超高效液相色谱-荧光法检测乳腺癌大鼠尿液中对羟苯基乳酸、对羟苯基乙酸和对羟苯基丙酸
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摘要
<正>酪氨酸是人体重要的半必需氨基酸,人体液中氨基酸及其代谢产物的含量(对羟苯基乳酸、对羟苯基乙酸和对羟苯基丙酸等)与多种疾病相关,而且研究表明其与乳腺癌等恶性肿瘤有一定的相关性[1-3]。进一步研究体液中酪氨酸及其代谢产物与恶性肿瘤的相干性,寻找恶性肿瘤特异性生物标志物,对于恶性肿瘤的早期筛查与诊断具有非常重要的意义。本文采用商品化固相萃取柱去除尿样中杂质,使用离子对试剂增强目标物在反相色谱柱
引文
1.Lei Feng,Yuan-Qiao He,Guo-Hui Xu,et al.Determination of Tyrosine and Its Metabolites in Human Serum with Application to Cancer Diagnosis[J].Analytical Letters,2014,47(8):1275-1289
2 .Felitsyn N M,Henderson G N,James M O,et al.Liquid chromatography–tandem mass spectrometry method for the simultaneous determination ofδ-ALA,tyrosine and creatinine in biological fluids[J].Clinica Chimica Acta,2004,350(s 1–2):219-230
3 .Mu S,Ying L,Tang A G,et al.Simultaneous determination of tyrosine,tryptophan and 5-hydroxytryptamine in serum of MDD patients by high performance liquid chromatography with fluorescence detection[J].Clinica Chimica Acta,2012,413(s 11–12):973-977
2 .Felitsyn N M,Henderson G N,James M O,et al.Liquid chromatography–tandem mass spectrometry method for the simultaneous determination ofδ-ALA,tyrosine and creatinine in biological fluids[J].Clinica Chimica Acta,2004,350(s 1–2):219-230
3 .Mu S,Ying L,Tang A G,et al.Simultaneous determination of tyrosine,tryptophan and 5-hydroxytryptamine in serum of MDD patients by high performance liquid chromatography with fluorescence detection[J].Clinica Chimica Acta,2012,413(s 11–12):973-977