基于AOP原理的皮肤致敏测试替代方法进展
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- 英文论文题名：Development of Alternative Test Strategy for the AOP-based Skin Sensitization
- 论文作者：陈彧 ; 喻欢 ; 程树军 ; 谈伟君 ; 刘超
- 英文论文作者：Yu Chen ; Huan Yu ; Shujun Cheng ; Weijun Tan ; Chao Liu ; Guangdong Inspection and Quarantine Technology Center ; Sun Yat-sen University ; Guangdong Pharmaceutical University
- 年：2016
- 作者机构：广东出入境检验检疫局技术中心;广东药学院;中山大学;
- 论文关键词：有害结局通路 ; AOP ; 皮肤致敏 ; 过敏性接触性皮炎
- 英文论文关键词：adverse outcome pathway ; AOP ; skin sensitization ; allergic contact dermatitis
- 会议召开时间：2016-06-15
- 会议录名称：第十一届中国化妆品学术研讨会论文集
- 英文会议录名称：The Proceedings of the 11th Seminar on Cosmetic China
- 语种：中文
- 分类号：R758.22
- 学会代码：ZGXE
- 会议名称：第十一届中国化妆品学术研讨会
- 会议地点：中国上海
- 主办单位：中国香料香精化妆品工业协会
- 学会名称：中国香料香精化妆品工业协会
- 页数：7
- 文件大小：563k
- 原文格式：O
- 会议级别：全国
摘要
皮肤局部接触某些化学品可引起免疫性皮肤反应,传统的皮肤致敏试验采用豚鼠进行,在3R原则和现代科技发展的推动下,多项动物试验的优化方法和替代方法被开发并通过验证认可,如LLNA、DPRA、KeratinoSens~(TM)和CLAT等。替代方法的开发适应了日益增加的化学品和化妆品的致敏筛查需求。目前,基于有害结局通路概念开发更符合皮肤生物学机制的替代方法,应用整合测试策略在考虑证据权重和兼顾成本效益的前提下,将适用范围不同的体外方法方法进行组合,是现阶段皮肤致敏替代方法研发的重要方向。
Allergic contact dermatitis,or skin sensitization,is an inflammatory reaction controlled by the immune response induced by foreign substances.Animal models such as the guinea pig maximization test were traditionally used to estimate whether a chemical has the potential to induce skin sensitization in humans.Under the promote if 3Rs principle and modernization technologies,several refined animal testing and in vitro screening methods have been developed for identifying potential contact allergens,such as the murine local lymph node assay(LLNA),DPRA,KeratinoSens? and h-CLAT.The development of in vitro methods is suitable to predict sensitization hazard from chemicals and cosmetics.More recently,the theory underlying this approach has been formalized in the "adverse outcome pathway"(AOP)paradigm,a concept that links the individual events starting from the molecular initiating event(MIE) to the result at the level of the whole organism,integrated test strategy that take "weight of evidence" and "cost-effectiveness" into consideration for the AOP-based skin sensitization is the important goal for future.
Allergic contact dermatitis,or skin sensitization,is an inflammatory reaction controlled by the immune response induced by foreign substances.Animal models such as the guinea pig maximization test were traditionally used to estimate whether a chemical has the potential to induce skin sensitization in humans.Under the promote if 3Rs principle and modernization technologies,several refined animal testing and in vitro screening methods have been developed for identifying potential contact allergens,such as the murine local lymph node assay(LLNA),DPRA,KeratinoSens? and h-CLAT.The development of in vitro methods is suitable to predict sensitization hazard from chemicals and cosmetics.More recently,the theory underlying this approach has been formalized in the "adverse outcome pathway"(AOP)paradigm,a concept that links the individual events starting from the molecular initiating event(MIE) to the result at the level of the whole organism,integrated test strategy that take "weight of evidence" and "cost-effectiveness" into consideration for the AOP-based skin sensitization is the important goal for future.
引文
[1]程树军,焦红.实验动物替代方法原理与应用[M].北京:科学出版社,2010:285-304.
[2]OECD.The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins.Part 2:Use of the AOP to Develop Chemical Categories and Integrated Assessment and Testing Approaches.Series on Testing and Assessment No.168.Paris,2012.ENV/JM/MONO(2012)/PART2.
[3]Natsch A,Ryan CA,Foertsch L,et al.A dataset on145 chemicals tested in alternative assays for skin sensitization undergoing prevalidation[J].J Appl Toxicol,2013,33:1337-1352.
[4]Chaudhry Q,Piclin N,Cotterill J,et al.Global QSAR models of skin sensitisers for regulatory purposes[J].Chemistry Central Journal,2010,4(Suppl 1):S5.
[5]Coralie D,Pilar P,et al.Review of the Availability of In Vitro and In Silico Methods for Assessing Dermal Bioavailability[J].APPLIED IN VITRO TOXICOLOGY,2015,1(2):147-164.
[6]Ramirez T,Mehling A,Kolle SN,et al.LuSens:A keratinocyte based ARE reporter gene assay for use in integrated testing strategies for skin sensitization hazard identification[J].Toxicology in Vitro,2014,28:1482-1497.
[7]Sakaguchi H,Ashikaga T,Miyazawa M,et al.The relationship between CD86/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test-human cell line activation test(h-CLAT)[J].Cell Biol Toxicol,2009,25(2):109-126.
[8]Bauch C,Kolle SN,Ramirez T,et al.Putting the parts together:Combining in vitro methods to test for skin sensitizing potentials[J].Journal of Applied Toxicology,2012,63:489-504.
