Real-time Quantitative polymerase chain reaction(qPCR) technology,providing theoretical guidance for the H9N2 AIV secondary infection prevention and control,and provide a new theoretical basis for the further development of anti-influenza virus probiotics.Results show as follow:1) The composition and relative abundance of intestinal flora in the infection group were changed.The cecal mucosa flora of boilers infected with H9N2 AIV existed Sutterella but Phascolarctobacterium.2)Compared with the mock group,at 5 days post-infection,Escherichia of the infection group increased significantly(p<0.05) in the ileal mucosa,increase in cecal mucosa;at day 12 post-infection,Escherichia the infection group decreased significantly(p<0.01) in the ileal mucosa,reduceing in cecal mucosa.3) At day 5 post-infection,the ileal intestinal villus length decreased significantly(p<0.05);Cecal mucosa structural protein ZO-1 and Occludin decreased very significantly(p<0.01);at day 12 post-infection,ileal crypt reduced significantly(p<0.05),the ratro of villus length and crypt depth was significantly reduced(p<0.05),Occludin mRNA expression quantity decreased significantly(p<0.05);cecal mucosa Occludin mRNA expression quantity decreased very significantly(p<0.01).This study indicated that H9N2 AIV infected boilers could cause intestinal flora disorder,variation of conditioned pathogen and the infection caused intestinal mucosal tight junction reduced,which increase the chance of secondary infection.
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