[9]Wong CL,Ghassabian S,Smith MT,et al.In vitro methods for hazard assessment of industrial chemicals-opportunities and challenges[J].Front.Pharmacol,2015,6:94.
[10]McKim JM Jr,Keller DJ III,Gorski JR.An in vitro method for detecting chemical sensitization using human reconstructed skin models and its applicability to cosmetic,pharmaceutical,and medical device safety testing[J].Cutan Ocul Toxicol,2012,31(4):292-305.
[11]Richter A,Schmucker SS,Esser PR,et al.Human T cell priming assay(hTCPA)for the identification of contact allergens based on naive T cells and DC-IFN-gamma and TNF-alpha readout[J].Toxicol.In Vitro,2013,27(3):1180-1185.
[12]Urbisch D,Mehling A,Katharina G,et al.Assessing skin sensitization hazard in mice and men using non-animal test methods[J].Regul Toxicol Pharmacol,2015,71(2):337-351.
[13]Jaworska J,Dancik Y,Kern P,et al.Bayesian integrated testing strategy to assess skin sensitization potency:from theory to practice[J].J Appl Toxicol,2013,33(11):1353-1364.
[14]Tsujita-Inoue KT,Hirota M,Ashikaga T,et al.Skin sensitization risk assessment model using artificial neural network analysis of data from multiple in vitro assays[J].Toxicol In Vitro,2014,28(4):626-639.
[15]Guilliams M,Henri S,Tamoutounour S,et al.From skin dendritic cells to a simplified classification of human and mouse dendritic cell subsets[J].Eur J Immunol,2010,40(8):2089-2094.
[16]Kinsner-Ovaskainen A,Maxwell G,Kreysa J,et al.Report of the EPAA-ECVAM workshop on the validation of integrated testing strategies(ITS)[J].Altern Lab Anim,2012,40:175-181.
[2]OECD.The Adverse Outcome Pathway for Skin Sensitisation Initiated by Covalent Binding to Proteins.Part 2:Use of the AOP to Develop Chemical Categories and Integrated Assessment and Testing Approaches.Series on Testing and Assessment No.168.Paris,2012.ENV/JM/MONO(2012)/PART2.
[3]Natsch A,Ryan CA,Foertsch L,et al.A dataset on145 chemicals tested in alternative assays for skin sensitization undergoing prevalidation[J].J Appl Toxicol,2013,33:1337-1352.
[4]Chaudhry Q,Piclin N,Cotterill J,et al.Global QSAR models of skin sensitisers for regulatory purposes[J].Chemistry Central Journal,2010,4(Suppl 1):S5.
[5]Coralie D,Pilar P,et al.Review of the Availability of In Vitro and In Silico Methods for Assessing Dermal Bioavailability[J].APPLIED IN VITRO TOXICOLOGY,2015,1(2):147-164.
[6]Ramirez T,Mehling A,Kolle SN,et al.LuSens:A keratinocyte based ARE reporter gene assay for use in integrated testing strategies for skin sensitization hazard identification[J].Toxicology in Vitro,2014,28:1482-1497.
[7]Sakaguchi H,Ashikaga T,Miyazawa M,et al.The relationship between CD86/CD54 expression and THP-1 cell viability in an in vitro skin sensitization test-human cell line activation test(h-CLAT)[J].Cell Biol Toxicol,2009,25(2):109-126.
[8]Bauch C,Kolle SN,Ramirez T,et al.Putting the parts together:Combining in vitro methods to test for skin sensitizing potentials[J].Journal of Applied Toxicology,2012,63:489-504.
[9]Wong CL,Ghassabian S,Smith MT,et al.In vitro methods for hazard assessment of industrial chemicals-opportunities and challenges[J].Front.Pharmacol,2015,6:94.
[10]McKim JM Jr,Keller DJ III,Gorski JR.An in vitro method for detecting chemical sensitization using human reconstructed skin models and its applicability to cosmetic,pharmaceutical,and medical device safety testing[J].Cutan Ocul Toxicol,2012,31(4):292-305.
[11]Richter A,Schmucker SS,Esser PR,et al.Human T cell priming assay(hTCPA)for the identification of contact allergens based on naive T cells and DC-IFN-gamma and TNF-alpha readout[J].Toxicol.In Vitro,2013,27(3):1180-1185.
[12]Urbisch D,Mehling A,Katharina G,et al.Assessing skin sensitization hazard in mice and men using non-animal test methods[J].Regul Toxicol Pharmacol,2015,71(2):337-351.
[13]Jaworska J,Dancik Y,Kern P,et al.Bayesian integrated testing strategy to assess skin sensitization potency:from theory to practice[J].J Appl Toxicol,2013,33(11):1353-1364.
[14]Tsujita-Inoue KT,Hirota M,Ashikaga T,et al.Skin sensitization risk assessment model using artificial neural network analysis of data from multiple in vitro assays[J].Toxicol In Vitro,2014,28(4):626-639.
[15]Guilliams M,Henri S,Tamoutounour S,et al.From skin dendritic cells to a simplified classification of human and mouse dendritic cell subsets[J].Eur J Immunol,2010,40(8):2089-2094.
[16]Kinsner-Ovaskainen A,Maxwell G,Kreysa J,et al.Report of the EPAA-ECVAM workshop on the validation of integrated testing strategies(ITS)[J].Altern Lab Anim,2012,40:175-181.